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Current Catalysis

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ISSN (Print): 2211-5447
ISSN (Online): 2211-5455

Research Article

Immobilization of Amano AK Lipase from Pseudomonas fluorescens on Novel Silk Microfiber using Oxone®: Parameter Optimization for Enzymatic Assays and use in Esterification of Residual Palm Oil

Author(s): Evaldo B.M. Júnior*, Fernando B. Neves, Samuel Q. Lopes , Fabrício H. Holanda , Tiago M. Souza , Erveton P. Pinto, Alex N. Oliveira, Luis P. Fonseca, Sérgio A. Yoshioka and Irlon M. Ferreira

Volume 10 , Issue 2 , 2021

Published on: 01 April, 2021

Page: [119 - 129] Pages: 11

DOI: 10.2174/2211544710666210401111704

Price: $65

Abstract

Background: Biodiesel has been shown to be effectively produced by immobilized enzymatic catalysts. The selection of support material is a prominent factor for obtaining an efficient lipase. Silk fibroin (SF) is a natural polymer produced by glands of some arthropods, especially by the Bombyx mor, attracting attention for immobilization of lipase.

Objective: This paper presents a novel method to obtain silk microfibers (SMF) from Oxone® salt in water, used as support for Amano AK lipase from Pseudomonas fluorescens in biodiesel production from deodorization distillate of palm oil (DDPO).

Method: The oxone® salt in the presence of Ca2+ ions acts as a mineralizing agent in the peptide bonds present in silk fibroin, altering some of its physical and chemical properties, such as zeta potential, crystallinity, micro-morphology, infrared spectroscopic profile, and showing formation or absence of SF original connections.

Results: The modified support was tested as a support alternative for the immobilization of Amano AK lipase from Pseudomonas fluorescens. Enzyme activity values indicated that lipase immobilization on SMF was efficient as a heterogeneous catalyst in the esterification of DDPO (deodorization distillate palm oil).

Conclusion: The effect of some reaction parameters, such as catalyst concentration, molar ratio, temperature, and reaction time, was studied to optimize the conditions for maximum conversion of DDPO (40.5%).

Keywords: Pseudomonas fluorescens, microfibrils, silk fibroin, oxone®, deodorization distillate palm oil, amano ak lipase.

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