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Current Pharmaceutical Biotechnology

Editor-in-Chief

ISSN (Print): 1389-2010
ISSN (Online): 1873-4316

Mini-Review Article

Recent Progress in Saikosaponin Biosynthesis in Bupleurum

Author(s): Chun Sui, Wen-Jing Han, Chu-Ran Zhu and Jian-He Wei*

Volume 22, Issue 3, 2021

Published on: 18 September, 2020

Page: [329 - 340] Pages: 12

DOI: 10.2174/1389201021999200918101248

Price: $65

Abstract

Background: Chaihu is a popular traditional Chinese medicine that has been used for centuries. It is traditionally used to treat cold fever and liver-related diseases. Saikosaponins (SSs) are one of the main active components of chaihu, in addition to essential oils, flavonoids, and polysaccharides. Considerable effort is needed to reveal the biosynthesis and regulation of SSs on the basis of current progress.

Objective: The aim of this study is to provide a reference for further studies and arouse attention by summarizing the recent achievements of SS biosynthesis.

Methods: All the data compiled and presented here were obtained from various online resources, such as PubMed Scopus and Baidu Scholar in Chinese, up to October 2019.

Results: A few genes of the enzymes of SSs participating in the biosynthesis of SSs were isolated. Among these genes, only the P450 gene was verified to catalyze the SS skeleton β-amyrin synthase. Several UDP-glycosyltransferase genes were predicted to be involved in the biosynthesis of SSs. SSs could be largely biosynthesized in the phloem and then transported from the protoplasm, which is the biosynthetic site, to the vacuoles to avoid self-poisoning. As for the other secondary metabolites, the biosynthesis of SSs was strongly affected by environmental factors and the different species belonging to the genus of Bupleurum. Transcriptional regulation was studied at the molecular level.

Conclusion: Profound discoveries in SSs may elucidate the mechanism of diverse the monomer formation of SSs and provide a reference for maintaining the stability of SS content in Radix Bupleuri.

Keywords: Bupleurum, saikosaponin, biosynthesis, transcriptional regulation, stress response, vacuoles.

Graphical Abstract
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