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Current Pharmaceutical Design

Editor-in-Chief

ISSN (Print): 1381-6128
ISSN (Online): 1873-4286

Review Article

Sericin: A Versatile Protein Biopolymer with Therapeutic Significance

Author(s): Rasika Suryawanshi, Jovita Kanoujia, Poonam Parashar and Shubhini. A. Saraf*

Volume 26 , Issue 42 , 2020

Page: [5414 - 5429] Pages: 16

DOI: 10.2174/1381612826666200612165253

Price: $65

Abstract

Sericin is a unique proteinaceous biopolymer obtained from cocoons of Bombyx Mori. It has become very popular since it is bestowed with numerous health benefits. Sericin is composed of 18 types of amino acids, out of which 8 amino acids play a significant role in human metabolic pathways. Sericin is easily amenable to make into novel dosage forms and also has been conferred with numerous therapeutic activities such as wound healing, antihypertensive, neuro-protective, antitumor, anti-diabetic, anti-wrinkle, anti-ageing and antioxidant amongst various others. This review summarizes the current status of sericin, as a therapeutic moiety with a focus on active constituents as well as their proposed mechanism in the treatment of various chronic diseases. It also summarizes previous and current in-vitro, in-vivo, cell lines studies and clinical trials based pieces of evidence corroborating the therapeutic activities of sericin.

Keywords: Sericin, silk nanoparticles, protein nanoparticles, Bombyx Mori, novel drug delivery, therapeutic.

