Background: Amikacin is an aminoglycoside antibiotic used for many gram-negative bacterial infections like infections in the urinary tract, infections in brain, lungs and abdomen. Electrochemical determination of amikacin is a challenge in electroanalysis because it shows no voltammetric peak at the surface of bare electrodes.
Objective: In this approach, a very simple and easy method for indirect voltammetric determination of amikacin presented in real samples. Gold nanoparticles were electrodeposited at the surface of glassy carbon electrode in constant potential.
Methods: The effect of several parameters such as time and potential of deposition, pH and scan rates on signal were studied. The cathodic peak current of Au3+ decreased with increasing amikacin concentration. Quantitative analysis of amikacin was performed using differential pulse voltammetry by following cathodic peak current of gold ions.
Results: Two dynamic linear ranges of 1.0 × 10−8–1.0 × 10-7 M and 5.0 × 10−7–1.0 × 10-3 M were obtained and limit of detection was estimated 3.0× 10−9 M.
Conclusion: The method was successfully determined amikacin in pharmaceutical preparation and human serum. The effect of several interference in determination of amikacin was also studied.
[http://dx.doi.org/10.1016/j.talanta.2015.12.057 ] [PMID: 26838437]
[http://dx.doi.org/10.1016/j.saa.2019.02.067 ] [PMID: 30807943]
[http://dx.doi.org/10.1016/j.jpba.2005.09.008 ] [PMID: 16242884]
[http://dx.doi.org/10.1016/j.jchromb.2008.12.011 ] [PMID: 19117808]
[http://dx.doi.org/10.1039/C5NR08372A ] [PMID: 26732202]
[http://dx.doi.org/10.1088/0957-4484/27/37/374001 ] [PMID: 27505356]
[http://dx.doi.org/10.1016/j.bioelechem.2006.05.002 ] [PMID: 16793348]