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Current Chromatography


ISSN (Print): 2213-2406
ISSN (Online): 2213-2414

Research Article

Chromatographic Assessment of Polyphenolic Profile and Total Phenolic Content and Antioxidant Activity of Common Leafy Vegetables in Bangladesh

Author(s): Mohammad Khairul Alam*, Rumana Tuli, Mohammad Sharif Khan, Abu Tareq Mohammad Abdullah, Maksuda Khatun, Mohammad Akhtaruzzaman and Sheikh Nazrul Islam

Volume 7, Issue 1, 2020

Page: [40 - 50] Pages: 11

DOI: 10.2174/2213240607999200421144940

Price: $65


Background: Polyphenolic compounds are known to provide health benefits and protect against degenerative chronic diseases. Utilization and identification of foods with a high content of these compounds are gaining greater attention nowadays.

Objective: The present study reports the total phenolic content (TPC), polyphenolic composition and antioxidant activity (DPPH, FRAP and TEAC) of 10 commonly consumed leafy vegetables growing in Bangladesh.

Materials and Methods: The samples were collected from different locations of Bangladesh and mixed together to ensure sample representativeness. Folin-Ciocalteu method was used for the analysis of TPC, and quantification of polyphenolic components was done by high-performance liquid chromatography (HPLC- DAD). Additionally, antioxidant activities of the selected vegetables were also analysed by utilizing DPPH, FRAP & TEAC.

Results and Discussion: TPC ranged from 23.64 ± 1.20 to 45.59 ± 3.04 mg gallic acid equivalent (GAE)/g freeze-dried sample (fds). The polyphenolic spectrum ranged from 0.30 ± 0.02 to 647.42 ± 147.12 mg/100 g fds; quantity and spectrum of which varied in the vegetables. Among the studied vegetables, Centella asiatica contained the highest amount of TPC (45.59 ± 3.04 mg GAE/g fds) and also exhibited high antioxidant capacities, as documented by DPPH, FRAP and TEAC assays. Moreover, Principal component analysis (PCA) of investigated variables clearly separated Centella asiatica from other samples.

Conclusion: Phenolic compounds being strong antioxidants reduce the risk of chronic diseases and the finding of this study would aware the people to take vegetables rich in phenolics. It would also fill up the data gap in the existing food composition table of Bangladesh.

Keywords: Antioxidants, HPLC-DAD, leafy vegetables, polyphenolic profile, principal component analysis, total phenolic content.

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