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ISSN (Print): 2666-1454
ISSN (Online): 2666-1462

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Research Article

Antibacterial Activity of Indian Spices against the Pathogens Isolated from Wound Infection

Author(s): Saruchi, Anjali Saini and Vaneet Kumar*

Volume 15, Issue 1, 2022

Published on: 08 June, 2021

Page: [38 - 51] Pages: 14

DOI: 10.2174/2666145414666210608123711

Price: $65

Abstract

Introduction: Plants have been used for thousands of years to treat health disorders, to prevent diseases including epidemics, to flavor and conserve food. It is estimated that 250 to 500 thousand plant species are present on the earth, out of which merely 10 percent are used as a source of food by humans and animals.

Objective: In the present work, antibacterial activity of five different Indian spices namely turmeric, clove, pepper, cinnamon and garlic was investigated against pathogens isolated from wound samples

Methods: The unknown bacteria were identified by different types of morphological and biochemical techniques such as serial dilutions spread plate methods, morphological studies and biochemical tests. The swabs of the patients were inoculated in 10 mL of sterile nutrient broth and incubated at 37oC for 24 h. The antibacterial activities of these Indian spices were evaluated using the disk diffusion method. A suspension of the microorganisms to be tested was spread on nutrient agar and MacConkey agar medium. The filter paper discs were placed on the agar plates, which were saturated with extract of spice. The plates were then incubated at 37oC for 24 h. The zone of inhibition was determined post-incubation by evaluating the diameter of the zone of inhibition.

Results: The antibacterial activities of these Indian spices were evaluated using the disk diffusion method, and the inhibitory zones were recorded. It was quite evident from the result that cinnamon had a larger inhibition zone against P1NA3, P1MAC1 and least for P2NA2. Garlic exhibited a greater zone of inhibition against P2NA2, P3NA3 and least for P1MAC1. Clove had a maximum zone of inhibition against P1MAC2 and least with P3NA3. These spices displayed a zone of inhibition somewhat close to the control antibiotic drug, ampicillin.

Conclusion: P1MNC2 showed a maximum zone of inhibition with clove (3 cm) at 100%. P2NA2 showed maximum antibacterial activity with garlic (3.6 cm) at 100% and minimum with cinnamon (1.1cm) at 20%. P3NA3 presented maximum antibacterial activity result with garlic (4.2 cm) at 100% and minimum with clove (1.2cm) at 20%. Spices are economical, more accessible to most of the population in the world. So, medicinal plants should be encouraged as potential candidates for new drugs.

Keywords: Antibacterial activity, Indian spices, pathogens, isolation, disk diffusion method, inhibition zone.

