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Current Bioactive Compounds

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ISSN (Print): 1573-4072
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Research Article

Investigation of Oral Subacute Toxicity and In-Vitro Antioxidant Activity of Standardized Methanolic Extract of Quercus serrata Leaves

Author(s): Maibam B. Chanu, Biseshwori Thongam, Khumukcham Nongalleima, Hans R. Bhat, Surajit K. Ghosh and Anshul Shakya*

Volume 17, Issue 9, 2021

Published on: 24 December, 2020

Article ID: e010621189485 Pages: 8

DOI: 10.2174/1573407216999201224150602

Price: $65

Abstract

Background: Quercus serrata Murray leaves are traditionally used for the treatment of diabetes, dysmenorrhoea, inflammation and urinary tract infection. So, far no study has reported on the toxicological profile and antioxidant properties of the plant.

Objective: The present study aimed to investigate the in-vivo toxicological profile and in-vitro antioxidant activities of the methanolic extract of standardized Quercus serrata leaves.

Methods: Per-oral sub-acute toxicity study was performed in rats using three dose levels (200, 400 and 800 mg/kg b.w.) of the extract for 28 days. The control group received gum acacia suspended in water. Bodyweight was measured weekly. Biochemical parameters were analysed using the serum; the blood-cell count was performed using the whole blood. Pathological changes were also checked in highly perfused tissues. Further, in-vitro reducing power assay, nitric oxide scavenging assay, and DPPH free-radical scavenging assay were performed to evaluate the antioxidant activity of the extract.

Results: There were no significant alterations found in the blood-cell count and biochemical parameters analysed in the treatment group when compared with the normal control. Histopathology study of liver, kidney, pancreas, heart and brain revealed normal cellular architecture in the treatment groups alike the control group animals. Quercus serrata also showed a significant reduction of DPPH with an IC50 value of 4.48±0.254 μg/mL, in-vitro reducing power activity with an IC50 value of 121.65±0.320 μg/mL and nitric oxide scavenging activity with an IC50 value of 106.43±0.338 μg/mL.

Conclusion: The study showed that standardized methanolic extract of Quercus serrata leaves was safe after sub-acute oral administration in rats and possesses good antioxidant potential.

Keywords: Quercus serrata, subacute toxicity, wistar rats, 28 days, in-vitro antioxidant, histopathology.