[1]
Kanoujia J, Singh M, Singh P, et al. Genipin crosslinked soy-whey based bioactive material for atorvastatin loaded nanoparticles: preparation, characterization and in vivo antihyperlipidemic study. RSC Advances 2016; 6: 93275-87.
[http://dx.doi.org/10.1039/C6RA16830B]
[2]
Kanoujia J, Singh M, Singh P, Saraf SA. Novel genipin crosslinked atorvastatin loaded sericin nanoparticles for their enhanced antihyperlipidemic activity. Mater Sci Eng C 2016; 69: 967-76.
[http://dx.doi.org/10.1016/j.msec.2016.08.011] [PMID: 27612792]
[3]
Patel R, Modasiya M. Sericin: pharmaceutical applications. Int J Res Pharm Biomed Sci 2011; 2: 913-7.
[4]
Zhang X, Khan MMR, Yamamoto T, Tsukada M, Morikawa H. Fabrication of silk sericin nanofibers from a silk sericin-hope cocoon with electrospinning method. Int J Biol Macromol 2012; 50(2): 337-47.
[http://dx.doi.org/10.1016/j.ijbiomac.2011.12.006] [PMID: 22198656]
[5]
Chirila TV, Suzuki S, McKirdy NC. Further development of silk sericin as a biomaterial: comparative investigation of the procedures for its isolation from Bombyx mori silk cocoons. Prog Biomater 2016; 5: 135-45.
[http://dx.doi.org/10.1007/s40204-016-0052-8] [PMID: 27525204]
[6]
Pal RR, Parashar P, Singh I, Saraf SA. Tamanu oil potentiated novel sericin emulgel of levocetirizine: repurposing for topical delivery against DNCB-induced atopic dermatitis, QbD based development and in vivo evaluation. J Microencapsul 2019; 36(5): 432-46.
[http://dx.doi.org/10.1080/02652048.2019.1637474] [PMID: 31238752]
[7]
Aramwit P, Damrongsakkul S, Kanokpanont S, Srichana T. Properties and antityrosinase activity of sericin from various extraction methods. Biotechnol Appl Biochem 2010; 55(2): 91-8.
[http://dx.doi.org/10.1042/BA20090186] [PMID: 20055756]
[8]
Kapoor S, Kundu SC. Silk protein-based hydrogels: Promising advanced materials for biomedical applications. Acta Biomater 2016; 31: 17-32.
[http://dx.doi.org/10.1016/j.actbio.2015.11.034] [PMID: 26602821]
[9]
Khan M, Tsukada M. Electrospun silk sericin nanofibers for biomedical applications. In: Silk biomaterials for tissue engineering and regenerative medicine Elsevie. 2014; pp. 125-56.
[http://dx.doi.org/10.1533/9780857097064.1.125]
[10]
Joseph B, Raj SJ. Therapeutic applications and properties of silk proteins from Bombyx mori. Front Life Sci 2012; 6: 55-60.
[http://dx.doi.org/10.1080/21553769.2012.760491]
[11]
Gerstel MS, Place VA. Drug delivery device. In: Google Patents, 1976.
[12]
Parashar P, Tripathi CB, Arya M, et al. Biotinylated naringenin intensified anticancer effect of gefitinib in urethane-induced lung cancer in rats: favourable modulation of apoptotic regulators and serum metabolomics Artif Cells Nanomed Biotechnol 2018; 46(sup3): S598-610.
[http://dx.doi.org/10.1080/21691401.2018.1505738] [PMID: 30322263]
[13]
Aramwit P, Keongamaroon O, Siritientong T, Bang N, Supasyndh O. Sericin cream reduces pruritus in hemodialysis patients: a randomized, double-blind, placebo-controlled experimental study. BMC Nephrol 2012; 13: 119.
[http://dx.doi.org/10.1186/1471-2369-13-119] [PMID: 23006933]
[14]
Chen Z, Yang S, He Y, Song C, Liu Y. Effect of sericin on diabetic hippocampal growth hormone/insulin-like growth factor 1 axis. Neural Regen Res 2013; 8(19): 1756-64.
[PMID: 25206472]
[15]
