Title: Defining Viral Protein Interactomes Using the Yeast Two-Hybrid Assay
VOLUME: 2 ISSUE: 3
Author(s):E. Diefenbach, A. L. Cunningham and R. J. Diefenbach
Affiliation:Centre for Virus Research,Westmead Millennium Institute, PO Box 412, Westmead 2145 NSW, Australia.
Keywords:Yeast two-hybrid assay, interactome, virus, proteomics, drug targets
Abstract: The yeast two-hybrid assay has proved a powerful tool in identifying and characterising binary protein-protein interactions. Not only can it be used to map interacting protein domains, it can also be used to screen cDNA libraries with a desired bait to identify novel binding partners. A number of factors including ease of use, cost effectiveness and suitability for high throughput analysis have made yeast-two hybrid one of the assays of choice for defining protein-protein interaction networks or interactomes for a range of organisms. The focus of this review is on the definition of viral interactomes using the yeast two-hybrid assay and the relevance of such studies to our understanding of viral pathogenesis.
