Generic placeholder image

Current Analytical Chemistry

Editor-in-Chief

ISSN (Print): 1573-4110
ISSN (Online): 1875-6727

General Research Article

Novel Reverse-Phase High-Performance Liquid Chromatography (RPHPLC) Method for the Quantification of Apigenin in Ocimum Basilicum Linn Seeds (Tukmaria)

Author(s): Nisha H. Parikh and Charmy Kothari*

Volume 17, Issue 4, 2021

Published on: 26 October, 2020

Page: [528 - 535] Pages: 8

DOI: 10.2174/1573411016999201026192955

Price: $65

Abstract

Background: Ocimum basilicum L. seeds, is commonly also known as Takhmaria in Gujarat. It is a common constituent present in fruits, plant-derived beverages, vegetables, wheat, sprouts, and some seasonings.

Objective: A simple, specific, precise, accurate, and sensitive method for the quantification of apigenin by reverse-phase high-performance liquid chromatography (RP-HPLC) was developed and validated.

Methods: Analysis was carried out on Inertsil ODS-3V-C18 column (250 mm × 4.6 mm i.d, 5 μm) as stationary phase and methanol-acetonitrile (55:45 v/v) as a mobile phase at a flow rate of 1.0 mL/ min. Detection was carried out at 340 nm. The retention time of apigenin was found to be 8.30 min. The proposed method was validated according to ICH Guidelines, Q2 (R1).

Results: The developed method showed good linearity in the range of 10-50 μg/mL with a correlation coefficient (R2= 0.9998). The LOD and LOQ were found to be 1.23 μg/mL and 4.05 μg/mL, respectively. The percentage recovery for apigenin was found to be 97.75-100.5%. All validation parameters were found within acceptable limits and demonstrated good reliability in the quantification of apigenin.

Conclusion: Thus, the newly developed and validated method can be successfully applied for the quantification of apigenin from seeds of O. basilicum L. and can also be applied for the standardization of polyherbal formulation containing O. basilicum seeds. Assay results showed good recovery when statistically compared with the high-performance thin-layer chromatography (HPTLC) method.

Keywords: Apigenin, ocimum basilicum seeds, tukmaria, rp-hplc, methanolic extract, quantification.

