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Anti-Infective Agents


ISSN (Print): 2211-3525
ISSN (Online): 2211-3533

Research Article

Essential Oil Composition and Anti-scabies Potential of Amomum subulatum Roxb. Leaves

Author(s): Bharat Sharma, Neeru Vasudeva* and Sunil Sharma

Volume 18, Issue 3, 2020

Page: [261 - 267] Pages: 7

DOI: 10.2174/2211352517666190919143631


Background: Scabies is an enervating parasitic infestation of skin caused by Sarcoptes scabiei, affecting besides 130 million people at any time. Globally, this neglected tropical disease is amenable for 0.07% of the total burden of disease. Amomum subulatum Rox. (Large Cardamom) plant parts are used in traditional medicine for curing dyspepsia, skin disease, anorexia, dysentery, hyperacidity, ulcers, wounds, cardiac debility, fever, cough, liver congestion and gonorrhoea.

Objective: The objective of this study was the phytochemical characterization of essential oil of A. subulatum leaves and evaluate its anti-scabies potential against S. scabiei.

Methods: Essential oil was collected by hydrodistillation of fresh leaves of A. subulatum using Clevenger apparatus and subjected to Gas Chromatography (GC), gas chromatography-mass spectrometry (GC-MS) for identification and quantification of components of oil. Anti-scabies potential of essential oil of leaves of A. subulatum against S. scabiei was investigated by contact bioassay method.

Results: GC and GC-MS analysis results revealed the presence of 39 constituents, of which terpinen-4- ol (29.87%), eucalyptol (18.69%), β-phallendrene (7.97%), γ-terpinene (6.67%), p-cymene (6.20%), were detected as major constituents. Oxygenated monoterpenes predominated in the A. subulatum essential oil, and constituted 59.03% of the total oil composition. The anti-scabies study demonstrated their scabicidal potential as its 10% concentration caused 100% mortality within 60 min.

Conclusion: The result indicated anti-scabies potential of essential oil of A. subulatum so can be used as an alternative for the treatment and effective control of S. scabiei.

Keywords: Large cardamom, essential oil, GC, GC-MS, anti-scabies activity, Sarcoptes scabiei.