Guo W, Deng L, Yu J, et al. Sericin nanomicelles with enhanced cellular uptake and pH-triggered release of doxorubicin reverse cancer drug resistance. Drug Deliv 2018; 25(1): 1103-16.
[http://dx.doi.org/10.1080/10717544.2018.1469686] [PMID: 29742945]
[16]
Kato N, Sato S, Yamanaka A, Yamada H, Fuwa N, Nomura M. Silk protein, sericin, inhibits lipid peroxidation and tyrosinase activity. Biosci Biotechnol Biochem 1998; 62(1): 145-7.
[http://dx.doi.org/10.1271/bbb.62.145] [PMID: 9501526]
[17]
Verma J, Kanoujia J, Parashar P, Tripathi CB, Saraf SA. Wound healing applications of sericin/chitosan-capped silver nanoparticles incorporated hydrogel. Drug Deliv Transl Res 2017; 7(1): 77-88.
[http://dx.doi.org/10.1007/s13346-016-0322-y] [PMID: 27565984]
[18]
Tyeb S, Kumar N, Kumar A, Verma V. Flexible agar-sericin hydrogel film dressing for chronic wounds. Carbohydr Polym 2018; 200: 572-82.
[http://dx.doi.org/10.1016/j.carbpol.2018.08.030] [PMID: 30177201]
[19]
Wang F, Wang Y, Tian C, et al. Fabrication of the FGF1-functionalized sericin hydrogels with cell proliferation activity for biomedical application using genetically engineered Bombyx mori (B. mori) silk. Acta Biomater 2018; 79: 239-52.
[http://dx.doi.org/10.1016/j.actbio.2018.08.031] [PMID: 30149211]
[20]
Wang F, Hou K, Chen W, et al. Transgenic PDGF-BB/sericin hydrogel supports for cell proliferation and osteogenic differentiation. Biomater Sci 2020; 8(2): 657-72.
[http://dx.doi.org/10.1039/C9BM01478K] [PMID: 31769455]
[21]
Qi C, Xu L, Deng Y, Wang G, Wang Z, Wang L. Sericin hydrogels promote skin wound healing with effective regeneration of hair follicles and sebaceous glands after complete loss of epidermis and dermis. Biomater Sci 2018; 6(11): 2859-70.
[http://dx.doi.org/10.1039/C8BM00934A] [PMID: 30259043]
[22]
Chen C-S, Zeng F, Xiao X, et al. Three-dimensionally printed silk-sericin-based hydrogel scaffold: A promising visualized dressing material for real-time monitoring of wounds. ACS Appl Mater Interfaces 2018; 10(40): 33879-90.
[http://dx.doi.org/10.1021/acsami.8b10072] [PMID: 30204403]
[23]
Wang Z, Zhang Y, Zhang J, et al. Exploring natural silk protein sericin for regenerative medicine: an injectable, photoluminescent, cell-adhesive 3D hydrogel. Sci Rep 2014; 4: 7064.
[http://dx.doi.org/10.1038/srep07064] [PMID: 25412301]
[24]
Napavichayanun S, Bonani W, Yang Y, Motta A, Aramwit P. Fibroin and polyvinyl alcohol hydrogel wound dressing containing silk sericin prepared using high-pressure carbon dioxide. Adv Wound Care (New Rochelle) 2019; 8(9): 452-62.
[http://dx.doi.org/10.1089/wound.2018.0856] [PMID: 31737425]
[25]
Kanoujia J, Parashar P, Singh M, Tripathi CB, Saraf SA. Genipin initiated crosslinked sericin/chitosan hydrogels: accelerated wound healing in an animal model. Der Pharmacia Lettre 2018; 10: 37-57.
[26]
Punyamoonwongsa P, Klayya S, Sajomsang W, Kunyanee C, Aueviriyavit S. Silk sericin semi-interpenetrating network hydrogels based on peg-diacrylate for wound healing treatment Int J Polymer Sci 2019; 2019.
[27]
Suktham K, Koobkokkruad T, Wutikhun T, Surassmo S. Efficiency of resveratrol-loaded sericin nanoparticles: Promising bionanocarriers for drug delivery. Int J Pharm 2018; 537(1-2): 48-56.
[http://dx.doi.org/10.1016/j.ijpharm.2017.12.015] [PMID: 29229512]
[28]
Das SK, Dey T, Kundu S. Fabrication of sericin nanoparticles for controlled gene delivery. RSC Advances 2014; 4: 2137-42.
[http://dx.doi.org/10.1039/C3RA44990D]