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[1]
Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999; 12(4): 564-82.
[http://dx.doi.org/10.1128/CMR.12.4.564] [PMID: 10515903]
[2]
Makhija I, Khamar D. Anti-snake venom properties of medicinal plants. Der Pharmacia Lettre 2010; 2: 399-411.
[3]
Calixto JB. Twenty-five years of research on medicinal plants in Latin America: a personal view. J Ethnopharmacol 2005; 100(1-2): 131-4.
[http://dx.doi.org/10.1016/j.jep.2005.06.004] [PMID: 16006081]
[4]
Chowdhary K, Kaushik N, Gonzalez-Coloma A, Raimundo CM. Endophytic fungi and their metabolites isolated from Indian medicinal plant. Phytochem Rev 2012; 11(4): 467-85.
[http://dx.doi.org/10.1007/s11101-012-9264-2]
[5]
Adhikari R, Kumar HN, Shruthi SD. A review on medicinal importance of Basella alba L. Int J Pharm Sci Drug Res 2012; 4(2): 110-4.
[6]
Ahmad N, Alam MK, Shehbaz A, et al. Antimicrobial activity of clove oil and its potential in the treatment of vaginal candidiasis. J Drug Target 2005; 13(10): 555-61.
[http://dx.doi.org/10.1080/10611860500422958] [PMID: 16390816]
[7]
Ahmad N, Fazal H, Abbasi BH, Farooq S, Ali M, Khan MA. Biological role of Piper nigrum L. (Black pepper): a review. Asian Paci J Trop Biomed 2012; S1945-53.
[http://dx.doi.org/10.1016/S2221-1691(12)60524-3]
[8]
Beuchat LR. Antimicrobial properties of spices and their essential oils Natural Antimicrobial systems and food preservation. Oxon: CAB International 1999; pp. 167-79.
[9]
Booyens J. Antibacterial activity of garlic (allium sativum) against probiotic bifidobacterium species.Dissertation (MSc). University of Pretoria 2013.
[10]
Brul S, Coote P. Preservative agents in foods. Mode of action and microbial resistance mechanisms. Int J Food Microbiol 1999; 50(1-2): 1-17.
[http://dx.doi.org/10.1016/S0168-1605(99)00072-0] [PMID: 10488839]
[11]
Cavallito CJ, Baily JH. Allicin, the antibacterial principle of Allium sativum isolation, physical properties and antibacterial action. J Am Chem Soc 1994; 66: 1950.
[http://dx.doi.org/10.1021/ja01239a048]
[12]
Cortés-Rojas DF, de Souza CR, Oliveira WP. Clove (Syzygium aromaticum): a precious spice. Asian Pac J Trop Biomed 2014; 4(2): 90-6.
[http://dx.doi.org/10.1016/S2221-1691(14)60215-X] [PMID: 25182278]
[13]
Daka D. Antibacterial effect of garlic (Allium sativum) on Staphyloccus aureus: an in vitro study. Afr J Biotechnol 2011; 10(4): 666-9.
[14]
Budhathoki S, Basnet A. Study of Antibacterial activity of household spices. Int J Sci Eng Res 2018; 9(8): 1700-4.
[15]
Dhiman R, Aggarwal N, Aneja KR, Kaur M. In vitro antimicrobial activity of spices and medicinal herbs against selected microbes associated with juices. Int J Microbio 2016; 1-10.
[16]
Damanhouri ZA, Ahmad A. Therapeutic potential of Piper nigrum L. (black pepper): the king of spices. damanhouri and ahmad. Med Aromat Plants 2014; 3: 3-10.
[http://dx.doi.org/10.4172/2167-0412.1000161]
[17]
Dash BK, Sultana S, Sultana N. Antibacterial activities of methanol and acetone extracts of fenugreek (trigonella foenum) and coriander (coriandrum sativum). Lif Sci Med Res 2011.
[18]
Elsom GK, Hide D, Salmon DM. An antibacterial assay of aqueous extract of garlic against anaerobic/microaerophilic and aerobic bacteria. Mic Eco Heal Dise 2000; 12: 81-4.
[19]
Eltaweel A. Antibacterial effect of garlic (allium sativum) on Staphyloccus aureus. An in vitro study. Int’l conf on advances in environment, agriculture & medical sciences (ICAEAM’14). November 16-17 2014.
[20]
Ganesh P, Kumar RS, Saranraj P. Phytochemical analysis and antibacterial activity of Pepper (Piper nigrum L.) against some human pathogens. Cent Europ J Exp Bio 2014; 3: 36-41.
[21]
Saruchi Thakur P, Kumar V. Kinetics and thermodynamic studies for removal of methylene blue dye by biosynthesize copper oxide nanoparticles and its antibacterial activity. J Environ Health Sci Eng 2019; 17(1): 367-76.
[http://dx.doi.org/10.1007/s40201-019-00354-1] [PMID: 31322630]
[22]
Gebreyohannes G, Gebreyohannes M. Medicinal values of garlic. Int J Med Medical Sci 2013; 5(9): 401-8.
[23]
Gutierrez J, Barry-Ryan C, Bourke P. The antimicrobial efficacy of plant essential oil combinations and interactions with food ingredients. Int J Food Microbiol 2008; 124(1): 91-7.
[http://dx.doi.org/10.1016/j.ijfoodmicro.2008.02.028] [PMID: 18378032]
[24]
Hoquea M, Barib ML, Junejac VK, Kawamotob S. Antimicrobial activity of cloves and cinnamon extracts against food borne pathogens and spoilage bacteria, and inactivation of Listeria monocytogenes in ground chicken meat with their essential oils. Rep Nat’l Food Res Inst 2008; 72: 9-21.
