Generic placeholder image

Current Bioinformatics


ISSN (Print): 1574-8936
ISSN (Online): 2212-392X

Research Article

Predicting Interactions Between Pathogen and Human Proteins Based on the Relation Between Sequence Length and Amino Acid Composition

Author(s): Saud Alguwaizani, Shulei Ren, De-Shuang Huang and Kyungsook Han*

Volume 16 , Issue 6 , 2021

Published on: 30 April, 2021

Page: [799 - 806] Pages: 8

DOI: 10.2174/1574893616666210430133846

Price: $65


Aim: Both bacterial infection and viral infection involve a large number of protein-protein interactions (PPIs) between a pathogen and its target host.

Background: So far, many computational methods have focused on predicting PPIs within the same species rather than PPIs across different species.

Methods: From the extensive analysis of PPIs between Yersinia pestis bacteria and humans, we recently discovered an interesting relation; a linear relation between amino acid composition and sequence length was observed in many proteins involved in PPIs. We have built a support vector machine (SVM) model, which predicts PPIs between human and bacteria using two feature types derived from the relation. The two feature types used in the SVM are the amino acid composition group (AACG) and the difference in amino acid composition between host and pathogen proteins.

Results: The SVM model achieved high performance in predicting bacteria-human PPIs. The model showed an accuracy of 96%, sensitivity of 94%, and specificity of 98% in predicting PPIs between humans and Yersinia pestis, in which there is a strong relation between amino acid composition and sequence length. The SVM model was also tested in predicting PPIs between human and viruses, which include Ebola, HCV, and SARS-CoV-2, and showed a good performance.

Conclusion: The feature types identified in our study are simple yet powerful in predicting pathogenhuman PPIs. Although preliminary, our method will be useful for finding unknown target host proteins or pathogen proteins and designing in vitro or in vivo experiments.

Keywords: Protein-protein interaction, pathogen-host interaction, machine learning, Y. pestis, Ebola, HCV, SARS-CoV-2.

Graphical Abstract

Rights & Permissions Print Export Cite as
© 2022 Bentham Science Publishers | Privacy Policy