Group A Streptococcal Vaccine Candidates based on the Conserved Conformational Epitope from M Protein

Author(s): Mariusz Skwarczynski, Abdullah A. H. Ahmad Fuaad, Lina Rustanti, Zyta M. Ziora, Mohammed Aqil, Michael R Batzloff, Michael F. Good, Istvan Toth

Journal Name: Drug Delivery Letters

Volume 1 , Issue 1 , 2011

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Copper catalysed azide alkyne Huisgen cycloaddition (click) reaction assisted conjugation of the multiple copy of conserve epitope (J14) derived from Group A Streptococcal M-protein to LCP core induced desired α-helical conformation of the epitope. The constructs were able to self assemble into large nanoparticles under aqueous conditions. Immunological assessment of these particles demonstrated their capacity to elicit high-titer systemic IgG antibodies against the epitope without help of adjuvant. The LCP systems incorporating the epitope via its N- or C-termini did not elicit significantly different immune responses.

Keywords: Epitope orientation, group A streptococcus, infectious disease, lipid core peptide, nanoparticles, self-adjuvanting, lipopeptide vaccine, N-TERMINUS J14 AZIDE, HUISGEN CYCLOADDITION, LCP CORE

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Article Details

Year: 2011
Published on: 01 March, 2012
Page: [2 - 8]
Pages: 7
DOI: 10.2174/2210304x11101010002

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