Generic placeholder image

Current Pharmaceutical Biotechnology

Editor-in-Chief

ISSN (Print): 1389-2010
ISSN (Online): 1873-4316

Research Article

Oral Administration of Aloe vera Ameliorates Wound Healing through Improved Angiogenesis and chemotaxis in Sprague Dawley Rats

Author(s): Fatima Ali, Nadia Wajid*, Maryam G. Sarwar and Aamer M. Qazi

Volume 22, Issue 8, 2021

Published on: 01 October, 2020

Page: [1122 - 1128] Pages: 7

DOI: 10.2174/1389201021999201001204345

Price: $65

Abstract

Background: Aloe vera has been reported as a topical antibiotic and healing agent for wounds, but advantages of oral administration and mechanisms of wound healing have not been reported. Present study focuses on the evaluation of effects of oral administration of Aloe vera for excisional cutaneous wounds in Sprague Dawley rats.

Methods: Sprague Dawley (SD) rats were inflicted with excisional wounds and were either treated with Aloe vera orally (Aloe vera) or kept untreated (wound). In contrast, healthy rats were kept as control group. Wound area was measured from day 7th to day 21st. Collagen content was estimated by hydroxyproline assay. Histology was analysed by hematoxylin and eosin staining. Angiogenesis was observed by indirect ELISA for Insulin like Growth Factor (IGF-1) and Vascular Endothelial Growth Factor (VEGF) protein from skin, serum and bone marrow. Chemotaxis was evaluated by RT-PCR analysis for Stromal cell-Derived Factor-1 (SDF-1) and C-X-C chemokine receptor type 4 (CXCR-4) from skin and bone marrow.

Results: Aloe vera healed wounds earlier than untreated rats with gradual improvement in wound areas and collagen content. Aloe vera also improved the expression of IGF-1 and VEGF in skin and bone marrow indicating an improvement in angiogenesis. RT- PCR analysis showed increased expression of genes for chemotaxis (SDF-1 and CXCR-4) in skin and bone marrow.

Conclusion: Aloe vera improves healing by increasing collagen content, improving angiogenesis and chemotaxis.

Keywords: Wounds, Aloe vera, skin, angiogenesis, hydroxyproline, herbs.

