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Recent Patents on Biotechnology


ISSN (Print): 1872-2083
ISSN (Online): 2212-4012

Research Article

Detoxifying Action of Aqueous Extracts of Mucuna pruriens Seed and Mimosa pudica Root Against Venoms of Naja nigricollis and Bitis arietans

Author(s): Matthew P. Ameh, Mamman Mohammed, Yusuf P. Ofemile, Magaji G. Mohammed, Ada Gabriel and Akefe O. Isaac*

Volume 14, Issue 2, 2020

Page: [134 - 144] Pages: 11

DOI: 10.2174/1872208313666191025110019

Price: $65


Background: The World Health Organization included snakebite envenomation among Neglected Tropical Diseases in 2017. The importance of natural products from plants is enormous, given that most prescribed drugs originate from plants. Among this is Mucuna pruriens and Mimosa pudica, with many registered patents asserting their health benefits.

Objective: This study investigated the in vitro neutralizing effects of Mucuna pruriens seed and Mimosa pudica root extracts on venoms of Naja nigricollis and Bitis arietans.

Methods: In mice, the LD50 and phytochemical analysis of M. pruriens and M. pudica plant extracts were carried out prior to the evaluation of their haemolytic and fibrinolytic effect. Their effects on the activities of phospholipase A2 (PLA2) were also assessed.

Results: At a concentration of 50 mg/ml, both plant extracts were found to neutralize the fibrinolytic activity of N. nigricollis, but 400 mg/ml was required to neutralize the fibrinolytic activity of B. arietans. In haemolytic studies, 50 mg/ml concentration of M. pruriens extract suppressed haemolysis caused by N. nigricollis venom by 70% but at the same concentration, M. pudica extract reduced haemolysis by 49.4%. M. pruriens, at 50 mg/ml concentration, only inhibited phospholipase A2 activity by 7.7% but higher concentrations up to 400mg/ml had no effect against the venom of N. nigricollis; at 200 mg/ml. M. pudica extract inhibited PLA2 activity by 23%.

Conclusion: The results suggest that M. pruriens and M. pudica may be considered as promising antivenom agents for people living in a snake-bite prone environment.

Keywords: Snakes, in vitro, Mucuna pruriens, Mimosa pudica, venom, Naja nigricollis, Bitis arietans, antivenom.

