Login Register Cart 0
Generic placeholder image

The Natural Products Journal

Editor-in-Chief

ISSN (Print): 2210-3155
ISSN (Online): 2210-3163

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Anticancer Potential of Michelia champaca Linn. Bark Against Ehrlich Ascites Carcinoma (EAC) Cells in Swiss Albino Mice

Author(s): Ruksana Yesmin, Plabon Kumar Das, Hazrat Belal, Suraiya Aktar, Mst. Ayesha Siddika, Saharia Yeasmin Asha, Farjana Habib, Md. Abdur Rakib and Farhadul Islam*

Volume 11, Issue 1, 2021

Published on: 20 November, 2019

Page: [85 - 96] Pages: 12

DOI: 10.2174/2210315509666191120105647

Price: $65

Abstract

Background: Adverse side effects of currently available therapies against cancer, leads scientists to find effective compounds from natural sources.

Objective: In the present study, stem-bark of Mycelia champaca is subjected to evaluate its antiproliferative effect against Ehrlich Ascites Carcinoma (EAC) cells. To date, the anti-proliferative effects of M. champaca bark extract against EAC cell line has not been reported elsewhere. Therefore, we intended to investigate the anti-proliferative potential of M. champaca bark extract against EAC cells in vivo.

Methods: In vivo anticancer activity was evaluated against EAC cells bearing Swiss albino mice by monitoring parameters such as tumor cell proliferation, tumor weight measurement, and survival time, etc. The mechanism of EAC killing was examined by observation of cell morphology and analysis the expression of certain cancer-related genes. In vitro antioxidant potentiality was determined in terms of several common antioxidant assays. In addition, total phenolic and flavonoids contents were measured to ensure the presence of phytochemicals.

Results: M. champaca bark extract showed strong antioxidant activities which were found to be strongly correlated (P<0.001) with phenolics and flavonoids contents. Furthermore, it was found that bark extract decreased tumor cell proliferation (77.46%; P<0.01), tumor weight (42.13%; P<0.001) and increased life span of tumor-bearing mice (71.97%; P<0.01) at the dose of 250mg/kg (intraperitoneal; i.p.). M. champaca bark also altered the depleted hematological parameters such as red blood cell, white blood cell, hemoglobin (Hb%) towards normal in tumor bearing mice. In addition, upregulation of p53, Bax and downregulation of Bcl-2 followed by treatment indicated M. champaca bark could induce apoptosis of EAC cells.

Conclusion: These results indicated that MEMCB possesses significant cytotoxic activities against EAC cells and has a strong in vitro antioxidant capacity. Therefore, the bark of M. champaca could be considered as a potential resource of anti-cancer agents, which might be used to formulate effective anticancer drugs.

Keywords: Michelia champaca, chemotherapy, natural products, apoptosis, antioxidant, EAC cells.

