Dissecting Virus-Plant Interactions Through Proteomics Approaches

Kai      Xu; Peter      D. Nagy

Abstract

Plant viruses exploit cellular factors, including host proteins, membranes and metabolites, for their replication in infected cells and to establish systemic infections. Besides traditional genetic, molecular, cellular and biochemical methods for studying plant-virus interactions, both global and specialized proteomics methods are emerging as useful approaches for the identification of all the host proteins that play roles in virus infections. The various proteomics approaches include measuring differential protein expression in virus-infected versus noninfected cells, analysis of viral and host protein components in the viral replicase or other virus-induced complexes, as well as proteome-wide screens to identify host protein - viral protein interactions using protein arrays or yeast two-hybrid assays. In this review, we will discuss the progress made in plant virology using various proteomics methods, and highlight the functions of some of the identified host proteins during viral infections. Since global proteomics approaches do not usually identify the molecular mechanisms of the identified host factors during viral infections, additional experiments using genetics, biochemistry, cell biology and other approaches will often prove necessary to characterize the functions of host factors. Overall, the everimproving proteomics approaches promise further understanding of plant-virus interactions that will likely result in new strategies for viral disease control in plants.

Keywords: Proteome, protein microarray, plant, virus, host factors, protein-protein interaction, RNA binding, virus replication, host-virus interaction, Tobacco mosaic virus, Nicotiana benthamiana, Rice yellow mottle Virus, Tomato bushy stunt virus, Brome mosaic virus, Beta-1,3-Endoglucanase, pepper mild mottle virus, mRNAbinding protein, Rab11 GTPase, Ubiquitin, ribonucleoprotein, Cucumber necrosis virus, glyceraldehyde-3-phosphate dehydrogenase, pyruvate decarboxylase, potyvirus RNA dependent RNA polymerase, Turnip mosaic virus, Arabidopsis thaliana, heat shock cognate 70-3, AtHsc70-3, PABP, clamp adenine motif, eukaryotic translational elongation factor EF-1 alpha (eEF1A), Beet necrotic yellow vein virus, bimolecular fluorescence complementation assay, Potato virus X, Cyclosporin Asensitive proline rotamase 1, Cyclophilins, Tomato Mosaic virus, REACTOME