[29]
Cho KY, Moon JY, Lee YW, et al. Preparation of self-assembled silk sericin nanoparticles. Int J Biol Macromol 2003; 32(1-2): 36-42.
[http://dx.doi.org/10.1016/S0141-8130(03)00023-0] [PMID: 12719130]
[30]
de Freitas ED, Lima BM, Rosa PCP, da Silva MGC, Vieira MGA. Evaluation of proanthocyanidin-crosslinked sericin/alginate blend for ketoprofen extended release. Adv Powder Technol 2019; 30: 1531-43.
[http://dx.doi.org/10.1016/j.apt.2019.04.031]
[31]
Yalcin E, Kara G, Celik E, et al. Preparation and characterization of novel albumin-sericin nanoparticles as siRNA delivery vehicle for laryngeal cancer treatment. Prep Biochem Biotechnol 2019; 49(7): 659-70.
[http://dx.doi.org/10.1080/10826068.2019.1599395] [PMID: 31066619]
[32]
Huang L, Tao K, Liu J, et al. Design and fabrication of multifunctional sericin nanoparticles for tumor targeting and pH-responsive subcellular delivery of cancer chemotherapy drugs. ACS Appl Mater Interfaces 2016; 8(10): 6577-85.
[http://dx.doi.org/10.1021/acsami.5b11617] [PMID: 26855027]
[33]
Lv X, Wang H, Su A, Chu Y. A Novel approach for sericin-conjugated silver nanoparticle synthesis and their potential as microbicide candidates. J Microbiol Biotechnol 2018; 28(8): 1367-75.
[http://dx.doi.org/10.4014/jmb.1802.02054] [PMID: 30301314]
[34]
He H, Tao G, Wang Y, et al. In situ green synthesis and characterization of sericin-silver nanoparticle composite with effective antibacterial activity and good biocompatibility. Mater Sci Eng C 2017; 80: 509-16.
[http://dx.doi.org/10.1016/j.msec.2017.06.015] [PMID: 28866194]
[35]
Yue X, Lin H, Yan T, Zhang D, Lin H, Chen Y. Synthesis of silver nanoparticles with sericin and functional finishing to cotton fabrics. Fibers Polym 2014; 15: 716-22.
[http://dx.doi.org/10.1007/s12221-014-0716-8]
[36]
Shilpa M, Harisha K, Asha S, Parushuram N, Ranjana R, Sangappa Y. Silk sericin a bio-template for the synthesis of silver nanoparticles and characterization. In AIP Conference Proceedings AIP Publishing 2019; 150024.
[http://dx.doi.org/10.1063/1.5122573]
[37]
Akturk O, Gun Gok Z, Erdemli O, Yigitoglu M. One-pot facile synthesis of silk sericin-capped gold nanoparticles by UVC radiation: Investigation of stability, biocompatibility, and antibacterial activity. J Biomed Mater Res A 2019; 107(12): 2667-79.
[http://dx.doi.org/10.1002/jbm.a.36771] [PMID: 31393664]
[38]
Parushuram N, Asha S, Suma S, Krishna K, Neelakandan R, Sangappa Y. Green synthesis of high yield mono-dispersed gold nanoparticles using silk-sericin and characterization. In AIP Conference Proceedings AIP Publishing 2019; 150016.
[http://dx.doi.org/10.1063/1.5122565]
[39]
Oh H, Lee JY, Kim A, et al. Preparation of silk sericin beads using LiCl/DMSO solvent and their potential as a drug carrier for oral administration. Fibers Polym 2007; 8: 470-6.
[http://dx.doi.org/10.1007/BF02875867]
[40]
Khandai M, Chakraborty S, Sharma A, et al. Preparation and evaluation of algino-sericin mucoadhesive microspheres: An approach for sustained drug delivery. J Adv Pharm Educ Res 2010; 1: 48-60.
[41]
Gilotra S, Chouhan D, Bhardwaj N, Nandi SK, Mandal BB. Potential of silk sericin based nanofibrous mats for wound dressing applications. Mater Sci Eng C 2018; 90: 420-32.
[http://dx.doi.org/10.1016/j.msec.2018.04.077] [PMID: 29853108]
[42]
