Generic placeholder image

Medicinal Chemistry

Editor-in-Chief

ISSN (Print): 1573-4064
ISSN (Online): 1875-6638

Comparison of Deproteinization Methods Used Before TLC-DB and HPLC Analysis of Flumequine Residues in Milk

Author(s): Irena M. Choma, Edyta M. Grzelak and Barbara Majer – Dziedzic

Volume 8, Issue 1, 2012

Page: [95 - 101] Pages: 7

DOI: 10.2174/157340612799278423

Price: $65

Abstract

Seventeen various extraction procedures based on precipitation of proteins in milk samples spiked with flumequine were tested. Several criteria were taken into account, when choosing the most effective. The supernatants were analyzed by thin-layer chromatography – direct bioautography (TLC-DB) and high performance liquid chromatography (HPLC-UV). The results obtained from both methods indicate as the best the same deproteinization procedure. The addition of acetonitrile to milk in 1:1 volume proportions gave the highest concentration of flumequine in supernatant and prompt coagulation of proteins in milk samples.

Keywords: Antibiotic residues, Escherichia coli, Flumequine, Milk, HPLC, TLC, , DB, proteins, MRL, livestock, UV


Rights & Permissions Print Cite
© 2024 Bentham Science Publishers | Privacy Policy