Graphical Abstract
[1]
Gebremeskel, L. In vivo wound healing and anti-inflammatory activities of leaf latex of Aloe megalacantha Baker (Xanthorrhoeaceae). Evidence-based Complement. Altern. Med., 2018, Article ID 5037912..
[2]
Lanci, A.; Merlo, B.; Mariella, J.; Castagnetti, C.; Iacono, E. Heterologous Wharton’s jelly derived mesenchymal stem cells application on a large chronic skin wound in a 6-month-old filly. Front. Vet. Sci., 2019, 6, 9.
[http://dx.doi.org/10.3389/fvets.2019.00009] [PMID: 30761313]
[3]
Hussan, F.; Yahaya, M.F.; Teoh, S.L.; Das, S. Herbs for effective treatment of diabetes mellitus: Medicinal chemistry and future therapeutic options. Mini Rev. Med. Chem., 2018, 18(8), 697-710.
[PMID: 28971772]
[4]
Premoli, M.; Aria, F.; Bonini, S.A.; Maccarinelli, G.; Gianoncelli, A.; Pina, S.D.; Tambaro, S.; Memo, M.; Mastinu, A. Cannabidiol: Recent advances and new insights for neuropsychiatric disorders treatment. Life Sci., 2019, 224, 120-127.
[http://dx.doi.org/10.1016/j.lfs.2019.03.053] [PMID: 30910646]
[5]
Adams, K.; Eliot, T.; Gerald, A. Extent of use of Aloe vera m,s of Kitagata sub-county in Sheema District, Western Uganda. Int. J. Sci. Basic Appl. Res., 2014, 15(1), 1-15.
[PMID: 26855960]
[6]
Kumar, A.; Premoli, M.; Aria, F.; Bonini, S.A.; Maccarinelli, G.; Gianoncelli, A.; Memo, M.; Mastinu, A. Cannabimimetic plants: Are they new cannabinoidergic modulators? Planta, 2019, 249(6), 1681-1694.
[http://dx.doi.org/10.1007/s00425-019-03138-x] [PMID: 30877436]
[7]
Bhattarai, N.K. Traditional herbal medicines used to treat wounds and injuries in Nepal. Trop. Doct., 1997, 27(Suppl. 1), 43-47.
[http://dx.doi.org/10.1177/00494755970270S114] [PMID: 9204725]
[8]
Jarić, S.; Kostić, O.; Mataruga, Z.; Pavlović, D.; Pavlović, M.; Mitrović, M.; Pavlović, P. Traditional wound-healing plants used in the Balkan region (Southeast Europe). J. Ethnopharmacol., 2018, 211, 311-328.
[http://dx.doi.org/10.1016/j.jep.2017.09.018] [PMID: 28942136]
[9]
Khan, A.W.; Kotta, S.; Ansari, S.H.; Sharma, R.K.; Kumar, A.; Ali, J. Formulation development, optimization and evaluation of Aloe vera gel for wound healing. Pharmacogn. Mag., 2013, 9(Suppl. 1), S6-S10.
[http://dx.doi.org/10.4103/0973-1296.117849] [PMID: 24143047]
[10]
Liu, C.; Cui, Y.; Pi, F.; Cheng, Y.; Guo, Y.; Qian, H. Extraction, purification, structural characteristics, biological activities and pharmacological applications of acemannan, a polysaccharide from Aloe vera: A review. Molecules, 2019, 24(8), 1554.
[http://dx.doi.org/10.3390/molecules24081554] [PMID: 31010204]
[11]
Medellín-Luna, M.F.; Castañeda-Delgado, J.E.; Martínez-Balderas, V.Y.; Cervantes-Villagrana, A.R. Medicinal plant extracts and their use as wound closure inducing agents. J. Med. Food, 2019, 22(5), 435-443.
[http://dx.doi.org/10.1089/jmf.2018.0145] [PMID: 30942656]
[12]
Pereira, G.G.; Guterres, S.S.; Balducci, A.G.; Colombo, P.; Sonvico, F. Polymeric films loaded with vitamin E and Aloe vera for topical application in the treatment of burn wounds. Biomed Res. Int., 2014, 2014, 641590.
[13]
Kumar, R.; Singh, A.K.; Gupta, A.; Bishayee, A.; Pandey, A.K. Therapeutic potential of Aloe vera-A miracle gift of nature. Phytomedicine, 2019, 60, 152996.
[http://dx.doi.org/10.1016/j.phymed.2019.152996] [PMID: 31272819]
[14]
Palmieri, B.; Vadalà, M.; Laurino, C. Nutrition in wound healing: investigation of the molecular mechanisms, a narrative review. J. Wound Care, 2019, 28(10), 683-693.
[http://dx.doi.org/10.12968/jowc.2019.28.10.683] [PMID: 31600106]
[15]
Anuszewska, E.L. Mechanisms of therapeutic action of aloe. Wiad. Lek., 2015, 68(2), 168-172.
[PMID: 26181152]
[16]
Mackay, D.; Miller, A.L. Nutritional support for wound healing. Altern. Med. Rev., 2003, 8(4), 359-377.
[17]
Sorg, H.; Tilkorn, D.J.; Hager, S.; Hauser, J.; Mirastschijski, U. Skin wound healing: An update on the current knowledge and concepts. Eur. Surg. Res., 2017, 58(1-2), 81-94.
[http://dx.doi.org/10.1159/000454919] [PMID: 27974711]
[18]
Muniandy, K.; Gothai, S.; Arulselvan, P.; Kumar, S.S.; Norhaizan, M.E.; Umamaheswari, A.; Fakurazi, S. Mini review: Wound healing potential of edible plants. Pak. J. Pharm. Sci., 2019, 32(2), 703-707.
[PMID: 31081786]
[19]
Branski, L.K.; Gauglitz, G.G.; Herndon, D.N.; Jeschke, M.G. A review of gene and stem cell therapy in cutaneous wound healing. Burns, 2009, 35(2), 171-180.
[http://dx.doi.org/10.1016/j.burns.2008.03.009] [PMID: 18603379]
[20]
Deng, Z.H.; Yin, J.J.; Luo, W.; Kotian, R.N.; Gao, S.S.; Yi, Z.Q.; Xiao, W.F.; Li, W.P.; Li, Y.S. The effect of earthworm extract on promoting skin wound healing. Biosci. Rep., 2018, 38(2), BSR20171366.
[http://dx.doi.org/10.1042/BSR20171366] [PMID: 29531018]
[21]
de Mayo, T.; Conget, P.; Becerra-Bayona, S.; Sossa, C.L.; Galvis, V.; Arango-Rodríguez, M.L. The role of bone marrow mesenchymal stromal cell derivatives in skin wound healing in diabetic mice. PLoS One, 2017, 12(6), e0177533.
[http://dx.doi.org/10.1371/journal.pone.0177533] [PMID: 28594903]
[22]
Balaji, S.; LeSaint, M.; Bhattacharya, S.S.; Moles, C.; Dhamija, Y.; Kidd, M.; Le, L.D.; King, A.; Shaaban, A.; Crombleholme, T.M.; Bollyky, P.; Keswani, S.G. Adenoviral-mediated gene transfer of insulin-like growth factor 1 enhances wound healing and induces angiogenesis. J. Surg. Res., 2014, 190(1), 367-377.
[http://dx.doi.org/10.1016/j.jss.2014.02.051] [PMID: 24725678]
[23]
Chen, L.; Tredget, E.E.; Wu, P.Y.G.; Wu, Y.; Wu, Y. Paracrine factors of mesenchymal stem cells recruit macrophages and endothelial lineage cells and enhance wound healing. PLoS One, 2008, 3(4), e1886.
[http://dx.doi.org/10.1371/journal.pone.0001886] [PMID: 18382669]
[24]
Shahouzehi, B.; Sepehri, G.; Sadeghiyan, S.; Masoomi-Ardakani, Y. Effect of Pistacia atlantica resin oil on anti-oxidant, hydroxyprolin and VEGF changes in experimentally-induced skin burn in rat. World J. Plast. Surg., 2018, 7(3), 357-363.
[http://dx.doi.org/10.29252/wjps.7.3.357] [PMID: 30560077]
[25]
Tang, T.; Jiang, H.; Yu, Y.; He, F.; Ji, S.Z.; Liu, Y.Y.; Wang, Z.S.; Xiao, S.C.; Tang, C.; Wang, G.Y.; Xia, Z.F. A new method of wound treatment: Targeted therapy of skin wounds with reactive oxygen species-responsive nanoparticles containing SDF-1α. Int. J. Nanomedicine, 2015, 10, 6571-6585.
[PMID: 26527874]

Rights & Permissions Print Export Cite as
© 2022 Bentham Science Publishers | Privacy Policy