Graphical Abstract
[1]
Budavari, S. The Merck Index An Encyclopedia of Chemicals, 13th ed; Whitehouse Station, NJ, 2006, pp. 123-124.
[2]
McKay, D.L.; Blumberg, J.B. A review of the bioactivity and potential health benefits of chamomile tea (Matricaria recutita L.). Phytother. Res., 2006, 20(7), 519-530.
[http://dx.doi.org/10.1002/ptr.1900] [PMID: 16628544]
[3]
Patel, D.; Shukla, S.; Gupta, S. Apigenin and cancer chemoprevention: Progress, potential and promise. (review) Int. J. Oncol., 2007, 30(1), 233-245.
[http://dx.doi.org/10.3892/ijo.30.1.233] [PMID: 17143534]
[4]
Shukla, S.; Gupta, S. Apigenin: A promising molecule for cancer prevention. Pharm. Res., 2010, 27(6), 962-978.
[http://dx.doi.org/10.1007/s11095-010-0089-7] [PMID: 20306120]
[5]
Bevilacqua, L.; Buiarelli, F.; Coccioli, F.; Jasionowska, R. Identification of compounds in wine by HPLC-tandem mass spectrometry. Ann. Chim., 2004, 94(9-10), 679-689.
[http://dx.doi.org/10.1002/adic.200490085] [PMID: 15506618]
[6]
Gerhäuser, C. Beer constituents as potential cancer chemopreventive agents. Eur. J. Cancer, 2005, 41(13), 1941-1954.
[http://dx.doi.org/10.1016/j.ejca.2005.04.012] [PMID: 15953717]
[7]
Yang, C.S.; Landau, J.M.; Huang, M.T.; Newmark, H.L. Inhibition of carcinogenesis by dietary polyphenolic compounds. Annu. Rev. Nutr., 2001, 21, 381-406.
[http://dx.doi.org/10.1146/annurev.nutr.21.1.381] [PMID: 11375442]
[8]
Amable, T.G. In Vitro antimicrobial efficacy of fractions from onion (Allium Cepa) leaves extract from Wukro, Ethiopia. J. Nat. Prod., 2015, 3(5), 365-368.
[9]
Benedec, D.; Pârvu, A.E.; Oniga, I.; Toiu, A.; Tiperciuc, B. Effects of Ocimum basilicum L. extract on experimental acute inflammation. Rev. Med. Chir. Soc. Med. Nat. Iasi, 2007, 111(4), 1065-1069.
[PMID: 18389806]
[10]
Falowo, A.B.; Mukumbo, F.E.; Idamokoro, E.M.; Afolayan, A.J.; Muchenje, V. Phytochemical constituents and antioxidant activity of sweet basil (Ocimum basilicum L.) essential oil on ground beef from boran and nguni cattle. Int. J. Food Sci., 2019.20192628747
[http://dx.doi.org/10.1155/2019/2628747] [PMID: 30713849]
[11]
Singh, S. Evaluation of gastric anti-ulcer activity of fixed oil of Ocimum basilicum Linn. and its possible mechanism of action. Indian J. Exp. Biol., 1999, 37(3), 253-257.
[PMID: 10641155]
[12]
Muralidharan, A.; Dhananjayan, R. The Cardiac stimulant activity of Ocimum basilicum Linn. Extracts. Indian J. Pharmacol., 2004, 36(3), 163-166.
[13]
Dasgupta, T.; Rao, A.R.; Yadava, P.K. Chemomodulatory efficacy of basil leaf (Ocimum basilicum) on drug metabolizing and antioxidant enzymes, and on carcinogen-induced skin and forestomach papillomagenesis. Phytomedicine, 2004, 11(2-3), 139-151.
[http://dx.doi.org/10.1078/0944-7113-00289] [PMID: 15070164]
[14]
Meera, R.; Devi, P.; Kameswari, B.; Madhumitha, B.; Merlin, N.J. Antioxidant and hepatoprotective activities of Ocimum basilicum Linn. and Trigonella foenum-graecum Linn. against H2O2 and CCL4 induced hepatotoxicity in goat liver. Indian J. Exp. Biol., 2009, 47(7), 584-590.
[PMID: 19761043]
[15]
Zeggwagh, N.A.; Sulpice, T.; Eddouks, M.; Pope, U. Anti-hyperglycaemic and hypolipidemic effects of ocimum basilicum aqueous extract in diabetic rats. Am. J. Pharmacol. Toxicol., 2007, 2(3), 123-129.
[http://dx.doi.org/10.3844/ajptsp.2007.123.129]
[16]
Jayaweera, D. Medicinal plants (Indigenous and Exotic) Used in Ceylon Part III, Colombo , 1981; pp. 101-103.
[17]
Purushothaman, B.; Srinivasan, R.P.; Suganthi, P.; Ranganathan, V.; Gimbun, J.; Shanmugam, K. A Comprehensive review on Ocimum basilicum. J. Nat. Rem., 2018, 18(3), 71-85.
[http://dx.doi.org/10.18311/jnr/2018/21324]
[18]
Abd El-Ghffar, E.A.; Al-Sayed, E.; Shehata, S.M.; Eldahshan, O.A.; Efferth, T. The protective role of Ocimum basilicum L. (Basil) against aspirin-induced gastric ulcer in mice: Impact on oxidative stress, inflammation, motor deficits and anxiety-like behavior. Food Funct., 2018, 9(8), 4457-4468.
[http://dx.doi.org/10.1039/C8FO00538A] [PMID: 30073231]
[19]
Abdullah, I.H.; Farooq, A.; Syed, T.H. Roman, Przyb. Chemical Composition, antioxidant and antimicrobial activities of basil (Ocimum Basilicum) essential oils. Food Chem., 2008, 108(3), 986-995.
[http://dx.doi.org/10.1016/j.foodchem.2007.12.010] [PMID: 26065762]
[20]
Anjaneyalu, Y.V.; Gowda, D.C. structural studies of an acidic polysaccharide from ocimum basilicum seeds. Carbohydr. Res., 1979, 75, 251-256.
[http://dx.doi.org/10.1016/S0008-6215(00)84644-3]
[21]
Parikh, N.H.; Kothari, C.S. Phytochemical analysis and total phenolic and flavonoid contents determination of methanolic extract of ocimum basilicum l seed. Int. J. Pharm. Tech. Res., 2016, 9(4), 215-219.
[22]
Parikh, N.H.; Kothari, C.S. Therapeutic role of methanolic extract of ocimum basilicum l. seeds and its isolated compound as potent antidiabetic and antihyperlipidemic agents. J. Nat. Prod., 2019, 9, 1-10.
[http://dx.doi.org/10.2174/2210315509666190117145617]
[23]
Gudzenko, A. Development and validation of a RP-HPLC method for the simultaneous determination of luteolin and apigenin in herb of achillea millefolium. Pharma Innovation, 2013, 7(2), 7-14.
[24]
Parikh, N.H.; Kothari, C.S. Development and validation of a high-performance thin-layer chromatographic – densitometric method for the quantification of apigenin in ocimum basilicum. J Planer Chromat, 2016, 29(3), 216-220.
[http://dx.doi.org/10.1556/1006.2016.29.3.8]
[25]
International Conference on Harmonization (ICH) Validation of Analytical Procedures: Text and Methodology Q2 (R1) 2005.

Rights & Permissions Print Cite
© 2024 Bentham Science Publishers | Privacy Policy