Flow Cytometry Analysis and Crossmatch Detection Techniques in Transplantation

Author(s): Vincenzo Grimaldi, Elena Cesario, Amelia Casamassimi, Teresa Infante, Claudio Napoli

Journal Name: Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry (Under Re-organization)
Formerly Current Medicinal Chemistry - Immunology, Endocrine & Metabolic Agents

Volume 12 , Issue 1 , 2012


The relevance of anti-human leucocyte antigen (anti-HLA) antibodies in the outcome of organ transplantation has been recognized in the past 40 years. Detection of these antibodies in sensitized recipients plays a pivotal role in this issue. Flow cytometry analysis is one of the choice cell-based techniques together with the pioneer complementdependent cytotoxicity crossmatch. Here, we discuss the advantages and limits of these cell-based assays and their use in the clinical laboratory of immunology of transplantation. Moreover, we compared this approach to other beads-based detection assays introduced more recently in the laboratory practice.

Keywords: Anti-HLA antibodies, beads-based assay, cell-based assay, complement-dependent cytotoxicity crossmatch, flow cytometry crossmatch, luminex crossmatch, Luminex Single Antigen technology, monoclonal antibodies, organ transplantation, solid-phase assay, virtual crossmatch, XM-ONE

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Article Details

Year: 2012
Published on: 23 April, 2012
Page: [34 - 39]
Pages: 6
DOI: 10.2174/187152212799857646

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