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Nanoscience & Nanotechnology-Asia


ISSN (Print): 2210-6812
ISSN (Online): 2210-6820

Research Article

Sensitivity Enhancement in the Colorimetric/Spectroscopic Determination of Lysozyme Concentration in Nanomolar Level with Colloidal Citrat e Capped Au@Ag Core-shell Nanoparticles

Author(s): Debasish Aich, Rudra N. Mondal, Satyajit Saha, Pijus K. Samanta, Amit K. Bhunia and Tapanendu Kamilya*

Volume 11, Issue 4, 2021

Published on: 28 July, 2020

Article ID: e270421184207 Pages: 14

DOI: 10.2174/2210681210999200728114038

Price: $65


Background: Lysozyme level in body fluids is a significant indicator of various diseases. Cheap and simple colorimetric detection of lysozyme, in biological sample, by gold and silver nanoparticles is a field of interest of nanoparticle research for more than a decade.

Objective: We report here an attempt to improve the sensitivity part of the colorimetric lysozyme detection process by using citrate capped gold-silver core-shell nanoparticles without any functionalization.

Methods: Performance of gold-silver core-shell nanoparticles in determining of lysozyme concentration in water is done using colorimetric/spectroscopic technique. Gold nanoparticles, silver nanoparticles and mixed colloidal suspension of gold and silver nanoparticles, for comparison have also been studied. Selectivity study has been performed through spectroscopic analysis, red-greenblue colour component analysis and fractal dimension analysis of the nanoparticles interacted with several low and high isoelectric point proteins.

Results: Gold-silver core-shell nanoparticles showed higher sensitivity for a wider range of lysozyme concentration compared to gold and silver nanoparticles prepared by us or reported in literature. Gold nanoparticles showed higher sensitivity compared to the core-shell nanoparticles, but for a narrow concentration range of lysozyme. For silver nanoparticles and the mixed nanoparticle system, both sensitivity and range of determination of lysozyme concentration were much smaller compared to the core-shell nanoparticles. Core-shell nanoparticles showed better selectivity compared to gold nanoparticles in identifying aquatic solution of lysozyme from that of other proteins.

Conclusion: Gold-silver core-shell nanoparticles have higher sensitivity in determining wide range of lysozyme concentration in water compared to gold and silver nanoparticles reported in literature.

Keywords: Lysozyme, core-shell nanoparticles, spectroscopic, plasmon coupling, colorimetric, sensitivity.

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