Abstract
Various protein factors, including telomerase and WRN helicase, are involved in telomere maintenance. Resveratrol (Rsv), a polyphenol that extends the lifespan of diverse species is an activator of SIRT1, a NAD+ dependent deacetylating enzyme in mammalian cells. Here, we examined the changes in gene expressions and promoter activities of WRN helicase and telomerase after Rsv treatment. This treatment increased the amount of WRN transcript and protein product by activating its promoter and telomerase promoter activity and gene expression. However cell proliferation was not changed. This suggests that Rsv induces telomere maintenance factors like WRN helicase without affecting cell proliferation.
Keywords: BLM, Resveratrol, Telomerase, Sp1, TERT, WRN, Werner Syndrome, deacetylating enzyme, cell proliferation, eukaryotic chromosomes, fibroblasts, Reactive oxygen species (ROS), mitochondrial respiration, caloric restriction (CR), superoxide dismutases (SODs), Human cervical carcinoma (HeLa S3), Dulbecco's modified Eagle's (DME) medium, MTS assay, Luciferase, plasmids, DEAE-dextran method, Reverse Transcriptase Polymerase Chain Reaction (RTPCR), acrylamide gels, ethidium bromide, Real time PCR analysis, GAPDH, plasmid-transfected cells, anti-actin, anti-Sp1 antibodies, DNA-damage responses, DNA-repair synthesis, insulin signaling, epigenetic, sirtuin (SIRT1)-mediated deacetylation
Current Aging Science
Title: The Effect of Resveratrol on the Werner Syndrome RecQ Helicase Gene and Telomerase Activity
Volume: 4 Issue: 1
Author(s): Fumiaki Uchiumi, Takeshi Watanabe, Shin Hasegawa, Taisuke Hoshi, Yoshikazu Higami and Sei-ichi Tanuma
Affiliation:
Keywords: BLM, Resveratrol, Telomerase, Sp1, TERT, WRN, Werner Syndrome, deacetylating enzyme, cell proliferation, eukaryotic chromosomes, fibroblasts, Reactive oxygen species (ROS), mitochondrial respiration, caloric restriction (CR), superoxide dismutases (SODs), Human cervical carcinoma (HeLa S3), Dulbecco's modified Eagle's (DME) medium, MTS assay, Luciferase, plasmids, DEAE-dextran method, Reverse Transcriptase Polymerase Chain Reaction (RTPCR), acrylamide gels, ethidium bromide, Real time PCR analysis, GAPDH, plasmid-transfected cells, anti-actin, anti-Sp1 antibodies, DNA-damage responses, DNA-repair synthesis, insulin signaling, epigenetic, sirtuin (SIRT1)-mediated deacetylation
Abstract: Various protein factors, including telomerase and WRN helicase, are involved in telomere maintenance. Resveratrol (Rsv), a polyphenol that extends the lifespan of diverse species is an activator of SIRT1, a NAD+ dependent deacetylating enzyme in mammalian cells. Here, we examined the changes in gene expressions and promoter activities of WRN helicase and telomerase after Rsv treatment. This treatment increased the amount of WRN transcript and protein product by activating its promoter and telomerase promoter activity and gene expression. However cell proliferation was not changed. This suggests that Rsv induces telomere maintenance factors like WRN helicase without affecting cell proliferation.
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Cite this article as:
Uchiumi Fumiaki, Watanabe Takeshi, Hasegawa Shin, Hoshi Taisuke, Higami Yoshikazu and Tanuma Sei-ichi, The Effect of Resveratrol on the Werner Syndrome RecQ Helicase Gene and Telomerase Activity, Current Aging Science 2011; 4 (1) . https://dx.doi.org/10.2174/1874609811104010001
DOI https://dx.doi.org/10.2174/1874609811104010001 |
Print ISSN 1874-6098 |
Publisher Name Bentham Science Publisher |
Online ISSN 1874-6128 |
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