Abstract
Since its introduction in 2002 ‘stable isotope labeling by amino acids in cell culture’ (SILAC) has become a major tool for quantitative proteomics. Stable isotopes are incorporated into proteins by introduction of labeled amino acids in growth medium. This methodologys compatibility with virtually all cell culture conditions, ease of implementation into existing workflows and the high quality quantitative data that can be obtained have made it the quantitative method of choice for many laboratories. Although originally used for mammalian cell cultures, it has been adapted to a number of organisms including yeast, bacteria, plants and higher organisms. This review will look at the use of SILAC as a key tool in quantitative biology. The purpose of this review is to furnish the reader with a basic understanding of the fundamental principles of SILAC including its implementation, application, bioinformatic tools for its analyses and potential problems that one should be mindful of.
Keywords: Stable isotope labeling by amino acids in cell culture, SILAC, proteomics, mass spectrometry, Enzymatic, Peptides (iTRAQ), Proteolytic Peptides, Saccharomyces Cerevisiae, Deuterium, Thiol-Specific, Tri-Deuterium, Escherichia Coli, LC-MS/MS, MALDI-MS, Phosphorylation
Current Proteomics
Title: Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) – an Introduction for Biologists
Volume: 8 Issue: 1
Author(s): Robert L.J. Graham, Michael J. Sweredoski and Sonja Hess
Affiliation:
Keywords: Stable isotope labeling by amino acids in cell culture, SILAC, proteomics, mass spectrometry, Enzymatic, Peptides (iTRAQ), Proteolytic Peptides, Saccharomyces Cerevisiae, Deuterium, Thiol-Specific, Tri-Deuterium, Escherichia Coli, LC-MS/MS, MALDI-MS, Phosphorylation
Abstract: Since its introduction in 2002 ‘stable isotope labeling by amino acids in cell culture’ (SILAC) has become a major tool for quantitative proteomics. Stable isotopes are incorporated into proteins by introduction of labeled amino acids in growth medium. This methodologys compatibility with virtually all cell culture conditions, ease of implementation into existing workflows and the high quality quantitative data that can be obtained have made it the quantitative method of choice for many laboratories. Although originally used for mammalian cell cultures, it has been adapted to a number of organisms including yeast, bacteria, plants and higher organisms. This review will look at the use of SILAC as a key tool in quantitative biology. The purpose of this review is to furnish the reader with a basic understanding of the fundamental principles of SILAC including its implementation, application, bioinformatic tools for its analyses and potential problems that one should be mindful of.
Export Options
About this article
Cite this article as:
L.J. Graham Robert, J. Sweredoski Michael and Hess Sonja, Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) – an Introduction for Biologists, Current Proteomics 2011; 8 (1) . https://dx.doi.org/10.2174/157016411794697354
DOI https://dx.doi.org/10.2174/157016411794697354 |
Print ISSN 1570-1646 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6247 |
Call for Papers in Thematic Issues
Mass spectrometry data acquisition and analysis for proteomics
The Thematic Issue on "Mass spectrometry data acquisition and analysis for proteomics" aims to explore the latest advancements and challenges in the field of proteomics through the lens of mass spectrometry. Proteomics, the large-scale study of proteins and their functions, plays a crucial role in understanding various biological processes and ...read more
Peptides: State-of-Art and Commercialisation Hurdles
The Editors of the Current Proteomics (CP) journal are highly privileged to welcome scientists to submit their scientific research and review articles to be considered for publication in the upcoming thematic issue. The topics should cover various aspects of peptides in regard to their synthetic methodologies, formulation approaches, pharmacological challenges, ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Tyrosine Hydroxylase Gene: Another Piece of the Genetic Puzzle of Parkinson’s Disease
CNS & Neurological Disorders - Drug Targets Indian Spices for Healthy Heart - An Overview
Current Cardiology Reviews Gut Microbiota as an Emerging Therapeutic Avenue for the Treatment of Nonalcoholic Fatty Liver Disease
Current Pharmaceutical Design Sulforaphane Protects Small Intestinal Mucosa From Aspirin/NSAID-Induced Injury by Enhancing Host Defense Systems Against Oxidative Stress and by Inhibiting Mucosal Invasion of Anaerobic Enterobacteria
Current Pharmaceutical Design Modulation of Cell Death in Age-Related Diseases
Current Pharmaceutical Design Application of Genetic Polymorphisms in DNA Repair in the Prediction of Cancer Susceptibility and Clinical Outcome
Current Pharmacogenomics Hypericin and its Derivatives Act as Radiosensitizing Agents That Can Inhibit Tumor Initiating Cell Viability
Clinical Cancer Drugs Current Advances in the Synthesis and Antitumoral Activity of SIRT1-2 Inhibitors by Modulation of p53 and Pro-Apoptotic Proteins
Current Medicinal Chemistry Assays for Identification of Hsp90 Inhibitors and Biochemical Methods for Discriminating their Mechanism of Action
Current Topics in Medicinal Chemistry Bioactive Proteins and Peptides from Food Sources. Applications of Bioprocesses used in Isolation and Recovery
Current Pharmaceutical Design Quassinoids: From Traditional Drugs to New Cancer Therapeutics
Current Medicinal Chemistry Design and Synthesis of New Selective P-gp Substrates and Inhibitors
Current Pharmaceutical Design Anticancer Effect of Amygdalin (Vitamin B-17) on Hepatocellular Carcinoma Cell Line (HepG2) in the Presence and Absence of Zinc
Anti-Cancer Agents in Medicinal Chemistry Novel dibenzo[b,d]furan–1H-1,2,4-triazole derivatives: Synthesis and antitumor activity
Anti-Cancer Agents in Medicinal Chemistry Biomarkers in Amyotrophic Lateral Sclerosis: Is There A Neurovascular Pathway?
Current Neurovascular Research Choice of Biologic Therapy for Patients with Rheumatoid Arthritis: The Infection Perspective
Current Rheumatology Reviews ATP Citrate Lyase (ACLY): A Promising Target for Cancer Prevention and Treatment
Current Drug Targets A Review of Natural and Synthetic Antioxidants Important for Health and Longevity
Current Medicinal Chemistry Pomegranate Juice does not Affect the Bioavailability of Cyclosporine in Healthy Thai Volunteers
Current Clinical Pharmacology Hypoxia-Inducible Factor: A Potential Therapeutic Target for Rheumatoid Arthritis
Current Drug Targets