Generic placeholder image

Current Bioactive Compounds


ISSN (Print): 1573-4072
ISSN (Online): 1875-6646

Research Article

Chemical Composition Analysis and In Vivo Anti-diabetic Activity of Aqueous Extract of Aerial Part of Pallenis spinosa in Diabetic Rats

Author(s): Abdelkrim Khettaf* and Seloua Dridi

Volume 18, Issue 1, 2022

Published on: 22 February, 2021

Article ID: e010921191723 Pages: 7

DOI: 10.2174/1573407217666210223102639

Price: $65


Background: This work aims to confirm the anti-diabetic activity of Pallenis spinosa, as recommended in traditional medicine, and perform a chemical analysis of the aerial part of Pallenis spinosa.

Methods: The isolated compounds have been identified by chemical methods and spectrometric analysis, such as UV, 1H NMR, and 13C NMR spectroscopy. With regard to the anti-diabetic activity, a series of experiments have been carried out in vivo on Westar albino rats. Diabetes has been induced in animals by an intraperitoneal injection of streptozotocine. They have been treated with an aqueous extract from the aerial part of Pallenis spinosa (250 and 500 mg/kg body weight) and glibenclamide (5 mg/kg body weight) every day for 21 days.

Results: The chemical analysis of the aerial part of Pallenis spinosa led to the isolation of five known flavonoids, including patuletin 7-galactopyranoside, patuletin-3-O-α-L-rharnnopyranosyl (1-6)-β-D-galactopyranoside, tricin 7-glucopyranoside, tricin, and quercetin. The aqueous extract of both doses, 250 mg and 500 mg, has shown significant activity in reducing blood sugar, with 43.38% for the dose of 250 mg/kg and 37.76% for the dose of 500 mg/kg, as well as a significant decrease in the total fatness, triglycerides, and the total cholesterol levels in animals. The treatment was compared to the diabetes control group (p = 0.05). We used glibenclamide as a reference, and it showed similar results.

Conclusion: In order to explore and develop new anti-diabetic drugs, more studies are needed on this plant for scrutinizing its mechanism of activity.

Keywords: Pallenis spinosa, anti-diabetic, flavonoid, streptozotocin-induced diabetic, aqueous extract, triditional medicine.