Graphical Abstract
Bhat, S.A.; Mounsey, K.E.; Liu, X.; Walton, S.F. Host immune responses to the itch mite, Sarcoptes scabiei, in humans. Parasit. Vectors, 2017, 10(1), 385.
[] [PMID: 28797273]
Zhang, R.; Jise, Q.; Zheng, W.; Ren, Y.; Nong, X.; Wu, X.; Gu, X.; Wang, S.; Peng, X.; Lai, S.; Yang, G. Characterization and evaluation of a Sarcoptes scabiei allergen as a candidate vaccine. Parasit. Vectors, 2012, 5(1), 176.
[] [PMID: 22897929]
Bernigaud, C.; Fang, F.; Fischer, K.; Lespine, A.; Aho, L.S.; Dreau, D.; Kelly, A.; Sutra, J.F.; Moreau, F.; Lilin, T.; Botterel, F.; Guillot, J.; Chosidow, O. Preclinical study of single-dose moxidectin, a new oral treatment for scabies: efficacy, safety, and pharmacokinetics com-pared to two-dose ivermectin in a porcine model. PLoS Negl. Trop. Dis., 2016, 10(10) e0005030
[] [PMID: 27732588]
van der Linden, N.; van Gool, K.; Gardner, K.; Dickinson, H.; Agostino, J.; Regan, D.G.; Dowden, M.; Viney, R. A systematic review of scabies transmission models and data to evaluate the cost-effectiveness of scabies interventions. PLoS Negl. Trop. Dis., 2019, 13(3) e0007182
[] [PMID: 30849124]
Kumar, G.; Chauhan, B.; Ali, M. Anti-inflammatory effect of Amomum subulatum Roxb. fruits extract. Int. J. Pharm. Sci. Res., 2012, 3(12), 4722-4724.
Naik, J.P.; Rao, L.J.M.; Kumar, T.M.M.; Sampathu, S.R. Chemical composition of the volatile oil from the pericarp (husk) of large carda-mom (Amomum subulatum Roxb.). Flavour Fragrance J., 2004, 19(5), 441-444.
Bisht, V.K.; Negi, J.S.; Bhandari, A.K.; Sundriyal, R.C. Amomum subulatum Roxb: Traditional, phytochemical and biological activities- An overview. Afr. J. Agric. Res., 2011, 6(24), 5386-5390.
Joshi, R.; Sharma, P.; Sharma, V.; Prasad, R.; Sud, R.K.; Gulati, A. Analysis of the essential oil of large cardamom (Amomum subulatum Roxb.) growing in different agro-climatic zones of Himachal Pradesh, India. J. Sci. Food Agric., 2013, 93(6), 1303-1309.
[] [PMID: 23023817]
Tijjani, M.A.; Dimari, G.A.; Buba, S.W.; Khan, I.Z. In vitro antibacterial properties and pre-liminary phtytochemical analysis of Amomum subulatum Roxburg (Large Cardamom). J. Appl. Pharm. Sci., 2012, 2(5), 69-73.
Vavaiya, R.; Patel, A.; Manek, R. Anti-diabetic activity of Amomum subulatum Roxb. fruit constituents. Int. J. Pharm. Innov., 2010, 2, 50-65.
Bhandari, A.K.; Bisht, V.K.; Negi, J.S.; Baunthiyal, M. 1, 8-Cineole: A predominant component in the essential oil of large cardamom (Amomum subulatum Roxb.). J. Med. Plants Res., 2013, 7(26), 1957-1960.
Satyal, P.; Dosoky, N.S.; Kincer, B.L.; Setzer, W.N. Chemical compositions and biological activities of Amomum subulatum essential oils from Nepal. Nat. Prod. Commun., 2012, 7(9), 1233-1236.
[] [PMID: 23074918]
Shrestha, R.L. GC-MS analysis, antibacterial and antioxidant study of Amomum subulatum Roxb. J. Med. Plant Stud., 2017, 5(6), 126-129.
Naveed, R.; Hussain, I.; Tawab, A.; Tariq, M.; Rahman, M.; Hameed, S.; Mahmood, M.S.; Siddique, A.B.; Iqbal, M. Antimicrobial activity of the bioactive components of essential oils from Pakistani spices against Salmonella and other multi-drug resistant bacteria. BMC Complement. Altern. Med., 2013, 13(1), 265.
[] [PMID: 24119438]
Gilani, S.R.; Shahid, I.; Javed, M.; Mehmud, S.; Ahmed, R. Antimicrobial activities and physico-chemical properties of the essential oil from Amomum subulatum. Int. J. Appl. Chem., 2006, 2(2), 81-86.
Noumi, E.; Snoussi, M.; Alreshidi, M.M.; Rekha, P.D.; Saptami, K.; Caputo, L.; De Martino, L.; Souza, L.F.; Msaada, K.; Mancini, E.; Flamini, G.; Al-Sieni, A.; De Feo, V. Chemical and biological evaluation of essential oils from cardamom species. Molecules, 2018, 23(11), 2818.
[] [PMID: 30380739]
Owen, P.L.; Johns, T. Antioxidants in medicines and spices as cardioprotective agents in Tibetan highlanders. Pharm. Biol., 2002, 40(5), 346-357.
