Annotation of the Human Genome by High-Throughput Sequence Analysis of Naturally Occurring Proteins

Author(s): Simon J. McGowan, Jonathan Terrett, Clive G. Brown, Paul J. Adam, Louise Aldridge, Jason C. Allen, Bob Amess, Kristian A. Andrews, Martin Barnes, David E. Barnwell

Journal Name: Current Proteomics

Volume 1 , Issue 1 , 2004

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The identification of protein-coding genes is currently based on the merging of evidence and predictions from a variety of databases that may themselves contain inaccurate and partial information. We have developed a method for mapping accurate interpretations of protein MS-MS data to the genome. This approach enables verification of genes, exons, transcripts and variant transcripts as well as the de novo discovery of novel protein-coding genes. Here we describe improvements in spectral interpretation algorithms, multiple separation techniques, sub-cellular fractionation and novel bioinformatics approaches to characterise more than 14,000 naturally occurring human genes.

Keywords: proteomics, genome, human, genes, protein isoforms, bioinformatics

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Article Details

Year: 2004
Page: [41 - 48]
Pages: 8
DOI: 10.2174/1570164043488298

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