Graphical Abstract

[1]
Tsai, C.J.; Riaz, N.; Gomez, S.L. Big data in cancer research: Real-World resources for precision oncology to improve cancer care delivery. Semin. Radiat. Oncol., 2019, 29(4), 306-310.
[http://dx.doi.org/10.1016/j.semradonc.2019.05.002] [PMID: 31472730]
[2]
Bosacki, C.; Vallard, A.; Gras, M.; Daguenet, E.; Morisson, S.; Méry, B.; Jmour, O.; Guy, J.B.; Magné, N. Complementary and alternative medicines in cancer patients. Bull. Cancer, 2019, 106(5), 479-491.
[http://dx.doi.org/10.1016/j.bulcan.2019.02.011] [PMID: 31023481]
[3]
Suja, K.P.; Jayalekshmy, A.; Arumughan, C. Free radical scavenging behavior of antioxidant compounds of sesame (Sesamum indicum L.) in DPPH(*) system. J. Agric. Food Chem., 2004, 52(4), 912-915.
[http://dx.doi.org/10.1021/jf0303621] [PMID: 14969550]
[4]
Rajkumar, V.; Guha, G.; Kumar, R.A. Antioxidant and anti-neoplastic activities of Picrorhiza kurroa extracts. Food Chem. Toxicol., 2011, 49(2), 363-369.
[http://dx.doi.org/10.1016/j.fct.2010.11.009] [PMID: 21081148]
[5]
Mann, C.D.; Neal, C.P.; Garcea, G.; Manson, M.M.; Dennison, A.R.; Berry, D.P. Phytochemicals as potential chemopreventive and chemotherapeutic agents in hepatocarcinogenesis. Eur. J. Cancer Prev., 2009, 18(1), 13-25.
[http://dx.doi.org/10.1097/CEJ.0b013e3282f0c090] [PMID: 19077560]
[6]
Doughari, J.H.; Human, I.S.; Bennade, S.; Ndakidemi, P.A. Phytochemicals as chemotherapeutic agents and antioxidants: Possible solution to the control of antibiotic resistant verocytotoxin producing bacteria. J. Med. Plants Res., 2009, 3, 839-848.
[7]
Ernst, E.; Cassileth, B.R. The prevalence of complementary/alternative medicine in cancer: A systematic review. Cancer, 1998, 83(4), 777-782.
[http://dx.doi.org/10.1002/(SICI)1097-0142(19980815)83:4<777:AIDCNCR22>3.0.CO;2-O] [PMID: 9708945]
[8]
Al-Mamun, M.A.; Husna, J.; Khatun, M.; Hasan, R.; Kamruzzaman, M.; Hoque, K.M.; Reza, M.A.; Ferdousi, Z. Assessment of antioxidant, anticancer and antimicrobial activity of two vegetable species of Amaranthus in Bangladesh. BMC Complement. Altern. Med., 2016, 16, 157.
[http://dx.doi.org/10.1186/s12906-016-1130-0] [PMID: 27246877]
[9]
Safarzadeh, E.; Sandoghchian Shotorbani, S.; Baradaran, B. Herbal medicine as inducers of apoptosis in cancer treatment. Adv. Pharm. Bull., 2014, 4(Suppl. 1), 421-427. [ 25364657
[10]
Rajesh, K.; Chitra, P.; Paarakh, M.; Chidambaranathan, N. Anticancer activity of aerial parts of Aervalanata Linn Juss ex Schult against Dalton’s Ascitic Lymphoma. Eur. J. Integr. Med., 2011, 3, 245-250.
[http://dx.doi.org/10.1016/j.eujim.2011.05.001]
[11]
Nayak, S.; Behera, S.K.; Mishra, M.K. Ethno-medico-botanical survey of Kalahandi district Orissa. Indian J. Tradit. Knowl., 2004, 3, 72-79.
[12]
Ahmed, H.; Mishra, A.; Gupta, R.; Saraf, S.A. Determination of quercetin in Michelia champaca L. (CHAMPA) leaves and stems bark by HPTLC. Int. J. Pharma Bio Sci., 2011, 2, 388-397.
[13]
Gupta, S.; Mehla, K.; Chauhan, D.; Nair, A. Anti-inflammatory activity of leaves of Michelia champaca investigated on acute inflammation induced rats. Lat. Am. J. Pharm., 2011, 30, 819-822.
[14]
Mullaicharam, A.R.; Kumar, M.S. Effect of Michelia champaca Linn onpylorous ligated rats. J. Appl. Pharm, 2011, 1, 60-64.
[15]
Jarald, E.E.; Joshi, S.B.; Jain, D.C. Antidiabetic activity of flower buds of Michelia champaca Linn. Indian J. Pharmacol., 2008, 40(6), 256-260.
[http://dx.doi.org/10.4103/0253-7613.45151] [PMID: 21279181]
[16]
Dama, G.; Bidkar, J.; Deore, S.; Jori, M.; Joshi, P. Helmintholytic activity of the methanolic and aqueous extracts of leaves of Michelia champaca. Res. J. Pharmacol. Pharmacody., 2011, 3, 25-26.
[17]
Vimala, R.; Nagarajan, S.; Alam, M.; Susan, T.; Joy, S. Antiinflammatory and antipyretic activity of Michelia champaca Linn., (white variety), Ixora brachiata Roxb. and Rhynchosia cana (Wild.) D.C. flower extract. Indian J. Exp. Biol., 1997, 35(12), 1310-1314.
[PMID: 9567766]
[18]
Khan, M.R.; Kihara, M.; Omoloso, A.D. Antimicrobial activity of Michelia champaca. Fitoterapia, 2002, 73(7-8), 744-748.
[http://dx.doi.org/10.1016/S0367-326X(02)00248-4] [PMID: 12490248]
[19]
Wolfe, K.; Wu, X.; Liu, R.H. Antioxidant activity of apple peels. J. Agric. Food Chem., 2003, 51(3), 609-614.
[http://dx.doi.org/10.1021/jf020782a] [PMID: 12537430]
[20]
Ordonez, A.A.L.; Gomez, J.D.; Vattuone, M.A.; Isla, M.I. Antioxidant activities of Sechiumedule (Jacq.) Swart extracts. Food Chem., 2006, 97, 452-458.
