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Current Biochemical Engineering (Discontinued)


ISSN (Print): 2212-7119
ISSN (Online): 2212-7127

Research Article

Effect of Choline Acetate on the Yeast Cells During Fermentation: Kinetics Approach

Author(s): Amal A. Elgharbawy*, Md. Zahangir Alam, Muhammad Moniruzzaman, Nassereldeen A. Kabbashi, Parveen Jamal and Shiva R. Motlagh

Volume 6 , Issue 3 , 2020

Page: [172 - 179] Pages: 8

DOI: 10.2174/2212711906999200816125102

Price: $65


Background: Ionic liquids (ILs) are claimed as green solvents and have demonstrated ability in the dissolution of several biomaterials and polymers, thus promoting their applications in wide scope. The aim of this study was to evaluate the yeast cells in the ionic liquid (IL), choline acetate [Cho]OAc as an eco-friendlier IL for their growth and ability of fermentation of the empty fruit bunch (EFB) biomass for ethanol production via monitoring the cell growth curve and kinetics.

Methods: The Saccharomyces cerevisiae was cultured for five days on yeast extract-peptone-dextrose (YPD) agar and the sub-culturing was carried out every two weeks to evaluate the growth of yeast in [Cho]OAc at different concentrations; 5%, 10%, and 20%, where it was pre-cultivated for 24 h at 30 °C and 150 rpm in a shaking incubator as a control culture. Yeast cell number was determined by counting using a hemocytometer. Also, the kinetic growth of yeast during the fermentation of EFB was evaluated.

Result: Cell concentration increased, ethanol production increased along with the optical density with the peak reached at 72 h, then declined. Enzymatic hydrolysis process followed by fermentation of EFB using [Cho][OAc] improved the yield of sugar, saccharification and ethanol around 1.938, 2.879, and 2.165 times in comparison with untreated EFB with IL. Furthermore, values of specific growth rates obtained in IL-medium and non-IL-medium are applicable for ethanol production to take place. The results showed the ability of yeast cells for fermentation even in the presence of IL.

Conclusion: The results showed that the presence of the IL did not suppress the growth of the yeast cells and did not impair the fermentation productivity.

Keywords: Ionic liquids, fermentation, hydrolysis, cellulase, kinetics, growth curve.

