State-of-the-Art Methodologies for the Discovery and Characterization of DNA G-Quadruplex Binders

Author(s): Bruno Pagano, Sandro Cosconati, Valerie Gabelica, Luigi Petraccone, Stefano De Tito, Luciana Marinelli, Valeria La Pietra, Francesco Saverio di Leva, Ilaria Lauri, Roberta Trotta, Ettore Novellino, Concetta Giancola, Antonio Randazzo

Journal Name: Current Pharmaceutical Design

Volume 18 , Issue 14 , 2012

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Abstract:

Nowadays, the molecular basis of interaction between low molecular weight compounds and biological macromolecules is the subject of numerous investigations aimed at the rational design of molecules with specific therapeutic applications. In the last decades, it has been demonstrated that DNA quadruplexes play a critical role in several biological processes both at telomeric and gene promoting levels thus providing a great stride in the discovery of ligands able to interact with such a biologically relevant DNA conformation. So far, a number of experimental and computational approaches have been successfully employed in order to identify new ligands and to characterize their binding to the DNA. The main focus of this review is the description of these methodologies, placing a particular emphasis on computational methods, isothermal titration calorimetry (ITC), mass spectrometry (MS), nuclear magnetic resonance (NMR), circular dichroism (CD) and fluorescence spectroscopies.

Keywords: G-quadruplex, isothermal titration calorimetry (ITC), fluorescence, nuclear magnetic resonance (NMR), circular dichroism (CD), computational methods, mass spectrometry (MS), immortalization, stabilization, quarfloxin

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Article Details

VOLUME: 18
ISSUE: 14
Year: 2012
Page: [1880 - 1899]
Pages: 20
DOI: 10.2174/138161212799958332
Price: $65

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