[http://dx.doi.org/10.1016/j.foodchem.2005.05.024]
[21]
Cheel, J.; Theoduloz, C.; Rodríguez, J.; Schmeda-Hirschmann, G. Free radical scavengers and antioxidants from Lemongrass (Cymbopogon citratus (DC.) Stapf.). J. Agric. Food Chem., 2005, 53(7), 2511-2517.
[http://dx.doi.org/10.1021/jf0479766] [PMID: 15796587]
[22]
Rani, P.; Unni, K.M.; Karthikeyan, J. Evaluation of antioxidant properties of berries. Indian J. Clin. Biochem., 2004, 19(2), 103-110.
[http://dx.doi.org/10.1007/BF02894266] [PMID: 23105465]
[23]
Klein, S.M.; Cohen, G. Cederbaum. Production of formaldehyde during metabolism of dimethyl sulphoxide by hydroxyl radical generating system. Biochemistry, 1981, 20, 6002-6012.
[http://dx.doi.org/10.1021/bi00524a013]
[24]
Khanam, J.A.; Islam, M.F.; Jesmin, M.; Ali, M.M. Antineoplastic activity of acetone semicarbazone (ASC) against Ehirlich ascites carcinoma (EAC) bearing mice. J. Natl. Sci. Found Sri., 2010, 38, 225-231.
[25]
Litchfield, J.T., Jr; Wilcoxon, F. A simplified method of evaluating dose-effect experiments. J. Pharmacol. Exp. Ther., 1949, 96(2), 99-113.
[PMID: 18152921]
[26]
Islam, F.; Ghosh, S.; Khanam, J.A. Antiproliferative and hepatoprotective activity of metabolites from Corynebacterium xerosis against Ehrlich Ascites Carcinoma cells. Asian Pac. J. Trop. Biomed., 2014, 4(Suppl. 1), S284-S292.
[http://dx.doi.org/10.12980/APJTB.4.2014C1283] [PMID: 25183099]
[27]
Rasida, P.; Islam, F.; Khanum, J.; Yeasmin, T. Preventive effect of ethanol extract of Alpiniacalcarata Rosc on Ehrlich’s ascitic carcinoma cell induced malignant ascites in mice. Asian Pac. J. Trop. Biomed., 2005, 5, 121-125.
[28]
Kabir, S.R.; Islam, M.F.; Alom, M.J.; Abu Zubair, M.; Absar, N. Purification, characterizations of a snake guard seeds lectin with antitumor activity against Ehrlich ascites carcinoma cells in vivo in mice. Protein Pept. Lett., 2012, 19(3), 360-368.
[http://dx.doi.org/10.2174/092986612799363154] [PMID: 22185504]
[29]
Rahman, S.N.S.; Wahab, N.W.; Malek, S.N.A. In Vitro morphological assessment of apoptosis induced by antiproliferative constituents from the rhizomes of Curcuma zedoaria. Evid. Based Complement. Alternat. Med., 2013, 23, 1-14.
[30]
Alam, A.K.; Hossain, A.S.; Khan, M.A.; Kabir, S.R.; Reza, M.A.; Rahman, M.M.; Islam, M.S.; Rahman, M.A.; Rashid, M.; Sadik, M.G. The antioxidative fraction of white mulberry induces apoptosis through regulation of p53 and NFκB in EAC cells. PLoS One, 2016, 11(12)e0167536
[http://dx.doi.org/10.1371/journal.pone.0167536] [PMID: 27936037]
[31]
Clarkson, D.; Burichenal, J.H. Preliminary screening of antineoplastic drugs. Prog. Clin. Cancer, 1965, 1, 625-629.
[PMID: 14283934]
[32]
Aravind, S.R.; Joseph, M.M.; Varghese, S.; Balaram, P.; Sreelekha, T.T. Antitumor and immunopotentiating activity of polysaccharide PST001 isolated from the seed kernel of Tamarindus indica: An in vivo study in mice. Sci. World J.,, 2012, 2012361382
[http://dx.doi.org/10.1100/2012/361382] [PMID: 22593679]
[33]
Brown, J.M.; Attardi, L.D. The role of apoptosis in cancer development and treatment response. Nat. Rev. Cancer, 2005, 5(3), 231-237.
[http://dx.doi.org/10.1038/nrc1560] [PMID: 15738985]
[34]
Quideau, S.; Deffieux, D.; Douat-Casassus, C.; Pouységu, L. Plant polyphenols: Chemical properties, biological activities, and synthesis. Angew. Chem. Int. Ed. Engl., 2011, 50(3), 586-621.
[http://dx.doi.org/10.1002/anie.201000044] [PMID: 21226137]
[35]
Zheng, W.; Wang, S.Y. Antioxidant activity and phenolic compounds in selected herbs. J. Agric. Food Chem., 2001, 49(11), 5165-5170.
[http://dx.doi.org/10.1021/jf010697n] [PMID: 11714298]
[36]
Fotsis, T.; Pepper, M.S.; Aktas, E.; Breit, S.; Rasku, S.; Adlercreutz, H.; Wähälä, K.; Montesano, R.; Schweigerer, L. Flavonoids, dietary-derived inhibitors of cell proliferation and in vitro angiogenesis. Cancer Res., 1997, 57(14), 2916-2921.
[PMID: 9230201]
[37]
Wagner, H.; Geyer, B.; Kiso, Y.; Hikino, H.; Rao, G.S. Coumestans as the main active principles of the liver drugs Eclipta alba and Wedelia calendulacea. Planta Med., 1986, 5(5), 370-374.
[http://dx.doi.org/10.1055/s-2007-969188] [PMID: 3797500]
[38]
Islam, M. ; Rahi, M.; Jahangir, C.A.; Rahman, M.H.; Jerin, I.; Amin, R.; Reza, M. In Vivo anticancer activity of basellaalba leaf and seed extracts against Ehrlich's Ascites Carcinoma (EAC) cell line. Evid. Based Complem. Alternat. Med., 2018, 2018.

Rights & Permissions Print Cite
Article Metrics
18
1
© 2024 Bentham Science Publishers | Privacy Policy