Graphical Abstract
N. Jaqueline, M. Ramos, S.J. Crystyne, S. Manuella, and M.M.M. Alves, M. da G. Nascimento, J.M.R.J.C. da Silva, J.M.R.J.C. da Silva, M.M.M. Alves, "The use of organic solvents/ionic liquids mixtures in reactions catalyzed by lipase from Burkholderia cepacia immobilized in different supports", J. Mol. Catal., B Enzym., vol. 112, pp. 1-8, 2015..
M. Ghaffari-Moghaddam, H. Eslahi, Y.a. Aydin, and D. Saloglu, "Enzymatic processes in alternative reaction media: a mini review", J. Biol. Methods, vol. 2, pp. 25, 2015.
M. Solhtalab, H.R. Karbalaei-Heidari, and G. Absalan, "Tuning of hydrophilic ionic liquids concentration: A way to prevent enzyme instability", J. Mol. Catal., B Enzym., vol. 122, pp. 125-130, 2015..
S. Rezaei Motlagh, R. Harun, D.R. Awang Biak, S.A. Hussain, W.A. Wan Ab Karim Ghani, R. Khezri, C.D. Wilfred, A.A.M. Elgharbawy, and A.A.M. Elgharbawy, "Screening of Suitable ionic liquids as green solvents for extraction of Eicosapentaenoic Acid (EPA) from microalgae biomass using COSMO-RS Model", Molecules, vol. 24, no. 4, p. 713, 2019..
[ ] [PMID: 30781457]
P.L.G. Martins, A.R. Braga, and V.V. de Rosso, "Can ionic liquid solvents be applied in the food industry?", Trends Food Sci. Technol., vol. 66, pp. 117-124, 2017..
A.A.M. Elgharbawy, M. Moniruzzaman, and M. Goto, "Recent advances of enzymatic reactions in ionic liquids: Part II", Biochem. Eng. J., vol. 154, 2020..
A.A. Elgharbawy, F.A. Riyadi, M.Z. Alam, and M. Moniruzzaman, "Ionic liquids as a potential solvent for lipase-catalysed reactions: A review", J. Mol. Liq., vol. 251, pp. 150-166, 2018.
D.S.H. Wong, J.P. Chen, J.M. Chang, and C.H. Chou, "Phase equilibria of water and ionic liquids [emim][PF6] and", Fluid Phase Equilib., vol. 194–197, pp. 1089-1095, 2002..
T. Auxenfans, S. Buchoux, K. Djellab, C. Avondo, E. Husson, and C. Sarazin, "Mild pretreatment and enzymatic saccharification of cellulose with recycled ionic liquids towards one-batch process", Carbohydr. Polym., vol. 90, no. 2, pp. 805-813, 2012..
[ ] [PMID: 22840005]
K. Ninomiya, T. Yamauchi, C. Ogino, N. Shimizu, and K. Takahashi, "Microwave pretreatment of lignocellulosic material in cholinium ionic liquid for efficient enzymatic saccharification", Biochem. Eng. J., vol. 90, pp. 90-95, 2014..
A. Farghaly, M. Elsamadony, S. Ookawara, and A. Tawfik, "Bioethanol production from paperboard mill sludge using acidcatalyzed bio-derived choline acetate ionic liquid pretreatment followed by fermentation process", Energy Convers. Manage., vol. 145, pp. 255-264, 2017..
A.M. da Costa Lopes, K.G. João, D.F. Rubik, E. Bogel-Łukasik, L.C. Duarte, J. Andreaus, and R. Bogel-Łukasik, "Pre-treatment of lignocellulosic biomass using ionic liquids: Wheat straw fractionation", Bioresour. Technol., vol. 142, pp. 198-208, 2013..
[ ] [PMID: 23735803]
K. Ninomiya, K. Inoue, Y. Aomori, A. Ohnishi, C. Ogino, N. Shimizu, and K. Takahashi, "Characterization of fractionated biomass component and recovered ionic liquid during repeated process of cholinium ionic liquid-assisted pretreatment and fractionation", Chem. Eng. J., vol. 259, pp. 323-329, 2015.
M.G. Montalbán, G. Víllora, and P. Licence, "Ecotoxicity assessment of dicationic versus monocationic ionic liquids as a more environmentally friendly alternative", Ecotoxicol. Environ. Saf., vol. 150, pp. 129-135, 2018..
[ ] [PMID: 29272717]
A. Borkowski, Ł. Gutowski, M. Syczewski, T. Cłapa, and G. Czerwonka, "Adaptation of bacteria Escherichia coli in presence of quaternary ammonium ionic liquids", Ecotoxicol. Environ. Saf., vol. 164, pp. 370-378, 2018..
[ ] [PMID: 30138820]
I.R. Sitepu, S. Shi, B.A. Simmons, S.W. Singer, K. Boundy-Mills, and C.W. Simmons, "Yeast tolerance to the ionic liquid 1-ethyl-3- methylimidazolium acetate", FEMS Yeast Res., vol. 14, no. 8, pp. 1286-1294, 2014.
[ ] [PMID: 25348480]
N. Mehmood, E. Husson, C. Jacquard, S. Wewetzer, J. Büchs, C. Sarazin, and I. Gosselin, "Impact of two ionic liquids, 1-ethyl-3- methylimidazolium acetate and 1-ethyl-3-methylimidazolium methylphosphonate, on Saccharomyces cerevisiae: Metabolic, physiologic, and morphological investigations", Biotechnol. Biofuels, vol. 8, p. 17, 2015..
