Abstract
The objective of the study was to examine skeletal muscle regeneration capacity of young and very old rats during autotransplantation. In 3.5 and 30 month-old Wistar rats, gastrocnemius muscle was removed and grafted back to its original bed. Incorporation of 3H leucine into myofibrillar and sarcoplasmic protein fractions, their relative contents in autografts and synthesis rate of MyHC and actin were recorded. The relative muscle mass of old rats was about 67% of that of young rats; the absolute mass of autografted muscle was 61% intact in the young rat group and 51% in the old rat group. Content of myofibrillar protein in the autografts of young rats was 46% of the intact muscle content, and 39% in the old rat group.
In conclusion, the difference in skeletal muscle regeneration capacity of young and very old rats is about ten percent. In the autografts of both young and old rats, the regeneration of the contractile apparatus is less effective in comparison with the sarcoplasmic compartment.
Keywords: Aging, contractile apparatus, regeneration, skeletal muscle, regeneration, myofibrillar protein, sarcoplasmic compartment, autografted muscle
Current Aging Science
Title:Aging and Regenerative Capacity of Skeletal Muscle in Rats
Volume: 5 Issue: 2
Author(s): Priit Kaasik, Maire Aru, Karin Alev and Teet Seene
Affiliation:
Keywords: Aging, contractile apparatus, regeneration, skeletal muscle, regeneration, myofibrillar protein, sarcoplasmic compartment, autografted muscle
Abstract: The objective of the study was to examine skeletal muscle regeneration capacity of young and very old rats during autotransplantation. In 3.5 and 30 month-old Wistar rats, gastrocnemius muscle was removed and grafted back to its original bed. Incorporation of 3H leucine into myofibrillar and sarcoplasmic protein fractions, their relative contents in autografts and synthesis rate of MyHC and actin were recorded. The relative muscle mass of old rats was about 67% of that of young rats; the absolute mass of autografted muscle was 61% intact in the young rat group and 51% in the old rat group. Content of myofibrillar protein in the autografts of young rats was 46% of the intact muscle content, and 39% in the old rat group.
In conclusion, the difference in skeletal muscle regeneration capacity of young and very old rats is about ten percent. In the autografts of both young and old rats, the regeneration of the contractile apparatus is less effective in comparison with the sarcoplasmic compartment.
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Cite this article as:
Kaasik Priit, Aru Maire, Alev Karin and Seene Teet, Aging and Regenerative Capacity of Skeletal Muscle in Rats, Current Aging Science 2012; 5 (2) . https://dx.doi.org/10.2174/1874609811205020126
DOI https://dx.doi.org/10.2174/1874609811205020126 |
Print ISSN 1874-6098 |
Publisher Name Bentham Science Publisher |
Online ISSN 1874-6128 |
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