Graphical Abstract
[1]
Karimi, A.; Majlesi, M.; Rafieian-Kopaei, M. Herbal versus synthetic drugs; beliefs and facts. J. Nephropharmacol., 2015, 4(1), 27-30.
[PMID: 28197471]
[2]
Das, A.; Shakya, A.; Ghosh, S.K.; Singh, U.P.; Bhat, H.R. A Review of phytochemical and pharmacological studies of inula species. Curr. Bioact. Compd., 2020, 16(5), 557-567.
[http://dx.doi.org/10.2174/1573407215666190207093538]
[3]
Shakya, A.; Chatterjee, S.S.; Kumar, V. Holistic psychopharmacology of Fumaria indica (Fumitory). Chin. Med., 2012, 3(4), 182-199.
[http://dx.doi.org/10.4236/cm.2012.34028]
[4]
Shankar, R.; Lavekar, G.S.; Deb, S.; Sharma, B.K. Traditional healing practice and folk medicines used by Mishing community of North East India. J. Ayurveda Integr. Med., 2012, 3(3), 124-129.
[http://dx.doi.org/10.4103/0975-9476.100171] [PMID: 23125508]
[5]
George, P. Concerns regarding the safety and toxicity of medicinal plants - An overview. J. Appl. Pharm. Sci., 2011, 01, 40-44.
[6]
Dolan, L.C.; Matulka, R.A.; Burdock, G.A. Naturally occurring food toxins. Toxins (Basel), 2010, 2(9), 2289-2332.
[http://dx.doi.org/10.3390/toxins2092289] [PMID: 22069686]
[7]
Shakya, A.; Chaudhary, S.K.; Bhat, H.R.; Ghosh, S.K. Acute and sub-chronic toxicity studies of Benincasa hispida (Thunb.) cogniaux fruit extract in rodents. Regul. Toxicol. Pharmacol., 2020, 118, 104785.
[http://dx.doi.org/10.1016/j.yrtph.2020.104785] [PMID: 32976857]
[8]
Zothantluanga, J.H.; Lalnunpuii, H.S.; Bhat, H.R.; Shakya, A. Awareness on the possible adverse effects of Garcinia cambogia: A scientific approach. Sci. Vision., 2019, 19(4), 120-133.
[http://dx.doi.org/10.33493/scivis.19.04.02]
[9]
Paul, A.; Shakya, A.; Zaman, K. Assessment of acute and sub-chronic neurotoxicity of Morus alba L. fruits in rodents. Future J. Pharm. Sci, 2020.
[10]
Farsi, E.; Esmailli, K.; Shafaei, A.; Moradi Khaniabadi, P.; Al Hindi, B.; Khadeer Ahamed, M.B.; Sandai, D.; Abdul Sattar, M.; Ismail, Z.; Abdul Majid, A.M.; Abdul Majid, A.S. Mutagenicity and preclinical safety assessment of the aqueous extract of Clinacanthus nutans leaves. Drug Chem. Toxicol., 2016, 39(4), 461-473.
[http://dx.doi.org/10.3109/01480545.2016.1157810] [PMID: 27033971]
[11]
Albaayit, S.F.A.; Abba, Y.; Abdullah, R.; Abdullah, N. Evaluation of antioxidant activity and acute toxicity of Clausena excavate leaves extract. Evid. Based Complement. Alternat. Med., 2014, 2014, 975450.
[http://dx.doi.org/10.1155/2014/975450] [PMID: 25610488]
[12]
Rahal, A.; Kumar, A.; Singh, V.; Yadav, B.; Tiwari, R.; Chakraborty, S.; Dhama, K. Oxidative stress, prooxidants, and antioxidants: the interplay. BioMed Res. Int., 2014, 2014, 761264.
[http://dx.doi.org/10.1155/2014/761264] [PMID: 24587990]
[13]
Lobo, V.; Patil, A.; Phatak, A.; Chandra, N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn. Rev., 2010, 4(8), 118-126.
[http://dx.doi.org/10.4103/0973-7847.70902] [PMID: 22228951]
[15]
Sheikh, Y.; Maibam, B.C.; Biswas, D.; Laisharm, S.; Deb, L.; Talukdar, N.C.; Borah, J.C. Anti-diabetic potential of selected ethno-medicinal plants of north east India. J. Ethnopharmacol., 2015, 171, 37-41.
[http://dx.doi.org/10.1016/j.jep.2015.05.030] [PMID: 26023028]
[16]
Chanu, M.B.; Labala, R.K.; Sheikh, Y.; Borah, J.C.; Ghosh, S.K.; Sahoo, D.; Shakya, A.; Thongam, B. Bioassay guided isolation of α-glucosidase inhibitory compound, in vivo postprandial anti hyperglycemia and docking study of the isolated compound from the leaves of the methanolic extract of Quercus serrata. Biosci. Biotech. Res. Comm., 2018, 11, 647-657.
[http://dx.doi.org/10.21786/bbrc/11.4/16]
[17]
Ashafa, A.O.T.; Kazeem, M.I. Toxicopathological evaluation of hydroethanol extract of Dianthus basuticus in wistar rats. Evid. Based Complement. Alternat. Med., 2015, 2015, 348519.
[http://dx.doi.org/10.1155/2015/348519] [PMID: 26504473]
[18]
Luna, L.G. Manual of Histologic Staining Methods of the Armed Forces Institute, 3rd; New York, 1968.
[19]
Das, B.; Choudhury, M.D.; Dey, A.; Talukdar, A.D.; Nongalleima, Kh.; Deb, L. Antioxidant and anti-inflammatory activity of aqueous and methanolic extracts of rhizome part of Drynaria quercifolia (L.) J. Smith. Int. J. Pharm. Pharm. Sci., 2014, 6(6), 43-49.
[20]
Gangwar, M.; Gautam, M.K.; Sharma, A.K.; Tripathi, Y.B.; Goel, R.K.; Nath, G. Antioxidant capacity and radical scavenging effect of polyphenol rich Mallotus philippenensis fruit extract on human erythrocytes: an in vitro study. ScientificWorldJournal, 2014, 2014, 279451.
[http://dx.doi.org/10.1155/2014/279451] [PMID: 25525615]
[21]
Sakthivel, G.; Dey, A.; Nongalleima, Kh.; Chavali, M.; Rimal Isaac, R.S.; Singh, N.S.; Deb, L. In vitro and in vivo evaluation of polyhedral formulation against russell’s viper and cobra venom and screening of bioactive components by docking studies. Evid. Based Complement. Alternat. Med., 2013, 2013, 781216.