Verma, S.K.; Jain, V.; Singh, D.P. Effect of greater cardamom (Amomum subulatum Roxb.) on blood lipids, fibrinolysis and total antioxi-dant status in patients with ischemic heart disease. Asian Pac. J. Trop. Dis., 2012, 2, S739-S743.
Bairwa, G.L.; Jasuja, N.D.; Joshi, S.C. Lipid lowering and antioxidant effects of Amomum subulatum seeds (Family Zingiberaceae) in cholesterol fed rabbits. Arch. Phytopathol. Pflanzenschutz, 2011, 44(14), 1425-1431.
Singh, P.; Srivastava, B.; Kumar, A.; Dubey, N.K.; Gupta, R. Tanu, Efficacy of essential oil of Amomum subulatum as a novel aflatoxin B1 suppressor. J. Herbs Spices Med. Plants, 2008, 14, 208-218.
Jafri, M.A. Farah; Javed, K.; Singh, S. Evaluation of the gastric antiulcerogenic effect of large cardamom (fruits of Amomum subulatum Roxb). J. Ethnopharmacol., 2001, 75(2-3), 89-94.
[] [PMID: 11297839]
Govindarajan, M.; Rajeswary, M.; Senthilmurugan, S.; Vijayan, P.; Alharbi, N.S.; Kadaikunnan, S.; Khaled, J.M.; Benelli, G. Larvicidal activity of the essential oil from Amomum subulatum Roxb.(Zingiberaceae) against Anopheles subpictus, Aedes albopictus and Culex tri-taeniorhynchus (Diptera: Culicidae), and non-target impact on four mosquito natural enemies. Physiol. Mol. Plant Pathol., 2018, 101, 219-224.
Shukla, S.H.; Mistry, H.A.; Patel, V.G.; Jogi, B.V. Pharmacognostical, preliminary phytochemical studies and analgesic activity of Amomum subulatum Roxb. Pharma Sci. Monitor, 2010, 1(1), 90-102.
Essien, E.P.; Essien, J.P.; Ita, B.N.; Ebong, J.B. Phytochemical properties and fungitoxicity of the essential oil of Citrus medica L. against groundnut storage fungi. Turk. J. Bot., 2008, 32, 161-164.
Fang, F.; Candy, K.; Melloul, E.; Bernigaud, C.; Chai, L.; Darmon, C.; Durand, R.; Botterel, F.; Chosidow, O.; Izri, A.; Huang, W.; Guillot, J. In vitro activity of ten essential oils against Sarcoptes scabiei. Parasit. Vectors, 2016, 9(1), 594.
[] [PMID: 27876081]
Bhandari, A.K.; Baunthiyal, M.; Bisht, V.K.; Kaim, J.C.; Negi, B.S. Chemometric resolution for baseline identification of Amomum subu-latum Roxb. essential oils through FT-IR spectroscopy. Int. J. Org. Chem. (Irvine), 2017, 7(04), 319-324.
Kaskoos, R.A.; Mir, S.R.; Kapoor, R.; Ali, M. Essential oil composition of the fruits of Amomum subulatum Roxb. J. Essent. Oil-Bear Plants, 2013, 11(2), 184-187.
Jabbar, M.; Ghorbaniparvar, H. Determination of volatile components in black cardamom with gas chromatography-mass spectrometry and chemometric resolution. Int. J. Eng. Res. Tech., 2014, 11(3), 1280-1286.
Rout, P.K.; Sahoo, D.; Jena, K.S.; Rao, Y.R. Analysis of the oil of large cardamom (Amomum subulatum Roxb.,) growing in Sikkim. J. Essent. Oil Res., 2003, 15(4), 265-266.
Gurudutt, N.; Naik, J.P.; Srinivas, P. Volatile constituents of large cardamom (Amomum subulatum Roxb.). Flavour Fragrance J., 1996, 11, 7-9.
Hu, Z.; Chen, Z.; Yin, Z.; Jia, R.; Song, X.; Li, L.; Zou, Y.; Liang, X.; Li, L.; He, C.; Yin, L.; Lv, C.; Zhao, L.; Su, G.; Ye, G.; Shi, F. In vitro acaricidal activity of 1,8-cineole against Sarcoptes scabiei var. cuniculi and regulating effects on enzyme activity. Parasitol. Res., 2015, 114(8), 2959-2967.
[] [PMID: 25924796]
Pasay, C.; Mounsey, K.; Stevenson, G.; Davis, R.; Arlian, L.; Morgan, M.; Vyszenski-Moher, D.; Andrews, K.; McCarthy, J. Acaricidal activity of eugenol based compounds against scabies mites. PLoS One, 2010, 5(8) e12079
[] [PMID: 20711455]
Aboelhadid, S.M.; Mahrous, L.N.; Hashem, S.A.; Abdel-Kafy, E.M.; Miller, R.J. In vitro and in vivo effect of Citrus limon essential oil against sarcoptic mange in rabbits. Parasitol. Res., 2016, 115(8), 3013-3020.
[] [PMID: 27098160]
Zhou, Y.; Liao, F.; Weng, J.; Mo, Q.; Xu, R.; Zhang, Y.; Ren, Z.; Zhong, Z.; Zuo, Z.; Peng, G.; Deng, J.; Tang, C.; Hu, Y. Composition and acaricidal activity of essential oil from Elsholtzia densa Benth against Sarcoptes scabiei mites in vitro. Vet. Med., 2019, 64, 178-183.

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