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Anti-Infective Agents

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ISSN (Print): 2211-3525
ISSN (Online): 2211-3533

Research Article

Evaluation of Anti-amoebic Activity of Spring Leaf Extract of Artemisia Aucheri on Acanthamoeba (T4 strain) In vitro

Author(s): Amir KarimiPourSaryazdi, Yeganeh KarimiPourSaryazdi, Fatemeh Ghaffarifar*, Maryam Ahmadi and Leila Zaki*

Volume 19 , Issue 2 , 2021

Published on: 29 June, 2020

Page: [140 - 146] Pages: 7

DOI: 10.2174/2211352518999200629165737

Price: $65

Abstract

Background: Acanthamoeba belongs to the free-living amoebae genus that causes a widespread infection with different severities in humans and animals. Treatment of these opportunistic protozoan infections is still not safe or effective. The aim of the present study was to evaluate the effect of spring extract of Artemisia aucheri on Acanthamoeba in vitro.

Methods: Trophozoites and cysts of Acanthamoeba were cultured on non-nutrient agar plates containing bacteria Escherichia coli. Macrophage cells were cultured in RPMI-1640 medium.

Different concentrations of spring extracts of A. aucheri were added to cultivated parasites and macrophages and additionally calculated to determine the 50% inhibitory concentration (IC50) for parasites and 50% cytotoxic concentration (CC50) for macrophages. Then, the viability of parasites were evaluated using counting assay. MTT assay was carried out on macrophage cells based on growth inhibition. Furthermore, in order to determine the possible induction of apoptosis in cysts of Acanthamoeba, flow cytometry method was employed using annexin staining.

Results: The effect of A. aucheri on trophozoites and cysts of Acanthamoeba was effective and had a reverse relationship with its concentration. The IC50 value of spring extract on trophozoites and cysts of the parasite was estimated to be 200 and 333 μg/ml at 24 h, respectively. According to the results of flow cytometry, the spring A. aucheri extract caused about 20% apoptosis.

Conclusion: Regarding the above results, it can be concluded that spring A. aucheri extract was effective against trophozoite and cyst forms of Acanthamoeba in vitro. Therefore, this plant could be an appropriate herbal drug candidate for the treatment of Acanthamoeba infections.

Keywords: Trophozoite, cyst, acanthamoeba, artemisia aucheri, in vitro, T4 strain.