[http://dx.doi.org/10.1155/2013/781216] [PMID: 23533518]
[22]
Olaniyan, J.M.; Muhammad, H.L.; Makun, H.A.; Busari, M.B.; Abdullah, A.S. Acute and sub-acute toxicity studies of aqueous and methanol extracts of Nelsonia campestris in rats. J. Acute Dis., 2016, 5(1), 62-70.
[http://dx.doi.org/10.1016/j.joad.2015.08.006]
[23]
Phillips, G.B. The lipid composition of serum in patients with liver disease. J. Clin. Invest., 1960, 39, 1639-1650.
[http://dx.doi.org/10.1172/JCI104187] [PMID: 13735297]
[24]
Hasan, K.M.M.; Tamanna, N.; Haque, M.A. Biochemical and histopathological profiling of Wistar rat treated with Brassica napus as a supplementary feed. Food Sci. Hum. Wellness, 2018, 7(1), 77-82.
[http://dx.doi.org/10.1016/j.fshw.2017.12.002]
[25]
Costa-Silva, J.H.; Lima, C.R.; Silva, E.J.R.; Araújo, A.V.; Fraga, M.C.C.A.; Ribeiro E Ribeiro, A.; Arruda, A.C.; Lafayette, S.S.; Wanderley, A.G.; Wanderley, A.G. Acute and subacute toxicity of the Carapa guianensis Aublet (Meliaceae) seed oil. J. Ethnopharmacol., 2008, 116(3), 495-500.
[http://dx.doi.org/10.1016/j.jep.2007.12.016] [PMID: 18281172]
[26]
Reddy, K.S.; Sudheer, A.; Pradeepkumar, B.; Reddy, C.S. Effect of a polyherbal formulation in streptozotocin-induced diabetic nephropathy in wistar rats. Indian J. Pharmacol., 2019, 51(5), 330-336.
[http://dx.doi.org/10.4103/ijp.IJP_217_18] [PMID: 31831922]
[27]
Loha, M.; Mulu, A.; Abay, S.M.; Ergete, W.; Geleta, B. Acute and subacute toxicity of methanol extract of Syzygium guineense leaves on the histology of the liver and kidney and biochemical compositions of blood in rats. Evid. Based Complement. Alternat. Med., 2019, 2019, 5702159.
[http://dx.doi.org/10.1155/2019/5702159] [PMID: 30956682]
[28]
Shao, L.; Wang, Y.; Chang, J.; Luo, Y.; Meng, A.; Zhou, D. Hematopoietic stem cell senescence and cancer therapy-induced long-term bone marrow injury. Transl. Cancer Res., 2013, 2(5), 397-411.
[PMID: 24605290]
[29]
Sahota, T.; Danhof, M.; Della Pasqua, O. Pharmacology-based toxicity assessment: towards quantitative risk prediction in humans. Mutagenesis, 2016, 31(3), 359-374.
[http://dx.doi.org/10.1093/mutage/gev081] [PMID: 26970519]
[30]
Zheleva-Dimitrova, D.; Simeonova, R.; Gevrenova, R.; Savov, Y.; Balabanova, V.; Nasar-Eddin, G.; Bardarov, K.; Danchev, N. In vivo toxicity assessment of Clinopodium vulgare L. water extract characterized by UHPLC-HRMS. Food Chem. Toxicol., 2019, 134, 110841.
[http://dx.doi.org/10.1016/j.fct.2019.110841] [PMID: 31568851]
[31]
Clemente, M.; Miguel, M.D.; Felipe, K.B.; Gribner, C.; Moura, P.F.; Rigoni, A.G.R.; Fernandes, L.C.; Carvalho, J.L.S.; Hartmann, I.; Piltz, M.T.; Henneberg, R.; Montrucchio, D.P.; Miguel, O.G. Acute and sub-acute oral toxicity studies of standardized extract of Nasturtium officinale in Wistar rats. Regul. Toxicol. Pharmacol., 2019, 108, 104443.
[http://dx.doi.org/10.1016/j.yrtph.2019.104443] [PMID: 31437473]
[32]
Abid, R.; Mahmood, R. Acute and sub-acute oral toxicity of ethanol extract of Cassia fistula fruit in male rats. Avicenna J. Phytomed., 2019, 9(2), 117-125.
[PMID: 30984576]
[33]
Bhalodia, N.R.; Nariya, P.B.; Acharya, R.N.; Shukla, V.J. In vitro antioxidant activity of hydro alcoholic extract from the fruit pulp of Cassia fistula Linn. Ayu, 2013, 34(2), 209-214.
[http://dx.doi.org/10.4103/0974-8520.119684] [PMID: 24250133]
[34]
Joshi, A.; Prasad, S.K.; Joshi, V.K.; Hemalatha, S. Phytochemical standardization, antioxidant, and antibacterial evaluations of Leea macrophylla: A wild edible plant. Yao Wu Shi Pin Fen Xi, 2016, 24(2), 324-331.
[http://dx.doi.org/10.1016/j.jfda.2015.10.010] [PMID: 28911586]
[35]
Bloodsworth, A.; O’Donnell, V.B.; Freeman, B.A. Nitric oxide regulation of free radical- and enzyme-mediated lipid and lipoprotein oxidation. Arterioscler. Thromb. Vasc. Biol., 2000, 20(7), 1707-1715.
[http://dx.doi.org/10.1161/01.ATV.20.7.1707] [PMID: 10894807]
[36]
Scicinski, J.; Oronsky, B.; Ning, S.; Knox, S.; Peehl, D.; Kim, M.M.; Langecker, P.; Fanger, G. NO to cancer: The complex and multifaceted role of nitric oxide and the epigenetic nitric oxide donor, RRx-001. Redox Biol., 2015, 6, 1-8.
[http://dx.doi.org/10.1016/j.redox.2015.07.002] [PMID: 26164533]
[37]
Gorinstein, S.; Haruenkit, R.; Poovarodom, S.; Vearasilp, S.; Ruamsuke, P.; Namiesnik, J.; Leontowicz, M.; Leontowicz, H.; Suhaj, M.; Sheng, G.P. Some analytical assays for the determination of bioactivity of exotic fruits. Phytochem. Anal., 2010, 21(4), 355-362.
[http://dx.doi.org/10.1002/pca.1207] [PMID: 20183860]

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