Graphical Abstract
Baker, D.D.; Chu, M.; Oza, U.; Rajgarhia, V. The value of natural products to future pharmaceutical discovery. Nat. Prod. Rep., 2007, 24(6), 1225-1244.
[] [PMID: 18033577]
de Melo Júnior, E.J.; Raposo, M.J.; Lisboa Neto, J.A.; Diniz, M.F.; Marcelino Júnior, C.A.; Sant’Ana, A.E. Medicinal plants in the healing of dry socket in rats: Microbiological and microscopic analysis. Phytomedicine, 2002, 9(2), 109-116.
[] [PMID: 11995943]
Piedrola, G.; Novo, E.; Escobar, F.; Garcia-Robles, R. White blood cell count and insulin resistance in patients with coronary artery disease.Ann Endocrinol (Paris); , 2001, 62, pp. (1 Pt 1)7-10.
Yaryura-Tobias, J.A.; Pinto, A.; Neziroglu, F. Anorexia nervosa, diabetes mellitus, brain atrophy, and fatty liver. Int. J. Eat. Disord., 2001, 30(3), 350-353.
[] [PMID: 11746296]
Coniff, R.F.; Shapiro, J.A.; Seaton, T.B.; Bray, G.A. Multicenter, placebo-controlled trial comparing acarbose (BAY g 5421) with placebo, tolbutamide, and tolbutamide-plus-acarbose in non-insulin-dependent diabetes mellitus. Am. J. Med., 1995, 98(5), 443-451.
[] [PMID: 7733122]
Sharma, S.B.; Nasir, A.; Prabhu, K.M.; Murthy, P.S. Antihyperglycemic effect of the fruit-pulp of Eugenia jambolana in experimental diabetes mellitus. J. Ethnopharmacol., 2006, 104(3), 367-373.
[] [PMID: 16386863]
Kang, K.A.; Chae, S.; Koh, Y.S.; Kim, J.S.; Lee, J-H.; You, H.J.; Hyun, J.W. Protective effect of Puerariae radix on oxidative stress induced by hydrogen peroxide and streptozotocin. Biol. Pharm. Bull., 2005, 28(7), 1154-1160.
[] [PMID: 15997089]
Marles, R.J.; Farnsworth, N.R. Antidiabetic plants and their active constituents. Phytomedicine, 1995, 2(2), 137-189.
[] [PMID: 23196156]
Makni, M.; Sefi, M.; Fetoui, H.; Garoui, M.; Gargouri, N.K.; Boudawara, T.; Zeghal, N. Flax and Pumpkin seeds mixture ameliorates diabetic nephropathy in rats. Food Chem. Toxicol., 2010, 48(8-9), 2407-2412.
[] [PMID: 20570704]
Punithavathi, V.R.; Prince, P.S.M.; Kumar, R.; Selvakumari, J. Antihyperglycaemic, antilipid peroxidative and antioxidant effects of gallic acid on streptozotocin induced diabetic Wistar rats. Eur. J. Pharmacol., 2011, 650(1), 465-471.
[] [PMID: 20863784]
Sefi, M.; Fetoui, H.; Makni, M.; Zeghal, N. Mitigating effects of antioxidant properties of Artemisia campestris leaf extract on hyperlipidemia, advanced glycation end products and oxidative stress in alloxan-induced diabetic rats. Food Chem. Toxicol., 2010, 48(7), 1986-1993.
[] [PMID: 20457207]
Khettaf, A.; Belloula, N.; Dridi, S. Antioxidant activity, phenolic and flavonoid contents of some wild medicinal plants in southeastern Algeria. Afr. J. Biotechnol., 2016, 15, 524-530.
Bouabdelli, F.; Djelloul, A.; Kaid-Omar, Z.; Semmoud, A.; Addou, A. Antimicrobial activity of 22 plants used in urolithiasis medicine in western Algeria. Asian Pac. J. Trop. Dis., 2012, 2, S530-S535.
OCDE Test No. 423: Acute Oral toxicity - Acute Toxic Class Method., 2002.
Sancheti, S.; Sancheti, S.; Bafna, M.; Seo, S-Y. Antihyperglycemic, antihyperlipidemic, and antioxidant effects of Chaenomeles sinensis fruit extract in streptozotocin-induced diabetic rats. Eur. Food Res. Technol., 2010, 231, 415-421.
Manickam, D.; Periyasamy, L. Antidiabetic effect of methanolic extract of Decalepis hamiltonii root (Wight and Arn) in normal and alloxan induced diabetic rats. J. Pharm. Res., 2013, 6, 166-172.
Trinder, P. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Ann. Clin. Biochem., 1969, 6, 24-27.
Rifai, N.; Bachorik, P.S.; Albers, J.J. Lipids, lipoproteins and apolipoproteins.Tietz textbook of clinical chemistry. 3rd ed; WB Saunders Company: Philadelphia, 1999, pp. 809-861.
