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Current Pharmaceutical Analysis


ISSN (Print): 1573-4129
ISSN (Online): 1875-676X

Research Article

Simultaneous Analysis of Sexual Stimulants and Anabolic Steroids as Adulterants in Dietary Supplements by High Performance Liquid Chromatography with Photodiode Array Detection

Author(s): Carine Viana*, Gabriela Zanella Marcon, Thais Ramos Dal Molin, Denise Bohrer, Paulo Cícero do Nascimento and Leandro M. de Carvalho

Volume 17, Issue 6, 2021

Published on: 15 April, 2020

Page: [767 - 773] Pages: 7

DOI: 10.2174/1573412916999200415175325


Background: The illegal virtual market for food supplements facilitates fraud and adulteration. Worldwide concern for consumer safety is growing on the part regulatory agencies, healthcare professionals and consumers.

Objective: This work aimed to evaluate the presence of sexual stimulants and anabolic steroids commonly used in the adulteration of dietary supplements through the development of a high performance liquid chromatography with the photodiode array detection (HPLC-DAD) method for the identification and quantification of these compounds.

Methods: The mobile phase composed of an ammonium acetate solution, acetonitrile and methanol led to the efficient separation of vardenafil, testosterone base, testosterone propionate, tadalafil, sildenafil and yohimbine.

Results: The assay was linear (r2> 0.999), precise (RSD% <0.5), accurate (99.1 to 105.2%), and the limits of detection and quantification were less than 0.05 and 0.15 μg/mL, respectively. Four samples of dietary supplements contained testosterone (n=1), tadalafil (n=2) and yohimbine (n=1) as adulterants. The adulterants found were in subtherapeutic doses, probably to reduce possible adverse effects and the action expected to appear natural. Since about 80% of adverse drug reactions are dose dependent, unpredictable adverse drug reactions are dose independent and based on idiosyncratic or allergic mechanisms or intolerance. Conclusion: The developed method is convenient and easily applicable for adulteration detection of the analyzed drugs in the multicomponent supplements.

Keywords: Adulterants, anabolic steroids, dietary supplements, liquid chromatography with photodiode array, sexual stimulants, dysfunction.

Graphical Abstract
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