Title:Flow Cytometry Analysis and Crossmatch Detection Techniques in Transplantation
VOLUME: 12 ISSUE: 1
Author(s): Vincenzo Grimaldi, Elena Cesario, Amelia Casamassimi, Teresa Infante and Claudio Napoli
Affiliation:U.O.C. Immunohematology, Transfusion Medicine and Transplant Immunology [SIMT], Regional Reference Laboratory of Transplant Immunology [LIT], Azienda Universitaria Policlinico (AOU), 1st School of Medicine, Second University of Naples, Via Costantinopoli, 16, 80138, Naples (Italy).
Keywords:Anti-HLA antibodies, beads-based assay, cell-based assay, complement-dependent cytotoxicity crossmatch, flow cytometry crossmatch, luminex crossmatch, Luminex Single Antigen technology, monoclonal antibodies, organ transplantation, solid-phase assay, virtual crossmatch, XM-ONE
Abstract:The relevance of anti-human leucocyte antigen (anti-HLA) antibodies in the outcome of organ transplantation has been recognized in the past 40 years. Detection of these antibodies in sensitized recipients plays a pivotal role in this issue. Flow cytometry analysis is one of the choice cell-based techniques together with the pioneer complementdependent cytotoxicity crossmatch. Here, we discuss the advantages and limits of these cell-based assays and their use in the clinical laboratory of immunology of transplantation. Moreover, we compared this approach to other beads-based detection assays introduced more recently in the laboratory practice.
