Abstract
Proteomic approaches play an important role in the study of complex biological systems. The application of proteomic technologies in plant science has been strongly supported by the completion of genome sequence projects of the model plants Arabidopsis thaliana and rice. This review focuses on the state of proteomic technologies with special emphasis on their application in plant biology. An overview of recent developments in 2-dimensional gel electrophoresis and liquid chromatography-based multidimensional protein identification technology, MudPIT is provided. These techniques are commonly combined with mass spectrometric methods for identification of proteins. Furthermore, protein expression profiling by antibody arrays and the selection of required recombinant antibodies by phage display are described. Interaction studies, using functional protein microarrays or the yeast two-hybrid system are presented as powerful techniques to gain insights into the function of proteins. Advantages and limitations of the described methods as well as their current and potential future applications in plant research are discussed.
Keywords: proteomics, yeast two-hybrid, 2-d electrophoresis, plant, protein microarray, antibody microarray, phage display, mass spectrometry
Current Proteomics
Title: Proteomic Approaches in Plant Biology
Volume: 1 Issue: 2
Author(s): Birgit Kersten, Tanja Feilner, Philipp Angenendt, Patrick Giavalisco, Wolfram Brenner and Lukas Burkle
Affiliation:
Keywords: proteomics, yeast two-hybrid, 2-d electrophoresis, plant, protein microarray, antibody microarray, phage display, mass spectrometry
Abstract: Proteomic approaches play an important role in the study of complex biological systems. The application of proteomic technologies in plant science has been strongly supported by the completion of genome sequence projects of the model plants Arabidopsis thaliana and rice. This review focuses on the state of proteomic technologies with special emphasis on their application in plant biology. An overview of recent developments in 2-dimensional gel electrophoresis and liquid chromatography-based multidimensional protein identification technology, MudPIT is provided. These techniques are commonly combined with mass spectrometric methods for identification of proteins. Furthermore, protein expression profiling by antibody arrays and the selection of required recombinant antibodies by phage display are described. Interaction studies, using functional protein microarrays or the yeast two-hybrid system are presented as powerful techniques to gain insights into the function of proteins. Advantages and limitations of the described methods as well as their current and potential future applications in plant research are discussed.
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Cite this article as:
Kersten Birgit, Feilner Tanja, Angenendt Philipp, Giavalisco Patrick, Brenner Wolfram and Burkle Lukas, Proteomic Approaches in Plant Biology, Current Proteomics 2004; 1 (2) . https://dx.doi.org/10.2174/1570164043379389
DOI https://dx.doi.org/10.2174/1570164043379389 |
Print ISSN 1570-1646 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6247 |
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