Bai M-Y, Wang D-T, Sin J. Sericin-based wound dressing with wound moisture indicator: In vitro and in vivo comparison study. Materialia 2018; 1: 37-45.
[http://dx.doi.org/10.1016/j.mtla.2018.07.003]
[43]
Aramwit P, Sangcakul A. The effects of sericin cream on wound healing in rats. Biosci Biotechnol Biochem 2007; 71(10): 2473-7.
[http://dx.doi.org/10.1271/bbb.70243] [PMID: 17928707]
[44]
Ersel M, Uyanikgil Y, Karbek Akarca F, et al. Effects of silk sericin on incision wound healing in a dorsal skin flap wound healing rat model. Med Sci Monit 2016; 22: 1064-78.
[http://dx.doi.org/10.12659/MSM.897981] [PMID: 27032876]
[45]
Lamboni L, Li Y, Liu J, Yang G. Silk sericin-functionalized bacterial cellulose as a potential wound-healing biomaterial. Biomacromolecules 2016; 17(9): 3076-84.
[http://dx.doi.org/10.1021/acs.biomac.6b00995] [PMID: 27467880]
[46]
Sano M, Tamada Y, Niwa K, Morita T, Yoshino G. Sulfated sericin is a novel anticoagulant influencing the blood coagulation cascade. J Biomater Sci Polym Ed 2009; 20(5-6): 773-83.
[http://dx.doi.org/10.1163/156856209X426853] [PMID: 19323889]
[47]
Seo C-W, Um IC, Rico CW, Kang MY. Antihyperlipidemic and body fat-lowering effects of silk proteins with different fibroin/sericin compositions in mice fed with high fat diet. J Agric Food Chem 2011; 59(8): 4192-7.
[http://dx.doi.org/10.1021/jf104812g] [PMID: 21384872]
[48]
Lapphanichayakool P, Sutheerawattananonda M, Limpeanchob N. Hypocholesterolemic effect of sericin-derived oligopeptides in high-cholesterol fed rats. J Nat Med 2017; 71(1): 208-15.
[http://dx.doi.org/10.1007/s11418-016-1050-9] [PMID: 27771849]
[49]
Ali MM, Arumugam SBA. Effect of crude extract of Bombyx mori coccoons in hyperlipidemia and atherosclerosis. J Ayurveda Integr Med 2011; 2(2): 72-8.
[http://dx.doi.org/10.4103/0975-9476.82527] [PMID: 21760692]
[50]
Onsa-ard A, Shimbhu D, Tocharus J, Sutheerawattananonda M, Pantan R, Tocharus C. Hypotensive and vasorelaxant effects of sericin-derived oligopeptides in rats ISRN pharmacology 2013; 2013.
[http://dx.doi.org/10.1155/2013/717529]
[51]
Li YG, Ji DF, Lin TB, Zhong S, Hu GY, Chen S. LI Y-g. Protective effect of sericin peptide against alcohol-induced gastric injury in mice. Chin Med J (Engl) 2008; 121(20): 2083-7.
[http://dx.doi.org/10.1097/00029330-200810020-00026] [PMID: 19080280]
[52]
Rattana S, Katisart T, Butiman C, Sungthong B. Antihyperglycemic effect of silkworm powder, fibroin and sericin from three Thai Silkworm (Bombyx mori Linn.) in Streptozotocin-induced diabetic rats. Pharmacogn J 2017; 9.
[http://dx.doi.org/10.5530/pj.2017.4.89]
[53]
Song C, Liu D, Yang S, Cheng L, Xing E, Chen Z. Sericin enhances the insulin-PI3K/AKT signaling pathway in the liver of a type 2 diabetes rat model. Exp Ther Med 2018; 16(4): 3345-52.
[http://dx.doi.org/10.3892/etm.2018.6615] [PMID: 30250521]
[54]
Zhaorigetu S, Yanaka N, Sasaki M, Watanabe H, Kato N. Inhibitory effects of silk protein, sericin on UVB-induced acute damage and tumor promotion by reducing oxidative stress in the skin of hairless mouse. J Photochem Photobiol B 2003; 71(1-3): 11-7.
[http://dx.doi.org/10.1016/S1011-1344(03)00092-7] [PMID: 14705634]
[55]
Kitisin T, Maneekan P, Luplertlop N. In-vitro characterization of silk sericin as an anti-aging agent. J Agric Sci 2013; 5: 54.
[56]
Padamwar MN, Pawar AP, Daithankar AV, Mahadik KR. Silk sericin as a moisturizer: an in vivo study. J Cosmet Dermatol 2005; 4(4): 250-7.
