The Role of HIV-1 DNA as an Additional Marker of HIV-1 Infection

Author(s): Apostolos Beloukas, Dimitrios Paraskevis, Mina Psichogiou, Angelos Hatzakis

Journal Name: Current HIV Research
HIV and Viral Immune Diseases

Volume 7 , Issue 3 , 2009


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Abstract:

After the infusion of HIV-1 virus into a host cell, RNA is reverse transcribed to dsDNA, which persists intracellular to the infected cell in a variety of forms. Numerous in-house assays have been developed for the quantification of the different cellular HIV-1 DNA forms; these implement conventional or real-time PCR methodology. In this review we discuss recent findings about the longitudinal monitoring of cell-associated HIV-1 DNA in naïve and pre-treated patients, as a marker for clinical progression, treatment initiation and long-term success of HAART. These findings underline the importance of monitoring HIV-1 DNA in clinical practice, in addition to HIV-RNA and CD4+ T Cell counts, for the better assessment of HIV-treatment and disease progression. The lack of a standardized real-time PCR assay is major impediment to more wide-spread HIV-1 DNA monitoring.

Keywords: HIV-1 DNA, Real-time PCR, Prognostic marker, Disease progression

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Article Details

VOLUME: 7
ISSUE: 3
Year: 2009
Published on: 01 March, 2012
Page: [255 - 265]
Pages: 11
DOI: 10.2174/157016209788348001
Price: $65

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