Background: The objective of this research was to screen metabolites with specificity differences in the lung tissue of paraquat-poisoned rats by metabolomics technology and chi-square test method, to provide a theoretical basis for the study of the mechanisms of paraquat poisoning, and to use machine learning technology to construct a paraquat poisoning diagnosis model. This provided an intelligent decision-making method for the diagnosis of paraquat poisoning.
Methods: 18 paraquat-poisoned rats (36 mg/kg) and 16 positive control rats were selected. Lung tissue from each rat from both groups was extracted and analyzed by GC-MS. The chi-square test for feature evaluation was used to screen the difference in specific metabolites in the lung tissue between the paraquat-poisoned rats and the control group, and the SVM classification machine learning algorithm was used to construct an intelligent diagnosis model.
Results: In the end, a total of 14 significant metabolic differences were identified between the two groups (P < 0.05). The sensitivity, specificity, and accuracy of the constructed SVM paraquat poisoning diagnostic model reached 95%, 95% and 96.67%, respectively.
Conclusion: Based on metabolomics technology, the chi-square test for feature evaluation was used to successfully screen the changes of specific metabolites produced in the lungs after paraquat- poisoning, and the diagnosis model based on SVM was constructed to provide an intelligent decision for the diagnosis of paraquat poisoning.
[http://dx.doi.org/10.1007/s11306-019-1611-5] [PMID: 31696341]
[http://dx.doi.org/10.1088/1752-7163/abaeca] [PMID: 32969349]
[http://dx.doi.org/10.1039/D0AY00968G] [PMID: 33001064]
[http://dx.doi.org/10.1021/acs.chemrestox.8b00328] [PMID: 30807114]