Graphical Abstract
[1]
Khan, N.A. Acanthamoeba: biology and increasing importance in human health. FEMS Microbiol. Rev., 2006, 30(4), 564-595.
[http://dx.doi.org/10.1111/j.1574-6976.2006.00023.x] [PMID: 16774587]
[2]
Visvesvara, G.S.; Moura, H.; Schuster, F.L. Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea. FEMS Immunol. Med. Microbiol., 2007, 50(1), 1-26.
[http://dx.doi.org/10.1111/j.1574-695X.2007.00232.x] [PMID: 17428307]
[3]
Marciano-Cabral, F.; Cabral, G. Acanthamoeba spp. as agents of disease in humans. Clin. Microbiol. Rev., 2003, 16(2), 273-307.
[http://dx.doi.org/10.1128/CMR.16.2.273-307.2003] [PMID: 12692099]
[4]
Anwar, A.; Khan, N.A.; Siddiqui, R. Galactose as novel target against Acanthamoeba cysts. PLoS Negl. Trop. Dis., 2019, 13(7)e0007385
[http://dx.doi.org/10.1371/journal.pntd.0007385] [PMID: 31348789]
[5]
Song, S.M.; Kang, S.A.; Park, H.K.; Kim, D.H.; Park, S.Y.; Jang, S.B.; Yu, H.S. Acanthamoeba profilin elicits allergic airway inflammation in mice. PLoS Negl. Trop. Dis., 2018, 12(12)e0006979
[http://dx.doi.org/10.1371/journal.pntd.0006979] [PMID: 30557322]
[6]
Wu, D.; Qiao, K.; Feng, M.; Fu, Y.; Cai, J.; Deng, Y.; Tachibana, H.; Cheng, X. Apoptosis of Acanthamoeba castellanii trophozoites induced by oleic acid. J. Eukaryot. Microbiol., 2018, 65(2), 191-199.
[http://dx.doi.org/10.1111/jeu.12454] [PMID: 28787535]
[7]
Huang, J-M.; Chang, Y-T.; Lin, W-C. The Biochemical and Functional Characterization of M28 Aminopeptidase Protein Secreted by Acanthamoeba spp. on Host Cell Interaction. Molecules, 2019, 24(24), 4573.
[http://dx.doi.org/10.3390/molecules24244573] [PMID: 31847255]
[8]
Magistrado-Coxen, P.; Aqeel, Y.; Lopez, A.; Haserick, J.R.; Urbanowicz, B.R.; Costello, C.E. The most abundant cyst wall proteins of Acanthamoeba castellanii are cellulose-binding lectins from three gene families that localize to distinct structures in cyst walls. bioRxiv, 2018.496307
[9]
Siddiqui, R.; Khan, N.A. Biology and pathogenesis of Acanthamoeba. Parasit. Vectors, 2012, 5(1), 6.
[http://dx.doi.org/10.1186/1756-3305-5-6] [PMID: 22229971]
[10]
Randag, A.C.; van Rooij, J.; van Goor, A.T.; Verkerk, S.; Wisse, R.P.L.; Saelens, I.E.Y.; Stoutenbeek, R.; van Dooren, B.T.H.; Cheng, Y.Y.Y.; Eggink, C.A. The rising incidence of Acanthamoeba keratitis: A 7-year nationwide survey and clinical assessment of risk factors and functional outcomes. PLoS One, 2019, 14(9)e0222092
[http://dx.doi.org/10.1371/journal.pone.0222092] [PMID: 31491000]
[11]
Moon, E-K.; Choi, H-S.; Kong, H-H.; Quan, F-S. Polyhexamethylene biguanide and chloroquine induce programmed cell death in Acanthamoeba castellanii. Exp. Parasitol., 2018, 191, 31-35.
[http://dx.doi.org/10.1016/j.exppara.2018.06.002] [PMID: 29885293]
[12]
Kosrirukvongs, P.; Wanachiwanawin, D.; Visvesvara, G.S. Treatment of acanthamoeba keratitis with chlorhexidine. Ophthalmology, 1999, 106(4), 798-802.
[http://dx.doi.org/10.1016/S0161-6420(99)90169-0] [PMID: 10201605]
[13]
Seal, D.; Hay, J.; Kirkness, C.; Morrell, A.; Booth, A.; Tullo, A.; Ridgway, A.; Armstrong, M. Successful medical therapy of Acanthamoeba keratitis with topical chlorhexidine and propamidine. Eye (Lond.), 1996, 10(Pt 4), 413-421.
[http://dx.doi.org/10.1038/eye.1996.92] [PMID: 8944089]
[14]
