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Current Nanoscience


ISSN (Print): 1573-4137
ISSN (Online): 1875-6786

General Research Article

Iron Oxide Nanoparticles Synthesized Via Green Tea Extract for Doxorubicin Delivery

Author(s): Lei Nie*, Chenlei Cai, Meng Sun, Fang Zhang, Lingyun Zheng, Qi Peng, Amin Shavandi* and Shoufeng Yang

Volume 17, Issue 4, 2021

Published on: 29 October, 2020

Page: [646 - 657] Pages: 12

DOI: 10.2174/1573413716999201029205654

open access plus


Background: Due to the limitation of conventional cancer treatment using chemotherapy, the nanoparticle therapeutics have shown enhanced efficacy with alleviating side effects.

Objective: The aim of this study was to prepare the superparamagnetic iron oxide nanoparticles (TC- SPION) for doxorubicin (DOX) loading and delivery.

Methods: Here, we reported a simple green strategy to fabricate T-C-SPION using green tea extract and citric acid. Also, the anti-cancer drug, DOX, was used as a model drug to fabricate DOX-loaded nanoparticles.

Results: The formed T-C-SPION nanoparticles were spherical with a diameter of 23.8 ± 0.8 nm, as confirmed by Transmission Electron Microscopy (TEM). Besides, Dynamic Light Scattering (DLS) revealed that the prepared nanoparticles were water-dispersible and stable while stored in water for 6 weeks. The CCK-8 assay showed T-C-SPION to have a good cytocompatibility using different iron concentrations (10 ~ 120 ug/mL). Furthermore, T-C-SPION had a higher DOX encapsulation efficiency (Eencaps), around 43.2 ± 1.8 %, which resulted in a lagged release profile of DOX, compared to other types of iron oxide nanoparticles using green tea or citric acid alone. Next, cell viability assay indicated that T-C-SPION with a higher Eencaps showed superior and sustained cytotoxicity compared to the control group.

Conclusion: The developed iron oxide nanoparticles synthesized by green tea extract and citric acid in this paper could be considered as a potential drug carrier for cancer therapy applications.

Keywords: Nanoparticles, iron oxide, green tea, citric acid, doxorubicin, drug delivery, cytocompatibility.

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