Ahmed, A.A.; Spaller, M.; Mabry, T.J. Flavonoids of Pallenis spinosa (Asteraceae). Biochem. Syst. Ecol., 1992, 20, 785-786.
Abdel-Wahhab, M.A.; Said, A.; Huefner, A. NMR and radical scavenging activities of patuletin from Urtica urens. Against aflatoxin B1. Pharm. Biol., 2005, 43, 515-525.
Manguro, L.O.A.; Ugi, I.; Lemmen, P. Flavonol Glycosides from the Leaves of Embelia keniensis. J. Chin. Chem. Soc. (Taipei), 2005, 52, 201-208.
Li, M.; Pu, Y.; Yoo, C.G.; Ragauskas, A.J. The occurrence of tricin and its derivatives in plants. Green Chem., 2016, 18, 1439-1454.
Aisyah, L.S.; Yun, Y.F.; Herlina, T.; Julaeha, E.; Zainuddin, A.; Nurfarida, I.; Hidayat, A.T.; Supratman, U.; Shiono, Y. Flavonoid compounds from the leaves of Kalanchoe prolifera and their cytotoxic activity against P-388 murine leukimia cells. Nat. Prod. Sci., 2017, 23, 139-145.
Kumar, S.; Kumar, V.; Prakash, O. Antidiabetic and anti-lipemic effects of Cassia siamea leaves extract in streptozotocin induced diabetic rats. Asian Pac. J. Trop. Med., 2010, 3, 871-873.
Akbarzadeh, A.; Norouzian, D.; Mehrabi, M.R.; Jamshidi, Sh.; Farhangi, A.; Verdi, A.A.; Mofidian, S.M.; Rad, B.L. Induction of diabetes by Streptozotocin in rats. Indian J. Clin. Biochem., 2007, 22(2), 60-64.
[] [PMID: 23105684]
Rakeiten, N. Studies on the diabetogenic action of streptozotocin. Cancer Chemother. Rep., 1963, 29, 91-98.
Scalbert, A.; Manach, C.; Morand, C.; Rémésy, C.; Jiménez, L. Dietary polyphenols and the prevention of diseases. Crit. Rev. Food Sci. Nutr., 2005, 45(4), 287-306.
[] [PMID: 16047496]
Pepato, M.T.; Migliorini, R.H.; Goldberg, A.L.; Kettelhut, I.C. Role of different proteolytic pathways in degradation of muscle protein from streptozotocin-diabetic rats. Am. J. Physiol., 1996, 271(2 Pt 1), E340-E347.
[PMID: 8770029]
Gillespie, K.M. Type 1 diabetes: Pathogenesis and prevention. CMAJ, 2006, 175(2), 165-170.
[] [PMID: 16847277]
Zhang, Y.; Li, X.; Zou, D.; Liu, W.; Yang, J.; Zhu, N.; Huo, L.; Wang, M.; Hong, J.; Wu, P.; Ren, G.; Ning, G. Treatment of type 2 diabetes and dyslipidemia with the natural plant alkaloid berberine. J. Clin. Endocrinol. Metab., 2008, 93(7), 2559-2565.
[] [PMID: 18397984]
Mooradian, A.D. Dyslipidemia in type 2 diabetes mellitus. Nat. Clin. Pract. Endocrinol. Metab., 2009, 5(3), 150-159.
[PMID: 19229235]
Betteridge, D.J. Diabetes, lipoprotein metabolism and atherosclerosis. Br. Med. Bull., 1989, 45(1), 285-311.
[] [PMID: 2676072]
Krishnakumar, K.; Augusti, K.; Vijayammal, P. COMMUNICATIONS-Hypolipidaemic effect of Salacia oblonga wall root bark in streptozotocin diabetic rats. Med. Sci. Res., 2000, 28, 65-68.
Schofield, J.D.; Liu, Y.; Rao-Balakrishna, P.; Malik, R.A.; Soran, H. Diabetes dyslipidemia. Diabetes Ther., 2016, 7(2), 203-219.
[] [PMID: 27056202]
Jain, P.G.; Surana, S.J. Hypolipidemic activity of Prosopis cineraria L (Druce) fruit extract and molecular modeling study with farnesoid X receptor (FXR). Trop. J. Pharm. Res., 2015, 14, 1621-1628.
Ohno, T.; Horio, F.; Tanaka, S.; Terada, M.; Namikawa, T.; Kitoh, J. Fatty liver and hyperlipidemia in IDDM (insulin-dependent diabetes mellitus) of streptozotocin-treated shrews. Life Sci., 2000, 66(2), 125-131.
[] [PMID: 10666008]
Ojieh, A.; Adegor, E.; Ewhre, O.; Enwa, F.; Anachuna, K.; Okunima, A.; Enemose, A.; Miller, O. Antidiabetic, antilipidermic and hepatoprotective effect Of methanolic extract of Cissampelos Owariensis leaves in alloxan induced diabetic rats. J. Int. Res. Med. Pharmaceut. Sci., 2015, 4, 17-28.

Rights & Permissions Print Export Cite as
© 2022 Bentham Science Publishers | Privacy Policy