Anti-Bacterial and Crystallographic Studies of Jatrophone, the Macrocyclic Diterpenoid from the Roots of Jatropha Gossypifolia l.
Pp. 86-98 (13)
R. S. Satyan, Ajay Parida and Babu Varghese
Jatropha gossypifolia L., popularly known as the belly-ache bush is a rich natural source of novel
macrocyclic diterpenoids. Crude root and stem extracts of J. gossypifolia were tested against certain human
bacterial pathogens. Among the treatments, lipophilic hexane extract of the dried roots inhibited the gram
negative bacterial human pathogens Corynebacterium diphtheriae, Bacillus cereus and Proteus mirabilis.
Effective inhibition (18 mm) was observed against C. diphtheriae, the activity being dose-dependent among
the dilutions tested, with maximum efficacy at 500 ppm. B. cereus was resistant at lower dilutions (25-100
ppm), however, 250 and 500 ppm concentrations exhibited effective inhibition (8.5 and 9.1 mm). Marginal
inhibition was observed against P. mirabilis (5.5 mm) at 500 ppm. Of the 7 column fractions tested,
fraction-7 exhibited significant activity against C. diphtheriae, B. cereus, P. mirabilis, Shigella flexneri,
Klebsiella aerogenes and Staphylococcus aureus. Gram-negative organisms tested, except P. mirabilis and
S. flexneri, showed marginal inhibition for the crude extracts. Crystalline fraction-7 appreciably inhibited K.
aerogenes and S. aureus at higher concentrations (1000, 500 and 250 ppm). X-Ray Diffraction
Crystallography (XRD) analysis of the crystals confirmed that it is the known macrocyclic diterpenoid,
Jatrophone, with a few other proven biological activities.
Jatropha gossypifolia, Corynebacterium diphtheriae, Bacillus cereus, Proteus mirabilis, Jatrophone.
Bioprospecting & Tissue Culture Lab, M. S. Swaminathan Research Foundation, III Cross Street, Taramani Institutional Area, Taramani, Chennai- 600 113, India