Graphical Abstract
Chippaux JP. Snake-bites: appraisal of the global situation. Bull World Health Organ 1998; 76(5): 515-24.
[PMID: 9868843]
Ameh MP, Mamman M, Magaji MG, Yusuf PO, Ada G, Akefe IO. In vivo detoxifying action of the aqueous extract of Mucuna pruriens and Mimosa pudica against the venoms of Naja nigricollis and Bitis arietans. AJEMF 2019; 5: 20-6.
Ameh MP, Mamman M, Yusuf PO, Magaji MG, Ada G, Akefe IO, et al. Overview of the venomous snakes in West-Africa, clinical manifestation, diagnosis and treatment. J Med Biol 2019; 1: 11-26.
Alirol E, Sharma SK, Bawaskar HS, Kuch U, Chappuis F. Snake bite in South Asia: a review. PLoS Negl Trop Dis 2010; 4(1)e603
[] [PMID: 20126271]
World Health Organization. Neglected tropical diseases. Snakebite. 2015.
Salako LA. The control of snake bite in Nigeria Jos: National Council on Health Guest Lecture Series Nigeria 1994.
Farnsworth NR, Morris RW. Higher plants--the sleeping giant of drug development. Am J Pharm Sci Support Public Health 1976; 148(2): 46-52.
[PMID: 984178]
Rates SMK. Plants as source of drugs. Toxicon 2001; 39(5): 603-13.
[] [PMID: 11072038]
Mors WB, Nascimento MC, Pereira BMR, Pereira NA. Plant natural products active against snake bite--the molecular approach. Phytochemistry 2000; 55(6): 627-42.
[] [PMID: 11130675]
Maiorano VA, Marcussi S, Daher MA, et al. Antiophidian properties of the aqueous extract of Mikania glomerata. J Ethnopharmacol 2005; 102(3): 364-70.
[] [PMID: 16084045]
Soares AM, Ticli FK, Marcussi S, et al. Medicinal plants with inhibitory properties against snake venoms. Curr Med Chem 2005; 12(22): 2625-41.
[] [PMID: 16248818]
Ismaila MS, Adamu SA. The impact of traditional methods of managing snakebites in humans and livestock among the Hausa-Fulani communities of Sokoto State (North-Western Nigeria). J Plant Res 2012; 6: 4489-93.
Diogo LC, Fernandes RS, Marcussi S, et al. Inhibition of snake venoms and phospholipases A(2) by extracts from native and genetically modified Eclipta alba: isolation of active coumestans. Basic Clin Pharmacol Toxicol 2009; 104(4): 293-9.
[] [PMID: 19320636]
Yusuf PO, Mamman M, Ajagun E, Suleiman MM, Kawu MU, Shittu M, et al. Snakes responsible for bites in norths-eastern nigeria-a hospital based survey. J Environ Sci Toxicol Food Technol 2015; 9: 118-21.
Mallow D, Ludwig D, Nilson G. True vipers: natural history and toxinology of old world vipers. Florida: Krieger Publishing Company 2003; pp. 877-82.
Rainer PP, Kaufmann P, Smolle-Juettner FM, Krejs GJ. Case report: Hyperbaric oxygen in the treatment of puff adder (Bitis arietans) bite. Undersea Hyperb Med 2010; 37(6): 395-8.
[PMID: 21226389]
EchiTAb. Annual Medical records of antivenom treatment centre EchiTAb anti-snake study group UK/Nigeria. General Hospital Kaltungo, Gombe State, Nigeria; 2009.
Abubakar SB, Habib AG, Abubakar IS, Larnyang S, Durfa N, Nasidi A, et al. Factors affecting snakebite mortality in north-eastern Nigeria. J Int Health 2010; 3: 50-5.
World Health Organization. Guidelines for the Prevention and clinical management of snake bite in Africa. WHO Regional Office for Africa 2010.
Simpson ID, Jacobsen IM. Antisnake venom production crisis--who told us it was uneconomic and unsustainable? Wilderness Environ Med 2009; 20(2): 144-55.
[] [PMID: 19594208]
Biranchni NM, Mohanty CBK. Guidelines for Anti snake venom therapy: medicine update 2010; 20: 426-30.
Devi CM, Bai MV, Lal AV, Umashankar PR, Krishnan LK. An improved method for isolation of anti-viper venom antibodies from chicken egg yolk. J Biochem Biophys Methods 2002; 51(2): 129-38.
[] [PMID: 12062112]
Guerranti R, Aguiyi JC, Neri S, Leoncini R, Pagani R, Marinello E. Proteins from Mucuna pruriens and enzymes from Echis carinatus venom: characterization and cross-reactions. J Biol Chem 2002; 277(19): 17072-8.
[] [PMID: 11867642]
Meenatchisundaram S, Priyagrace S, Vijayaraghavan R, Velmurugan A, Parameswari G, Michael A. Antitoxin Activity of Mimosa pudica aqueous root extract against Naja naja and Bungarus caerulus venoms. Bangladesh J Pharmacol 2009; 4: 105-9.
Meenatchisundaram S, Michael A. Antitoxin Activity of Mucuna pruriens aqueous extract against Naja naja and Bungarus caerulus venoms. Int J Pharm Tech Res 2010; 2: 870-4.