[http://dx.doi.org/10.1111/j.1473-2165.2005.00200.x] [PMID: 17168872]
[57]
Voegeli R. Sericin silk protein: unique structure and properties. Cosmetics Toiletries 1993; 108: 101-8.
[58]
Singh M. Cosmetotextiles: a new aspect of technical textiles Handbook of Cosmetic Science and Technology. 2014; 4.
[59]
Yamada H, Yamasaki K, Zozaki K. Nail cosmetics containing sericin Patent EP 2001 1632214: A1
[60]
Kim H, Lim YJ, Park J-H, Cho Y. Dietary silk protein, sericin, improves epidermal hydration with increased levels of filaggrins and free amino acids in NC/Nga mice. Br J Nutr 2012; 108(10): 1726-35.
[http://dx.doi.org/10.1017/S0007114511007306] [PMID: 22244094]
[61]
Nagai N, Murao T, Ito Y, Okamoto N, Sasaki M. Enhancing effects of sericin on corneal wound healing in rat debrided corneal epithelium. Biol Pharm Bull 2009; 32(5): 933-6.
[http://dx.doi.org/10.1248/bpb.32.933] [PMID: 19420767]
[62]
Nagai N, Murao T, Ito Y, Okamoto N, Sasaki M. Enhancing effects of sericin on corneal wound healing in Otsuka Long-Evans Tokushima fatty rats as a model of human type 2 diabetes. Biol Pharm Bull 2009; 32(9): 1594-9.
[http://dx.doi.org/10.1248/bpb.32.1594] [PMID: 19721238]
[63]
Sasaki M, Kato N, Watanabe H, Yamada H. Silk protein, sericin, suppresses colon carcinogenesis induced by 1,2-dimethylhydrazine in mice. Oncol Rep 2000; 7(5): 1049-52.
[http://dx.doi.org/10.3892/or.7.5.1049] [PMID: 10948337]
[64]
Kaewkorn W, Limpeanchob N, Tiyaboonchai W, Pongcharoen S, Sutheerawattananonda M. Effects of silk sericin on the proliferation and apoptosis of colon cancer cells. Biol Res 2012; 45(1): 45-50.
[http://dx.doi.org/10.4067/S0716-97602012000100006] [PMID: 22688983]
[65]
Zhaorigetu S, Sasaki M, Watanabe H, Kato N. Supplemental silk protein, sericin, suppresses colon tumorigenesis in 1,2-dimethylhydrazine-treated mice by reducing oxidative stress and cell proliferation. Biosci Biotechnol Biochem 2001; 65(10): 2181-6.
[http://dx.doi.org/10.1271/bbb.65.2181] [PMID: 11758907]
[66]
Dash R, Acharya C, Bindu PC, Kundu SC. Antioxidant potential of silk protein sericin against hydrogen peroxide-induced oxidative stress in skin fibroblasts. BMB Rep 2008; 41(3): 236-41.
[http://dx.doi.org/10.5483/BMBRep.2008.41.3.236] [PMID: 18377728]
[67]
Fan JB, Wu LP, Chen LS, Mao XY, Ren FZ. Antioxidant activities of silk sericin from silkworm Bombyx mori. J Food Biochem 2009; 33: 74-88.
[http://dx.doi.org/10.1111/j.1745-4514.2008.00204.x]
[68]
Devi R, Deori M, Devi D. Evaluation of antioxidant activities of silk protein Sericin secreted by silkworm Antheraea assamensis (Lepidoptera: Saturniidae). J Pharm Res 2011; 4: 4688-91.
[69]
Senakoon W, Nuchadomrong S, Sirimungkararat S, Senawong T, Kitikoon P. Antibacterial action of eri (samia ricini) sericin against Escherichia coli and Staphylococcus aureus. Asian J Food Agro-Ind 2009; 2: S222-8.
[70]
Baqai N, Wilding JP. Pathophysiology and aetiology of obesity. Medicine (Baltimore) 2015; 43: 73-6.
[http://dx.doi.org/10.1016/j.mpmed.2014.11.016]
[71]
Okazaki Y, Kakehi S, Xu Y, et al. Consumption of sericin reduces serum lipids, ameliorates glucose tolerance and elevates serum adiponectin in rats fed a high-fat diet. Biosci Biotechnol Biochem 2010; 74(8): 1534-8.
[http://dx.doi.org/10.1271/bbb.100065] [PMID: 20699593]

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