[ ] [PMID: 25688291]
Z-P. Zhao, X-L. Wang, G-Y. Zhou, Y. Cao, P. Lu, and W-F. Liu, "Hydrolysis kinetics of inulin by imidazole-based acidic ionic liquid in aqueous media and bioethanol fermentation", Chem. Eng. Sci., vol. 151, pp. 16-24, 2016.
N. Poornejad, K. Karimi, and T. Behzad, "Ionic liquid pretreatment of rice straw to enhance saccharification and bioethanol production", J. Biomass to Biofuel., vol. 1, pp. 8-15, 2014..
K. Ninomiya, C. Ogino, M. Ishizaki, M. Yasuda, N. Shimizu, and K. Takahashi, "Effect of post-pretreatment washing on saccharification and co-fermentation from bagasse pretreated with biocompatible cholinium ionic liquid", Biochem. Eng. J., vol. 103, pp. 198-204, 2015..
A.A. Elgharbawy, M.Z. Alam, M. Moniruzzaman, N.A. Kabbashi, and P. Jamal, "Chemical and structural changes of pretreated empty fruit bunch (EFB) in ionic liquid-cellulase compatible system for fermentability to bioethanol", 3 Biotech., vol. 8, Article number: 236, 2018..
G. Ramadoss, and K. Muthukumar, "Mechanistic study on ultrasound assisted pretreatment of sugarcane bagasse using metal salt with hydrogen peroxide for bioethanol production", Ultrason. Sonochem., vol. 28, pp. 207-217, 2016.
[ ] [PMID: 26384901]
A. Caputi, M. Ueda, and T. Brown, "Spectrophotometric determination of ethanol in wine", Am. J. Enol. Vitic., vol. 19, pp. 160-165, 1968..
M. Yu, J. Li, S. Chang, L. Zhang, Y. Mao, T. Cui, Z. Yan, C. Luo, and S. Li, "Bioethanol production using the sodium hydroxide pretreated sweet sorghum bagasse without washing", Fuel, vol. 175, pp. 20-25, 2016..
J.M. Dodić, D.G. Vučurović, S.N. Dodić, J.A. Grahovac, S.D. Popov, and N.M. Nedeljković, "Kinetic modelling of batch ethanol production from sugar beet raw juice", Appl. Energy, vol. 99, pp. 192-197, 2012..
K. Shill, S. Padmanabhan, Q. Xin, J.M. Prausnitz, D.S. Clark, and H.W. Blanch, "Ionic liquid pretreatment of cellulosic biomass: Enzymatic hydrolysis and ionic liquid recycle", Biotechnol. Bioeng., vol. 108, no. 3, pp. 511-520, 2011..
[ ] [PMID: 21246505]
M.O. Daramola, and L. Zampraka, "Experimental study of the production of biomass by Sacharomyces cerevisiae in a fed batch fermentor", Afr. J. Biotechnol., vol. 2008, pp. 7, 2008..
H. Shafaghat, G.D. Najafpour, P.S. Rezaei, and M. Sharifzadeh, "Growth kinetics and ethanol productivity of Saccharomyces cerevisiae PTCC 24860 on various carbon sources", World Appl. Sci. J., vol. 7, pp. 140-144, 2009..
P.M. Doran, Bioprocess engineering principles. Academic Press INC: San Diego, CA, 1995..
L.J. Jönsson, B. Alriksson, and N-O. Nilvebrant, "Bioconversion of lignocellulose: Inhibitors and detoxification", Biotechnol. Biofuels, vol. 6, no. 1, p. 16, 2013..
[ ] [PMID: 23356676]
H. Jin, R. Liu, and Y. He, "Kinetics of batch fermentations for ethanol production with immobilized saccharomyces cerevisiae growing on sweet sorghum stalk juice", Procedia Environ. Sci., vol. 12, pp. 137-145, 2012..
M. Papagianni, Y. Boonpooh, M. Mattey, and B. Kristiansen, "Substrate inhibition kinetics of Saccharomyces cerevisiae in fedbatch cultures operated at constant glucose and maltose concentration levels", J. Ind. Microbiol. Biotechnol., vol. 34, no. 4, pp. 301- 309, 2007..
[ ] [PMID: 17211636]
A. Osho, "Ethanol and sugar tolerance of wine yeasts isolated from fermenting cashew apple juice", Afr. J. Biotechnol., vol. 4, pp. 660- 662, 2005.
D. Stanley, A. Bandara, S. Fraser, P.J. Chambers, and G.A. Stanley, "The ethanol stress response and ethanol tolerance of Saccharomyces cerevisiae", J. Appl. Microbiol., vol. 109, no. 1, pp. 13-24, 2010..
[PMID: 20070446]
T. Srimachai, K. Nuithitikul, O. Sompong, P. Kongjan, and K. Panpong, "Optimization and kinetic modeling of ethanol production from oil palm frond juice in batch fermentation", Energy Procedia, vol. 79, pp. 111-118, 2015..
P. Ariyajaroenwong, P. Laopaiboon, A. Salakkam, P. Srinophakun, and L. Laopaiboon, "Kinetic models for batch and continuous ethanol fermentation from sweet sorghum juice by yeast immobilized on sweet sorghum stalks", J. Taiwan Inst. Chem. Eng., vol. 66, pp. 210-216, 2016.

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