Hadas, E.; Ozarowski, M.; Derda, M.; Thiem, B.; Cholewinski, M.; Skrzypczak, L.; Gryszczynska, A.; Piasecka, A. THE USE OF EXTRACTS FROM PASSIFLORA SPP. IN HELPING THE TREATMENT OF ACANTHAMOEBIASIS. Acta Pol. Pharm., 2017, 74(3), 921-928.
[PMID: 29513962]
[15]
Pazoki, R; Raeeni, MG; Ghaffarifar, F; Saryazdi, AK; Bineshian, F F The Effect of Aqueous Extract of Artemisia aucheri Seed on Acanthamoeba In vitro., Journal of Pharmaceutical Research International., 2019. 1-0..
[16]
KarimiPourSaryazdi. A.; Ghaffarifar, F.; Dalimi, A.; Dayer, M.S. In-vitro and in-vivo comparative effects of the spring and autumn-harvested Artemisia aucheri Bioss extracts on Leishmania major. J. Ethnopharmacol., 2020.257112910
[http://dx.doi.org/10.1016/j.jep.2020.112910] [PMID: 32344159]
[17]
Albakhit, S.; Khademvatan, S.; Doudi, M.; Foroutan-Rad, M. Antileishmanial activity of date (Phoenix dactylifera L) fruit and pit extracts in vitro. J. Evid. Based Complementary Altern. Med., 2016, 21(4), NP98-NP102.
[http://dx.doi.org/10.1177/2156587216651031] [PMID: 27242378]
[18]
Organization, W.H. A report of the consultation meeting on traditional and modern medicine: harmonizing two approaches, 22–26 November 1999; West Pacific Region: Beijing, China, 2000.
[19]
Arab-Mazar, Z.; Kheirandish, F.; Rajaeian, S. Anti-toxoplasmosis activity of herbal medicines: Narrative review. Herbal Medicines Journal., 2017, 2(1), 43-49.
[20]
Bahmani, M.; Saki, K.; Rafieian-Kopaei, M.; Karamati, S.A.; Eftekhari, Z.; Jelodari, M. The most common herbal medicines affecting Sarcomastigophora branches: a review study. Asian Pac. J. Trop. Med., 2014, 7S1, S14-S21.
[http://dx.doi.org/10.1016/S1995-7645(14)60198-X] [PMID: 25312109]
[21]
Dodangeh, S.; Niyyati, M.; Kamalinejad, M. Anti-Acanthamoeba activities of chloroformic fractions of Trigonella Foenum graecum (seed) and their cytotoxity on mice macrophage cell. Novelty in Biomedicine., 2015, 3(4), 182-188.
[22]
Degerli, S.; Berk, S.; Malatyali, E.; Tepe, B. Screening of the in vitro amoebicidal activities of Pastinaca armenea (Fisch. & C.A.Mey.) and Inula oculus-christi (L.) on Acanthamoeba castellanii cysts and trophozoites. Parasitol. Res., 2012, 110(2), 565-570.
[http://dx.doi.org/10.1007/s00436-011-2524-z] [PMID: 21735149]
[23]
Bouyer, S.; Imbert, C.; Daniault, G.; Cateau, E.; Rodier, M-H. Effect of caspofungin on trophozoites and cysts of three species of Acanthamoeba. J. Antimicrob. Chemother., 2007, 59(1), 122-124.
[http://dx.doi.org/10.1093/jac/dkl451] [PMID: 17079240]
[24]
Mojarrab, M; Shiravand, A; Delazar, A; Heshmati Afshar, F. Evaluation of in vitro antimalarial activity of different extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and fractions of the most potent extracts. The Scientific World Journal. 2014, 2014.
[25]
Hosseini, S.; Kappas, M.; Chahouki, M.Z.; Gerold, G.; Erasmi, S.; Emam, A.R. Modelling potential habitats for Artemisia sieberi and Artemisia aucheri in Poshtkouh area, central Iran using the maximum entropy model and geostatistics. Ecol. Inform., 2013, 18, 61-68.
[http://dx.doi.org/10.1016/j.ecoinf.2013.05.002]
[26]
Baghini, G.S.; Sepahi, A.A.; Tabatabaei, R.R.; Tahvildari, K. The combined effects of ethanolic extract of Artemisia aucheri and Artemisia oliveriana on biofilm genes expression of methicillin resistant Staphylococcus aureus. Iran. J. Microbiol., 2018, 10(6), 417-423.
