Cell-free and Cell-based Methods for Characterizing Nuclear Receptor Ligands

Author(s): Richard K. Harrison, Siqi Lin, H. Martin Seidel, Ji-hu Zhang, Litao Zhang, Gerard M. McGeehan

Journal Name: Current Medicinal Chemistry - Immunology, Endocrine & Metabolic Agents
Continued as Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry

Volume 2 , Issue 1 , 2002


This article will review both the binding and the functional cellular assays that can be used to characterize nuclear receptor ligands in vitro. The discussion will be broken into two sections, concentrating on cell-free and cell-based systems. Each section will provide an overview of available assays moving from generic screening assays to more specific technologies that can be used to discriminate the pharmacology of receptor / ligand interactions.

Keywords: nuclear receptor ligands, radiometric assays, cell-based assays, gal4-lbd chimeras

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Article Details

Year: 2002
Page: [23 - 31]
Pages: 9
DOI: 10.2174/1568013024606459

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PDF: 15