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Protein & Peptide Letters


ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Research Article

Oral Angiotensin-(1-7) Peptide Modulates Intestinal Microbiota Improving Metabolic Profile in Obese Mice

Author(s): Amanda Machado, Janaína Ribeiro Oliveira, Deborah de F. Lelis, Victor Hugo D. Guimarães, Alfredo M.B. de Paula, Andre L.S. Guimarães, Igor V. Brandi, Bruna Mara A. de Carvalho, Diego Vicente da Costa, Cláudia Regina Vieira, Ulisses Alves Pereira, Theles de Oliveira Costa, João M.O. Andrade, Robson Augusto Souza Santos and Sérgio H.S. Santos*

Volume 28 , Issue 10 , 2021

Published on: 16 August, 2021

Page: [1127 - 1137] Pages: 11

DOI: 10.2174/0929866528666210816115645

Price: $65


Background: Obesity is a serious health problem that dysregulate Renin-Angiotensin System (RAS) and intestinal microbiota.

Objective: The present study aimed to evaluate the Angiotensin-(1-7) [ANG-(1-7)] oral formulation effects on obese mice intestinal microbiota.

Methods: Mice were divided into four groups: obese and non-obese treated with ANG-(1-7) and obese and non-obese without ANG-(1-7) during four weeks.

Results: We observed a significant decrease in the fasting plasma glucose, total cholesterol, triglycerides, and Low-density lipoprotein levels and increased High-density lipoprotein in animals treated with ANG-(1-7). The histological analysis showed intestinal villi height reduction in mice treated with ANG-(1-7). Additionally, increased Bacteroidetes and decreased Firmicutes (increased Bacteroidetes/ Firmicutes ratio) and Enterobacter cloacae populations were observed in the High-Fat Diet + ANG-(1-7) group. Receptor toll-like 4 (TLR4) intestinal mRNA expression was reduced in the HFD+ANG-(1-7) group. Finally, the intestinal expression of the neutral amino acid transporter (B0AT1) was increased in animals treated with ANG-(1-7), indicating a possible mechanism associated with tryptophan uptake.

Conclusion: The results of the present study suggest for the first time an interaction between oral ANG-(1-7) and intestinal microbiota modulation.

Keywords: Gut microbiota, metabolism, renin-angiotensin system, metabolic endotoxemia, small intestine, ACE2.

Graphical Abstract
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