Abstract
Protein phosphorylation is a key post-translational modification that controls intracellular signalling in virtually all cell types. In the nervous system, it contributes to the regulation of neuronal signalling and control processes underlying synaptic plasticity and cognitive functions. However, despite its importance, knowledge about phosphoproteins and their phosphosites in the brain remains limited. A pre-requisite for unravelling brain biology and function at the molecular level, are the qualitative and quantitative analyses of protein phosphorylation and its dynamics. These analyses of the phosphoproteome require novel methodologies in addition to traditional biochemical methods. Current phosphoproteomic workflows have reached a level of maturity, which allow for their use in combination with molecular approaches, and their application to the study of higher order brain function and cognitive processes. Neuroproteomics is emerging as an essential new sub-field of the neurosciences. This review focuses on the recent advances in the application of neuroproteomics to the phosphoproteome and discusses the challenges to come.
Keywords: Phosphoproteomics, phosphorylation, phosphopeptide enrichment, stable isotope labelling, quantitative mass spectrometry, neuroproteomics, nerve cell/neuron
Current Proteomics
Title: Neuroproteomics and the Detection of Regulatory Phosphosites
Volume: 4 Issue: 4
Author(s): Ry Y. Tweedie-Cullen, Bernd Wollscheid, Magdalena Livingstone-Zatchej and Isabelle M. Mansuy
Affiliation:
Keywords: Phosphoproteomics, phosphorylation, phosphopeptide enrichment, stable isotope labelling, quantitative mass spectrometry, neuroproteomics, nerve cell/neuron
Abstract: Protein phosphorylation is a key post-translational modification that controls intracellular signalling in virtually all cell types. In the nervous system, it contributes to the regulation of neuronal signalling and control processes underlying synaptic plasticity and cognitive functions. However, despite its importance, knowledge about phosphoproteins and their phosphosites in the brain remains limited. A pre-requisite for unravelling brain biology and function at the molecular level, are the qualitative and quantitative analyses of protein phosphorylation and its dynamics. These analyses of the phosphoproteome require novel methodologies in addition to traditional biochemical methods. Current phosphoproteomic workflows have reached a level of maturity, which allow for their use in combination with molecular approaches, and their application to the study of higher order brain function and cognitive processes. Neuroproteomics is emerging as an essential new sub-field of the neurosciences. This review focuses on the recent advances in the application of neuroproteomics to the phosphoproteome and discusses the challenges to come.
Export Options
About this article
Cite this article as:
Tweedie-Cullen Y. Ry, Wollscheid Bernd, Livingstone-Zatchej Magdalena and Mansuy M. Isabelle, Neuroproteomics and the Detection of Regulatory Phosphosites, Current Proteomics 2007; 4 (4) . https://dx.doi.org/10.2174/157016407783221240
DOI https://dx.doi.org/10.2174/157016407783221240 |
Print ISSN 1570-1646 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6247 |
Call for Papers in Thematic Issues
Mass spectrometry data acquisition and analysis for proteomics
The Thematic Issue on "Mass spectrometry data acquisition and analysis for proteomics" aims to explore the latest advancements and challenges in the field of proteomics through the lens of mass spectrometry. Proteomics, the large-scale study of proteins and their functions, plays a crucial role in understanding various biological processes and ...read more
Peptides: State-of-Art and Commercialisation Hurdles
The Editors of the Current Proteomics (CP) journal are highly privileged to welcome scientists to submit their scientific research and review articles to be considered for publication in the upcoming thematic issue. The topics should cover various aspects of peptides in regard to their synthetic methodologies, formulation approaches, pharmacological challenges, ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Localised Delivery of Therapeutic Agents to CNS Malignancies: Old and New Approaches
Current Pharmaceutical Biotechnology Nanotechnology in Therapeutics – Current Technologies and Applications
Current Nanoscience Can Environmentally Relevant Levels of Aluminium Promote the Onset and Progression of Neurodegenerative Diseases?
Current Inorganic Chemistry (Discontinued) NMNAT Expression and its Relation to NAD Metabolism
Current Medicinal Chemistry The Yin and Yang of Inflammation
Current Molecular Medicine Potential Use of Nanomedicine for Drug Delivery Across the Blood-Brain Barrier in Healthy and Diseased Brain
CNS & Neurological Disorders - Drug Targets Epigenetic Drugs for Multiple Sclerosis
Current Neuropharmacology Activation of the dsRNA-Activated Protein Kinase PKR in Mitochondrial Dysfunction and Inflammatory Stress in Metabolic Syndrome
Current Pharmaceutical Design Viral Induced Oxidative and Inflammatory Response in Alzheimer’s Disease Pathogenesis with Identification of Potential Drug Candidates: A Systematic Review using Systems Biology Approach
Current Neuropharmacology Sphingolipid Metabolism and Leukemia: A Potential for Novel Therapeutic Approaches
Anti-Cancer Agents in Medicinal Chemistry Experimental Autoimmune Encephalomyelitis (EAE) Model of Cynomolgus Macaques Induced by Recombinant Human MOG1-125 (rhMOG1-125) Protein and MOG34-56 Peptide
Protein & Peptide Letters Managing Neuropathic Pain in Multiple Sclerosis: Pharmacological Interventions
Medicinal Chemistry Induction of Oral Tolerance with Transgenic Plants Expressing Antigens for Prevention/Treatment of Autoimmune, Allergic and Inflammatory Diseases
Current Pharmaceutical Biotechnology A Systematic Review on the Role of Arachidonic Acid Pathway in Multiple Sclerosis
CNS & Neurological Disorders - Drug Targets Editorial: Current Strategies for Drug Discovery Targeting Neurological Autoimmune Diseases
Central Nervous System Agents in Medicinal Chemistry Effects of Biphenyl Sulfonylamino Methyl Bisphosphonic Acids on Porphyromonas Gingivalis and Cytokine Secretion by Oral Epithelial Cells
Medicinal Chemistry Microglia-Neuron Interaction in Inflammatory and Degenerative Diseases: Role of Cholinergic and Noradrenergic Systems
CNS & Neurological Disorders - Drug Targets T Cell Replicative Senescence in Human Aging
Current Pharmaceutical Design New Actors for the Immunological Mechanisms Involved in the Materno- Fetal Tolerance
Current Women`s Health Reviews Borna Disease Virus (BDV): Neuropharmacological Effects of a CNS Viral Infection
Current Neuropharmacology