Immobilization of Amano AK Lipase from Pseudomonas fluorescens on Novel Silk Microfiber using Oxone®: Parameter Optimization for Enzymatic Assays and use in Esterification of Residual Palm Oil

Title:Immobilization of Amano AK Lipase from Pseudomonas fluorescens on Novel Silk Microfiber using Oxone®: Parameter Optimization for Enzymatic Assays and use in Esterification of Residual Palm Oil

Volume: 10 Issue: 2

Author(s): Evaldo B.M. Júnior*, Fernando B. Neves, Samuel Q. Lopes , Fabrício H. Holanda , Tiago M. Souza , Erveton P. Pinto, Alex N. Oliveira, Luis P. Fonseca, Sérgio A. Yoshioka and Irlon M. Ferreira

Affiliation:

• Laboratório de Biocatalise e Sintese Organica Aplicada, Universidade Federal do Amapa, Rod. Juscelino Kubitschek, Km 02, Jardim Marco Zero, 68902-280, Macapa, AP,Brazil

Keywords: Pseudomonas fluorescens, microfibrils, silk fibroin, oxone®, deodorization distillate palm oil, amano ak lipase.

Abstract:

Background: Biodiesel has been shown to be effectively produced by immobilized enzymatic catalysts. The selection of support material is a prominent factor for obtaining an efficient lipase. Silk fibroin (SF) is a natural polymer produced by glands of some arthropods, especially by the Bombyx mor, attracting attention for immobilization of lipase.

Objective: This paper presents a novel method to obtain silk microfibers (SMF) from Oxone® salt in water, used as support for Amano AK lipase from Pseudomonas fluorescens in biodiesel production from deodorization distillate of palm oil (DDPO).

Method: The oxone® salt in the presence of Ca2+ ions acts as a mineralizing agent in the peptide bonds present in silk fibroin, altering some of its physical and chemical properties, such as zeta potential, crystallinity, micro-morphology, infrared spectroscopic profile, and showing formation or absence of SF original connections.

Results: The modified support was tested as a support alternative for the immobilization of Amano AK lipase from Pseudomonas fluorescens. Enzyme activity values indicated that lipase immobilization on SMF was efficient as a heterogeneous catalyst in the esterification of DDPO (deodorization distillate palm oil).

Conclusion: The effect of some reaction parameters, such as catalyst concentration, molar ratio, temperature, and reaction time, was studied to optimize the conditions for maximum conversion of DDPO (40.5%).

Cite this article as:

Júnior B.M. Evaldo *, Neves B. Fernando , Lopes Q. Samuel , Holanda H. Fabrício , Souza M. Tiago, Pinto P. Erveton , Oliveira N. Alex , Fonseca P. Luis, Yoshioka A. Sérgio and Ferreira M. Irlon , Immobilization of Amano AK Lipase from Pseudomonas fluorescens on Novel Silk Microfiber using Oxone®: Parameter Optimization for Enzymatic Assays and use in Esterification of Residual Palm Oil, Current Catalysis 2021; 10 (2) . https://dx.doi.org/10.2174/2211544710666210401111704