Background: Ovarian cancer and ovarian related diseases affect reproductive health. Therapeutic molecules are needed to improve treatment outcomes and overcome drug resistance. The benzimidazole-based sulphonamide LLW-3-6 has both anti-apoptotic and anti-proliferative effects when used to treat prostate, breast, and brain cancer cells.
Objective: The study described herein evaluates the anti-proliferative and anti-migratory effects of LLW-3-6 in SKOV-3 ovarian cystadenocarcinoma cell line.
Methods: Studies were conducted using SKOV-3 cells treated with LLW-3-6. The cell line was propagated and proliferative activity was evaluated by hemocytometric and MTT colorimetric assays. Cellular apoptosis was assessed using caspase-3 spectrophotometric analysis. Lastly, a scratch wound assay was conducted at several concentrations and time points to assess the effect of LLW-3-6 on migration.
Results & Discussion: Proliferative studies suggest, SKOV-3 cells exposed to LLW-3-6 in culture resulted in decreased growth and proliferation of cells in a time and dose-dependent manner. The apoptotic effect of this agent was noted with the confirmed presence of Caspase-3 in a dose and time-dependent manner as well. Preliminary studies also suggest an anti-migratory effect of LLW-3- 6, confirmed by scratch wound analysis.
Conclusion: LLW-3-6 is potentially a chemotherapeutic option for decreasing proliferation and inducing apoptosis in ovarian carcinomas. Additional biological analysis are ongoing to further assess the utility of the molecule and its mechanism of action.
[http://dx.doi.org/10.3322/caac.21149] [PMID: 22700443]
[http://dx.doi.org/10.1001/jamaoncol.2014.161] [PMID: 26182310]
[http://dx.doi.org/10.2174/0929867322666150114164032] [PMID: 25620093]
[http://dx.doi.org/10.1016/j.bioorg.2015.09.003] [PMID: 26469740]
[http://dx.doi.org/10.3390/molecules17010873] [PMID: 22252503]