[PMID: 30873270]
[27]
Ahmadi, F.; Mojarrab, M.; Ghazi-Khansari, M.; Hosseinzadeh, L. A semipolar fraction of petroleum ether extract of Artemisia aucheri induces apoptosis and enhances the apoptotic response to doxorubicin in human neuroblastoma SKNMC cell line. Res. Pharm. Sci., 2015, 10(4), 335-344.
[PMID: 26600860]
[28]
Ghaffarifar, F.; Esavand Heydari, F.; Dalimi, A.; Hassan, Z.M.; Delavari, M.; Mikaeiloo, H. Evaluation of apoptotic and antileishmanial activities of Artemisinin on promastigotes and BALB/C mice infected with Leishmania major. Iran. J. Parasitol., 2015, 10(2), 258-267.
[PMID: 26246824]
[29]
Asgary, S.; Dinani, N.J.; Madani, H.; Mahzouni, P. Ethanolic extract of Artemisia aucheri induces regression of aorta wall fatty streaks in hypercholesterolemic rabbits. Pharmazie, 2008, 63(5), 394-397.
[PMID: 18557427]
[30]
Nayeri Chegeni, T.; Ghaffarifar, F.; Pirestani, M.; Dalimi Asl, A.; Maspi, N. Genotyping of Acanthamoeba Species Isolated from Keratitis Patients by PCR Sequencing Methods in Tehran, Iran. Int. J. Med. Lab., 2019, 6(4), 259-265.
[http://dx.doi.org/10.18502/ijml.v6i4.2002]
[31]
Zaki, L.; Ghaffarifar, F.; Sharifi, Z.; Horton, J.; Sadraei, J. Toxoplasma gondii: Preventive and therapeutic effects of morphine and evaluation of treatment parameters of tachyzoites and infected macrophages in vitro and in a murine model. EXCLI J., 2020, 19, 514-527.
[PMID: 32398975]
[32]
Ebrahimisadr, P.; Ghaffarifar, F.; Mohammad Hassan, Z. In-vitro evaluation of antileishmanial activity and toxicity of artemether with focus on its apoptotic effect. Iranian journal of pharmaceutical research. Iran. J. Pharm. Res., 2013, 12(4), 903-909.
[PMID: 24523770]
[33]
Khodadadi, M.; Nateghpour, M.; Souri, E.; Farivar, L.; Motevalli Haghi, A.; Rahimi-Froushani, A.; Karbalaei, Z. Evaluation of effectiveness of ethanolic extract of Artemisia aucheri, individually and in combination with chloroquine, on chloroquine-sensitive strain of Plasmodium berghei in sourian mice. Iran. J. Public Health, 2013, 42(8), 883-888.
[PMID: 26056643]
[34]
Ghazi-Khansaria, M.; Mojarrab, M.; Ahmadi, F.; Hosseinzadeh, L. The antiproliferative effects of petroleum ether extract of Artemisia aucheri on human cancerous cell lines. J Rep Pharm Sci., 2013, 2(2), 61-66.
[35]
Jang, E; Kim, B-J; Lee, K-T; Inn, K-S; Lee, J-H A survey of therapeutic effects of Artemisia capillaris in liver diseases. EvidenceBased Complementary and Alternative Medicine. 2015, 2015.
[36]
Kawazoe, K.; Tsubouchi, Y.; Abdullah, N.; Takaishi, Y.; Shibata, H.; Higuti, T.; Hori, H.; Ogawa, M. Sesquiterpenoids from Artemisia gilvescens and an anti-MRSA compound. J. Nat. Prod., 2003, 66(4), 538-539.
[http://dx.doi.org/10.1021/np020593m] [PMID: 12713410]
[37]
Utegenova, G.A.; Pallister, K.B.; Kushnarenko, S.V.; Özek, G.; Özek, T.; Abidkulova, K.T.; Kirpotina, L.N.; Schepetkin, I.A.; Quinn, M.T.; Voyich, J.M. Chemical composition and antibacterial activity of essential oils from Ferula L. species against methicillin-resistant Staphylococcus aureus. Molecules, 2018, 23(7), 1679.
[http://dx.doi.org/10.3390/molecules23071679] [PMID: 29996498]

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