Abstract
An HPLC-UV method was developed for simultaneously quantifying 13 major components in Semen Oroxyli, a commonly used traditional Chinese medicinal herb, including scutellarin, quercetin-3-O-α-L-arabinopyranoside, oroxin B, chrysin-7-O-β-D-gentiobioside, baicalin, oroxin A, scutellarein, chrysin-7-O-β-D-glucuronide, quercetin, norwogonine, baicalein, chrysin and oroxylin A. The optimal conditions of separation and detection were achieved on an Agilent Zorbax SB-C18 column (250 mm x 4.6 mm, 5 μm) with a gradient elution of acetonitrile and 0.3% (v/v) acetic acid at a flow rate of 1.0 ml/min, with a detection wavelength of 277 nm. A complete separation was obtained within 50 min for the 13 target compounds. All calibration curves showed good linearity (r2 > 0.9997) within the testing range. The assay was reproducible with overall intra-and inter-day variation of less than 3%. The mean spiked recovery of all analytes was 100 ± 10%, with RSD less than 5%. The validated method was successfully applied to quantitatively analyse 13 flavonoids for quality evaluation of commercial Semen Oroxyli samples from different locations.
Keywords: Baicalin, Chrysin-7-O-β-D-glucuronide, Flavonoids, HPLC, Oroxin A, Oroxin B, Oroxylum indicum, Quantification, Scutellarein, Semen Oroxyli
Current Pharmaceutical Analysis
Title:Quality Evaluation of Semen Oroxyli through Simultaneous Quantification of 13 Components by High Performance Liquid Chromatography
Volume: 8 Issue: 2
Author(s): Yan-Qing Zhou, Qing-Wen Zhang, Song-Lin Li, Zhi-Qi Yin, Xiao-Qi Zhang and Wen-Cai Ye
Affiliation:
Keywords: Baicalin, Chrysin-7-O-β-D-glucuronide, Flavonoids, HPLC, Oroxin A, Oroxin B, Oroxylum indicum, Quantification, Scutellarein, Semen Oroxyli
Abstract: An HPLC-UV method was developed for simultaneously quantifying 13 major components in Semen Oroxyli, a commonly used traditional Chinese medicinal herb, including scutellarin, quercetin-3-O-α-L-arabinopyranoside, oroxin B, chrysin-7-O-β-D-gentiobioside, baicalin, oroxin A, scutellarein, chrysin-7-O-β-D-glucuronide, quercetin, norwogonine, baicalein, chrysin and oroxylin A. The optimal conditions of separation and detection were achieved on an Agilent Zorbax SB-C18 column (250 mm x 4.6 mm, 5 μm) with a gradient elution of acetonitrile and 0.3% (v/v) acetic acid at a flow rate of 1.0 ml/min, with a detection wavelength of 277 nm. A complete separation was obtained within 50 min for the 13 target compounds. All calibration curves showed good linearity (r2 > 0.9997) within the testing range. The assay was reproducible with overall intra-and inter-day variation of less than 3%. The mean spiked recovery of all analytes was 100 ± 10%, with RSD less than 5%. The validated method was successfully applied to quantitatively analyse 13 flavonoids for quality evaluation of commercial Semen Oroxyli samples from different locations.
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Cite this article as:
Zhou Yan-Qing, Zhang Qing-Wen, Li Song-Lin, Yin Zhi-Qi, Zhang Xiao-Qi and Ye Wen-Cai, Quality Evaluation of Semen Oroxyli through Simultaneous Quantification of 13 Components by High Performance Liquid Chromatography, Current Pharmaceutical Analysis 2012; 8 (2) . https://dx.doi.org/10.2174/1573412911208020206
DOI https://dx.doi.org/10.2174/1573412911208020206 |
Print ISSN 1573-4129 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-676X |
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