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当代肿瘤药物靶点

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ISSN (Print): 1568-0096
ISSN (Online): 1873-5576

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Research Article

ERGIC3沉默可增加BFA对肺腺癌细胞的生长抑制作用

卷 20, 期 1, 2020

页: [67 - 75] 页: 9

弟呕挨: 10.2174/1568009619666190917145906

价格: $65

摘要

背景：已知布雷菲德菌素A（BFA）在癌细胞中诱导内质网应激（ERS）和高尔基体应激。 ERGIC3（内质网-高尔基体中间隔室3）是位于内质网和高尔基体中的II型跨膜蛋白。在癌细胞中经常观察到ERGIC3过表达。目的：在这项研究中，我们旨在探讨BFA与ERGIC3沉默同时给药是否会相加或协同抑制癌细胞的生长。 方法：用ERGIC3-siRNA敲低ERGIC3的表达，用BFA诱导肺癌细胞GLC-82和A549中的ERS。使用Q-RT-PCR和Western Blot分析检测ERGIC3和下游分子的表达。 GraphPad Prism 6用于量化数据。 结果：我们证明了通过siRNA沉默ERGIC3可以有效降低GLC-82和A549细胞中mRNA和蛋白质水平上ERGIC3的表达。虽然单独使用BFA或ERGIC3-沉默可以诱导ERS并抑制细胞生长，但是将ERGIC3-silencing和BFA联合用于肺癌细胞治疗可以通过上调GRP78来加重增强细胞生长的抑制作用，从而导致细胞周期停滞。 结论：ERGIC3沉默联合BFA治疗可加成抑制肺癌细胞的生长。这一发现可能为肺腺癌的新辅助疗法提供了启示。

关键词: ERGIC3，BFA，肺癌，细胞生长，内质网应激，腺癌细胞。

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