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Current Medicinal Chemistry

Editor-in-Chief

ISSN (Print): 0929-8673
ISSN (Online): 1875-533X

Review Article

NMR Spectroscopy in the Conformational Analysis of Peptides: An Overview

Author(s): Marian Vincenzi, Flavia Anna Mercurio and Marilisa Leone*

Volume 28 , Issue 14 , 2021

Published on: 02 July, 2020

Page: [2729 - 2782] Pages: 54

DOI: 10.2174/0929867327666200702131032

Price: $65

Abstract

Background: NMR spectroscopy is one of the most powerful tools to study the structure and interaction properties of peptides and proteins from a dynamic perspective. Knowing the bioactive conformations of peptides is crucial in the drug discovery field to design more efficient analogue ligands and inhibitors of protein-protein interactions targeting therapeutically relevant systems.

Objective: This review provides a toolkit to investigate peptide conformational properties by NMR.

Methods: Articles cited herein, related to NMR studies of peptides and proteins were mainly searched through PubMed and the web. More recent and old books on NMR spectroscopy written by eminent scientists in the field were consulted as well.

Results: The review is mainly focused on NMR tools to gain the 3D structure of small unlabeled peptides. It is more application-oriented as it is beyond its goal to deliver a profound theoretical background. However, the basic principles of 2D homonuclear and heteronuclear experiments are briefly described. Protocols to obtain isotopically labeled peptides and principal triple resonance experiments needed to study them, are discussed as well.

Conclusion: NMR is a leading technique in the study of conformational preferences of small flexible peptides whose structure can be often only described by an ensemble of conformations. Although NMR studies of peptides can be easily and fast performed by canonical protocols established a few decades ago, more recently we have assisted to tremendous improvements of NMR spectroscopy to investigate instead large systems and overcome its molecular weight limit.

Keywords: NMR, structural biology, peptides, proteins, conformational ensemble, distance and angular restraints, bioactive conformation.


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