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Current Catalysis


ISSN (Print): 2211-5447
ISSN (Online): 2211-5455

Research Article

Alteration of Activation Energy of a Reaction in the Catalase of Human Erythrocyte by Cimetidine, an In vitro Thermokinetic Study

Author(s): Dariush Minai-Tehrani*

Volume 9, Issue 2, 2020

Page: [148 - 151] Pages: 4

DOI: 10.2174/2211544709999201023145901

Price: $65


Background: Hydrogen peroxide is normally formed during the metabolic pathway of the body. It is a toxic compound for vital cells, which can oxidize many macromolecules and cause damage in cells. Catalase can degrade H2O2 in cells and prevent cell injury. Cimetidine is a histamine H2 receptor blocker which decreases the release of stomach acid and is used for gastrointestinal diseases. Cimetidine inhibited catalase by mixed inhibition.

Objective: In this study, the effect of temperature on the binding of cimetidine to human erythrocyte catalase was investigated and kinetic factors of the binding were determined.

Results: Dixon plot confirmed the mixed type of inhibition and determined the Ki of the drug. The maximum activity of the enzyme was observed at 30°C. Arrhenius plot demonstrated that the activation energy of the enzyme reaction in the absence and presence of cimetidine was about 4.7 and 8.13 kJ/mol, respectively. The temperature coefficient (Q30-40) was determined as about 1.11 and 1.09 in the absence and presence of cimetidine.

Conclusion: Cimetidine was able to increase the activation energy of the reaction of catalase, which confirmed the inhibition of the enzyme based on the kinetic results.

Keywords: Activation energy, enzyme, kinetics, erythrocyte, inhibition, cimetidine.

Graphical Abstract
Góth L, Rass P, Páy A. Catalase enzyme mutations and their association with diseases. Mol Diagn 2004; 8(3): 141-9.
[] [PMID: 15771551]
Goyal MM, Basak A. Hydroxyl radical generation theory: a possible explanation of unexplained actions of mammalian catalase. Int J Biochem Mol Biol 2012; 3(3): 282-9.
[] [PMID: 23097744]
Sumner JB, Dounce AL. Crystalline catalase. Science 1937; 85(2206): 366-7.
[] [PMID: 17776781]
Storz G, Imlay JA. Oxidative stress. Curr Opin Microbiol 1999; 2(2): 188-94.
[] [PMID: 10322176]
Brioukhanov AL, Netrusov AI, Eggen RIL. The catalase and superoxide dismutase genes are transcriptionally up-regulated upon oxidative stress in the strictly anaerobic archaeon Methanosarcina barkeri. Microbiology (Reading) 2006; 152(Pt 6): 1671-7.
[] [PMID: 16735730]
Wolfe AD, Beers RF Jr, Sizer IW. Cyanide inhibition of the catalase system. Arch Biochem Biophys 1957; 72(2): 353-7.
[] [PMID: 13479119]
Aksoy Y, Balk M, Ogus H, Ozer N. The mechanism of inhibition of human erythrocyte catalase by azide. Turk J Biol 2014; 28: 65-70.
Casalino E, Calzaretti G, Sblano C, Landriscina C. Molecular inhibitory mechanisms of antioxidant enzymes in rat liver and kidney by cadmium. Toxicology 2002; 179(1-2): 37-50.
[] [PMID: 12204541]
Clark WG, Brater DC, Johnson AR. Goth’s medical pharmacology. 12th ed. USA: Mosby Company 1988; pp. 210-3.
Bodemar G, Walan A. Cimetidine in the treatment of active duodenal and prepyloric ulcers. Lancet 1976; 2(7978): 161-4.
[] [PMID: 73792]
Jensen RT, Collen MJ, McArthur KE, et al. Comparison of the effectiveness of ranitidine and cimetidine in inhibiting acid secretion in patients with gastric hypersecretory states. Am J Med 1984; 77(5B): 90-105.
[PMID: 6150641]
Palm K, Luthman K, Ros J, Grasjo J, Artursson P. Effect of molecular charge on intestinal epithelial drug transport: pH-dependent transport of cationic drugs. J Pharmacol Exp Ther 1999; 291(2): 435-43.
[PMID: 10525056]
Yazdi F, Minai-Tehrani D, Jahngirvand M, et al. Functional and structural changes of human erythrocyte catalase induced by cimetidine: proposed model of binding. Mol Cell Biochem 2015; 404(1-2): 97-102.
[] [PMID: 25739358]
Masoud M, Ebrahimi F, Minai-Tehrani D. Effect of cimetidine on catalase activity of Pseudomonas aeruginosa: a suggested mechanism of action. J Mol Microbiol Biotechnol 2014; 24(3): 196-201.
[] [PMID: 24993120]
Goyal MM, Basak A. Human catalase: looking for complete identity. Protein Cell 2010; 1(10): 888-97.
[] [PMID: 21204015]
Altikat S, Coban A, Ciftci M, Ozdemir H. In vitro effects of some drugs on catalase purified from human skin. J Enzyme Inhib Med Chem 2006; 21(2): 231-4.
[] [PMID: 16789438]
Minai-Tehrani D, Ashrafi IS, Mohammadi MK, Damavandifar ZS, Zonouz ER, Pirshahed TE. Comparing inhibitory effect of tramadol on catalase of Pseudomonas aeruginosa and mouse liver. Curr Enzym Inhib 2014; 10: 54-8.

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