Meenatchisundaram S, Michael A. Preliminary studies on antivenom activity of Mimosa pudica root extracts against russell’s viper and saw scaled viper venom by in vivo and in vitro methods. Pharmacologyonline 2009; 2: 372-4.
Public Health Service Policy on Humane Care and Use of Laboratory Animals. Washington, DC: US Department of Health and Human Services, USA 1996.
Macfarlane RG. Russell’s viper venom Br J Haematol 1967; 13(4): 437-51.
[] [PMID: 6067638]
Mirtschin PJ, Dunstan N, Hough B, et al. Venom yields from Australian and some other species of snakes. Ecotoxicology 2006; 15: 531-8.
Uhegbu FO, Elekwa I, Ukoha C. Comparative Efficacy of crude aqueous extract of papaya and sulphadoxine pyrimethamine in mice infested with malaria parasite in vivo. Glob J Pure Appl Sci 2005; 11: 399-401.
Theakston RDG, Reid HA. Development of simple standard assay procedures for the characterization of snake venom. Bull World Health Organ 1983; 61(6): 949-56.
[PMID: 6609011]
Bhat MK, Gowda TV. Purification and characterization of a myotoxic phospholipase A2 from Indian cobra (Naja naja naja) venom. Toxicon 1989; 27(8): 861-73.
[] [PMID: 2781585]
Gomes A, De P. Hannahpep: A novel fibrinolytic peptide from the Indian King Cobra (Ophiophagus hannah) venom. Biochem Biophys Res Commun 1999; 266(2): 488-91.
[] [PMID: 10600529]
Trease GE, Evans WC. Trease and Evans Pharmacognosy. 14th ed. London, W.: B Saunders Company Limite 1996; pp. 191-293.
Bruce RD. LD50 by up-and-down method. J Appl Toxicol 1985; 5: 151-7.
[] []
Fattepur SR, Gawade SP. Preliminary screening of herbal plant extracts for anti-venom activity against common sea snake (Enhydrina schistosa) poisoning. Pharmacogn Mag 2007; 3: 56-60.
Shobha SB. Antibacterial activity and phytochemical analysis of methanolic extract of Mucuna pruriens. Int J Pharm Pharmaceut Res 2017; 269-78.
Ibrahim ID, Mohammad I, Ashiru S, Sani I, Shehu K, Aliero AA, et al. Qualitative and quantitative phytochemical screening of Mimosa pudica plant extracts. Am J Biol Chem 2014; 2: 8-16.
Drug future Chemical Toxicity Database. Available at: http://www.
Hadimani GA, Desai SD, Biradar P, Nanjappaiah HM, Shivakumar H, Bagoji IB. Evaluation of acute oral toxicity and phytoconstituents of methanolic extract of Mucuna pruriens seed. J Pharm Sci Res 2015; 7: 33-6.
Karthikeyan M, Deepa MK. Antinociceptive Activity of Mimosa pudica Linn. Iranian J Pharmacol Therapeut 2010; 9: 11-4.
Wang Y, Ning ZH, Tai HW, et al. Relationship between lethal toxicity in oral administration and injection to mice: effect of exposure routes. Regul Toxicol Pharmacol 2015; 71(2): 205-12.
[] [PMID: 25558060]
Meenatchisundaram S, Sindhu M. In vivo and In vitro studies on neutralizing effects of Acorus calamus and Withania somnifera root extracts against Echis carinatus venom. Iran J Pharmacol Therapeut 2011; 10: 26-30.
James T, Dinesh MD, Uma MS, et al. In vivo and In vitro neutralizing potential of Rauvolfia serpentina plant extract against Daboia russelli venom. Adv Biol Res (Faisalabad) 2013; 7: 276-81.
Lee M. Pharmacodynamics of snake venoms and envenomation. In: Snakebites and Envenomation. Natural history of snakes, 2009.
Chu CW, Tsai TS, Tsai IH, Lin YS, Tu MC. Prey envenomation does not improve digestive performance in Taiwanese pit vipers (Trimeresurus gracilis and T. stejnegeri stejnegeri). Comp Biochem Physiol A Mol Integr Physiol 2009; 152(4): 579-85.
[] [PMID: 19256079]
Mahanta M, Mukherjee AK. Neutralisation of lethality, myotoxicity and toxic enzymes of Naja kaouthia venom by Mimosa pudica root extracts. J Ethnopharmacol 2001; 75(1): 55-60.
[] [PMID: 11282444]
Lees A, Olanow CW, Van Der Giessen R, Wagner H. Mucuna pruriens and extracts thereof for the treatment of neurological diseases WO2004039385A3 WIPO 2004.
Anderson JE, Declercq L. Compositions containing mimosa phenolic compounds. AU757715B2 2003.
Van Der Giessen R, Olanow CW, Lees A, Wagner H. Method for preparing Mucuna pruriens seed extract US7470441B2 2008.
Extraction method of total flavonoids of Mimosa pudica CN104922195A 2015.

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