[25]
Hugar S, Metgud R. Turmeric in Dentistry. Int J Sci Res 2013.
[26]
Jakhetia V. Cinnamon: a pharmacological review. J Adv Sci Res 2010; 1(2): 19-23.
[27]
Karsha PV, Lakshmi OB. Antibacterial activity of black pepper (Piper nigrum) with special reference to its mode of action. Ind J Nat Prod Res 2010; 1(2): 213-5.
[28]
Hara-Kudo Y, Kobayashi A, Sugita-Konishi Y, Kondo K. Antibacterial activity of plants used in cooking for aroma and taste. J Food Prot 2004; 67(12): 2820-4.
[http://dx.doi.org/10.4315/0362-028X-67.12.2820] [PMID: 15633695]
[29]
Kumar Y, Agarwal S, Srivastava A, Kumar S, Agarwal G, Khan MZA. Antibacterial activity of Clove (Syzygium aromaticum) and Garlic (Allium sativum) on different pathogenic bacteria. Int J Pure App Biosci 2014; 2(3): 305-11.
[30]
Lal J. Turmeric, curcumin and our life bulletin of environment. Pharm Life Sci 2012; 1(7): 11-7.
[31]
López P, Sánchez C, Batlle R, Nerín C. Solid- and vapor-phase antimicrobial activities of six essential oils: susceptibility of selected foodborne bacterial and fungal strains. J Agric Food Chem 2005; 53(17): 6939-46.
[http://dx.doi.org/10.1021/jf050709v] [PMID: 16104824]
[32]
Mohanty JP, Nath LK, Bhuyan NR, Mahapatra SK. Antibacterial spectrum of Kaempferia rotunda Linn. and Eupatorium cannabium Linn. Adv Pharmacol Toxicol 2008; 9: 45-50.
[33]
Nabavi SF, Di Lorenzo A, Izadi M, Sobarzo-Sánchez E, Daglia M, Nabavi SM. Antibacterial effects of cinnamon: from farm to food, cosmetic and pharmaceutical industries. Nutrients 2015; 7(9): 7729-48.
[http://dx.doi.org/10.3390/nu7095359] [PMID: 26378575]
[34]
Nuñez L, Aquino MD. Microbicide activity of clove essential oil (Eugenia caryophyllata). Braz J Microbiol 2012; 43(4): 1255-60.
[http://dx.doi.org/10.1590/S1517-83822012000400003] [PMID: 24031950]
[35]
Oussalah M, Caillet S, Saucier L, Lacroix M. Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria: E. coli O157:H7, Salmonella Typhimurium, Staphyloccus aureus and teria monocytogenes. Food Control 2006; 5: 414-20.
[36]
Smith-Palmer A, Stewart J, Fyfe L. Antimicrobial properties of plant essential oils and essences against five important food-borne pathogens. Lett Appl Microbiol 1998; 26(2): 118-22.
[http://dx.doi.org/10.1046/j.1472-765X.1998.00303.x] [PMID: 9569693]
[37]
Panday A, Singh P. Antibacterial activity of Syzygium aromaticum (clove) with metal ion effect against food borne pathogens. Asi J Pla Sci Res 2011; 1(2): 69-80.
[38]
Parekhm PJ, Chandam SV. In vitro antimicrobial avtivity and phytochemical analysis of some indian medicinal plants. Turk J Biol 2007; 31: 53-8.
[39]
Park JE, Choi HJ, Jung SH, Kim NJ, Kim DH. East-West medicinal plants of Korea. J Pharmacogn 2004; 32: 257-68.
[40]
Prasad S, Aggarwal BB. Turmeric the golden spice, from traditional medicine to modern medicine. In: Benzie IFF, Wachtel-Galor S, Eds. Herbal Medicine: Biomolecular and Clinical Aspects. 2nd ed. Boca Raton, (FL): CRC Press/Taylor & Francis 2011.
[41]
Premanath R, Sudaisha J, Devi NL, Aradhya SM. Anatibacterial and antioxidant activities of fenugreek leaves. Res J Med Plant 2011; 5(6): 695-705.
[http://dx.doi.org/10.3923/rjmp.2011.695.705]
[42]
Rani SKS, Saxena N. Udaysree. Antimicrobial activity of black pepper (Piper nigrum L.). Glo J Pharm 2013; 7(1): 87-90.
[43]
Ruengvisesh S. The effect of curcumin (Curcuma longa) on biofilm formation and surface proteins of listeria monocytogenes. Food Sci Comm 2012; 6(4): 274-9.
[44]
Silva NCC, Fernandes JA. Biological properties of medicinal plants: a review of their antimicrobial activity. J Ven Ani Tox Trop Dis 2010; 16(3): 402-13.
[45]
Smid EJ, Gorris LGM. Natural antimicrobials for food preservation Handbook of Food Preservation. New York: Marcel Dekker 1999; pp. 285-308.
[46]
Srinath J, Lakshmi T. Application of spices in dentistry- a literature review. Int J Drug Dev Res 2014; 6(2): 1-9.
[47]
Naushad M. Surfactant assisted nano-composite cation exchanger: Development, characterization and applications for the removal of toxic Pb2+ from aqueous medium. Chem Eng J 2014; 235: 100-8.
[http://dx.doi.org/10.1016/j.cej.2013.09.013]
[48]
Naushad M, Mittal A, Rathore M, Gupta V. Ion-exchange kinetic studies for Cd(II), Co(II), Cu(II), and Pb(II) metal ions over a composite cation exchanger, Desalin. Water Treat 2015; 54: 2883-90.
[http://dx.doi.org/10.1080/19443994.2014.904823]

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