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Current Bioactive Compounds


ISSN (Print): 1573-4072
ISSN (Online): 1875-6646

Research Article

Effect of Solvent on Antioxidant Activity of Zanthoxylum oxyphyllum Edgew and its DFT Study

Author(s): Nilamoni Chelleng, Moushumi Hazarika, Amlan J. Kalita, Ankur K. Guha and Chandan Tamuly*

Volume 17, Issue 10, 2021

Published on: 22 February, 2021

Article ID: e190721191725 Pages: 8

DOI: 10.2174/1573407217666210223105051

Price: $65


Background: Free radicals can easily damage DNA, proteins and lipids within the tissue. Anti-oxidants from natural sources can diminish the actions of free radicals. The current study deals with the chemical composition and antioxidant activity of the leaves of Zanthoxylum oxyphyllum Edgew.

Methods: The antioxidant activities for DPPH, FRAP, ABTS radicals, phosphomolybdate assay, reducing power, and chelating power assay were evaluated of ethanol (ET), methanol (ME), chloroform (CH), ethyl acetate (EA), and petroleum ether (PE) extracts of the leaves. The Density Functional Theory (DFT) study was carried out on major identified phytochemicals to evaluate bioactive molecules responsible for the antioxidant activity.

Results: It was observed that the ME extract showed the most potent scavenging activity in DPPH, FRAP, ABTS radicals, phosphomolybdate assay, reducing power, and chelating power assay. The phenolic acids and flavonoids like quercetin, gallic acid, sinapic acid, etc. were identified. The DFT study was done for major phytochemicals of ME extract to evaluate the most responsible bioactive molecule for antioxidant activity. The Gallic acid exhibited the lowest bond dissociation energy (BDE) of 314.9 kcal/mol in gas, 309.2 kcal/mol in methanol, respectively, along with the highest value of radical stabilization energy (RSE), 29.5 kcal/mol.

Conclusion: Gallic acid was found to be the most responsible antioxidant among the other compounds, and ME the best solvent system for extraction, followed by CH.

Keywords: Antioxidant capacity, DPPH, FRAP, methanol, bioactive molecules, Zanthoxylum oxyphyllum.

Graphical Abstract
Baskar, A.A.; Manoharan, S.; Manivasagam, T.; Subramanian, P. Temporal patterns of lipid peroxidation product formation and antioxidants activity in oral cancer patients. Cell. Mol. Biol. Lett., 2004, 9(4A), 665-673.
[PMID: 15647789]
Vasundhara, S.; Vijay Kumar, H.; Jagan Mohan Rao, J. Influence of milk and sugar on antioxidant potential of black tea. Food Res. Int., 2008, 41, 124-129.
Sarma, H.K.; Gogoi, B.; Nainwal, L.M. A review on some antioxidant plant species growing in North East India Int. J. Pharma. Sci. Rev. & Res., 2016, 37(1), 224-229.
Tamuly, C.; Dutta, P.P.; Bordoloi, M.; Bora, J. Antifungal and antioxidant pyrrole derivative from Piper pedicellatum. Nat. Prod. Commun., 2013, 8(10), 1451-1454.
[] [PMID: 24354199]
Tamuly, C.; Saikia, B.; Hazarika, M.; Bora, J.; Bordoloi, M.J.; Sahu, O.P. Correlation between phenolic, flavonoid, and mineral contents with antioxidant activity of underutilized vegetables. Int. J. Veg. Sci., 2013, 19(1), 34-44.
Tamuly, C.; Hazarika, M.; Bora, J.; Gajurel, P.R. Antioxidant activities and phenolic content of Piper wallichii (Miq.) Hand.- Mazz. Int. J. Food Prop., 2014, 17(2), 309-320.
Tamuly, C.; Hazarika, M.; Bora, J.; Bordoloi, M.; Boruah, M.P.; Gajurel, P.R. In vitro study on antioxidant activity and phenolic content of three Piper species from North East India. J. Food Sci. Technol., 2015, 52(1), 117-128.
Patiri, B.; Borah, A. Wild edible plant species in patch vegetations of Jorhat district, Assam, India.Int. J. Biologic. Sci; , 2017, 6, pp. (3)14-25.
Park, Y.S.; Jung, S.T.; Kang, S.G.; Heo, B.K.; Arancibia-Avila, P.; Toledo, F.; Drzewiecki, J.; Namiesnik, J.; Gorinstein, S. Antioxidants and proteins in hosphor-treated kiwifruits. Food Chem., 2008, 107(2), 640-648.
Aoshima, H.; Tsunoue, H.; Koda, H.; Kiso, Y. Aging of whiskey increases 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. J. Agric. Food Chem., 2004, 52(16), 5240-5244.
[] [PMID: 15291502]
Re, R.; Pellegrini, N.; Proteggente, A.; Pannala, A.; Yang, M.; Rice-Evans, C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med., 1999, 26(9-10), 1231-1237.
[] [PMID: 10381194]
Benzie, I.F.; Strain, J.J.; Strain, J.J. Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol., 1999, 299, 15-27.
[] [PMID: 9916193]
Umamaheswari, M.; Chatterjee, T.K. In vitro antioxidant activities of the fractions of Coccinia grandis L. leaf extract. Afr. J. Tradit. Complement. Altern. Med., 2007, 5(1), 61-73.
[PMID: 20162057]
Oyaizu, M. Antioxidant activity of browning products of glucosamine fractionated by organic solvent and thin-layer chromatography. Nippon Shokuhin Kogyo Gakkaishi, 1986, 35, 771-775.
Dastmalchi, K.D.; Doeman, H.J.D.; Oinonen, P.P.; Darwis, Y.; Laaso, I.; Hiltunen, R. Chemical composition and in vitro antioxidative activity of a lemon balm (Melissa officinalis L.) extract. Lebensm. Wiss. Technol., 2008, 41, 391-400.
Zhao, Y.; Truhlar, D.G. Theor. Chem. Acc., 2008, 120, 215.
Glendening, E. D.; Reed, A. E.; Carpenter, J. E.; Weinhold, F. (2003) NBO Version 3.1; Gaussian Inc.: Pittsburgh, 1988. Available from: referencespapers.aspx?referenceid=1595007
Reed, A.E.; Weinhold, F.; Curtiss, L.A. Chem. Rev., 1988, 88, 899-926.
Frisch, M.J.; Trucks, G.W.; Schlegel, H.B.; Scuseria, G.E.; Robb, M.A.; Cheeseman, G.; Barone, V.; Petersson, G.A.; Nakatsuji, H. Gaussian 16, Revision A.03; , 2016. Available from:
Ao, C.; Li, A.; Elzaawely, A.A.; Xuan, D.T.; Twata, S. Evaluation of antioxidant and antibacterial activities of Ficus microcarpa L. fill extract. Food Control, 2008, 19, 940-948.
Erlund, I. Review of the flavonoids quercetin, hesperetin and naringenin. Dietary sources, bioactivities, bioavailability and epidemiology. Nutr. Res., 2004, 24, 851-874.

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