Novel Short AMP: Design and Activity Study

Author(s): Yoonkyung Park, Kyung-Soo Hahm

Journal Name: Protein & Peptide Letters

Volume 19 , Issue 6 , 2012

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Abstract:

In a previous study, we reported that truncation of HP (2-20) (derived from the N-terminal region of Helicobacter pylori Ribosomal Protein L1 (RPL1)) at the N- (residues 2-3) and C-terminal (residues 17-20) truncated fragments to give HP (4-16) induces increased antibiotic activity against several bacterial strains without hemolysis. In this study, to develop novel short antibiotic peptides useful as therapeutic drugs, an analogue was designed to possess increased hydrophobicity by Trp substitution in position 2 region of HP (4-16). Synthetic HP (4-16)-W showed an enhanced antimicrobial and antitumor activity. The antimicrobial activity of this peptide and others was measured by their growth inhibitory effect upon S. aureus, B. subtilis, S. epidermidis, E. coli, S. typimurium, P. aeruginosa, C. albicans, T. beigelii and S. cerevisiae. None of the peptides exhibited hemolytic activity against human erythrocyte cells except melittin as a positive control. Its antibiotic activity suggests that HP (4-16)-W is an excellent candidate as a lead compound for the development of novel antibiotic agents.

Keywords: Helicobacter pylori ribosomal protein L1, antimicrobial activity, HP (4-16)-W, hemolytic activity, antibiotic agents, erythrocyte cells, innate immunity, cecropin-like peptide, gastrointestinal illness, peptic ulcers

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Article Details

VOLUME: 19
ISSUE: 6
Year: 2012
Page: [652 - 656]
Pages: 5
DOI: 10.2174/092986612800494093
Price: $65

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