Biopharmaceutical properties together with potency contribute critically towards clinical efficacy of the drugs by
influencing the dissolution and bioavailability. The aim of this study was to develop an amphiphilic phyto-phospholipid complex
in order to enhance the delivery of poorly soluble rutin. The rutin-phospholipid complex (Ru-PLc) was prepared and investigated
for various physico-chemical parameters like drug loading, infrared absorption (FTIR), differential scanning calorimetry
(DSC), X-ray powder diffractometry (XRPD), scanning electron microscopy (SEM), aqueous/ n-octanol solubility
and dissolution study. The in vitro anti-oxidant activity was also studied. In the SEM, Ru-PLc was found fluffy and porous
with rough surface morphology. FTIR, DSC and XRPD data confirmed the formation of phospholipid complex. The water/ noctanol
solubility of rutin was improved from 2.88 to 45.71 μg/ ml and 68.17 to 245.18 μg/ ml, respectively in the complex.
The improved dissolution was shown by the phospholipid complex at different pH buffers. The antioxidant activity indicated
that, the bioactivity of rutin was maintained even after being complexed with the phospholipid. Based on the results, it can be
concluded that the phospholipid complex may be considered as a promising drug delivery system for improving the overall
absorption and bioavailability of the rutin molecule.