Purification of a Chitin-Binding Protein from Moringa oleifera Seeds with Potential to Relieve Pain and Inflammation

Mirella      Leite Pereira; Hermogenes      David de Oliveira; Jose      Tadeu Abreu de Oliveira; Juliana      Menezes Gifoni; Raquel      de Oliveira Rocha; Daniele      de Oliveira Bezerra de Sousa; Ilka      Maria Vasconcelos

Abstract

Moringa oleifera Lam. is a perennial multipurpose tree that has been successfully used in folk medicine to cure several inflammatory processes. The aim of this study was to purify and characterize a chitin-binding protein from Moringa oleifera seeds, named Mo-CBP4, and evaluate its antinociceptive and anti-inflammatory effects in vivo. The protein was purified by affinity chromatography on chitin followed by ion exchange chromatography. Acetic acid-induced abdominal constrictions assay was used for the antinociceptive and anti-inflammatory activity assessments. Mo-CBP4 is a glycoprotein (2.9% neutral carbohydrate) composed of two protein subunits with apparent molecular masses of 28 and 18 kDa (9 kDa in the presence of reducing agent). The intraperitoneal injection of Mo-CBP4 (3.5 and 10 mg/kg) into mice 30 min before acetic acid administration potently and significantly reduced the occurrence of abdominal writhing in a dose dependent manner by 44.7% and 100%, respectively. In addition, the oral administration of the protein (10 mg/kg) resulted in 18% and 52.8% reductions in abdominal writhing when given 30 and 60 min prior to acetic acid administration, respectively. Mo-CBP4, when administered by intraperitoneal route, also caused a significant and dose-dependent inhibition of peritoneal capillary permeability induced by acid acetic and significantly inhibited leukocyte accumulation in the peritoneal cavity. In conclusion, this pioneering study describes that the chitin-binding protein Mo-CBP4, from M. oleifera seeds, exhibits anti-inflammatory and antinociceptive properties and scientifically supports the use of this multipurpose tree in folk medicine.

Keywords: Antinociceptive effect, anti-inflammatory activity, bioactivity, chitin-binding protein, Moringa oleifera, protein purification, Mo-CBP4, chromatography, Moringaceae family, aurantiamide acetate, ad libitum, CEPA, lyophilised, Polyacrylamide Gel Electrophoresis, Glycoprotein Detection KitAntinociceptive effect, anti-inflammatory activity, bioactivity, chitin-binding protein, Moringa oleifera, protein purification, Mo-CBP4, chromatography, Moringaceae family, aurantiamide acetate, ad libitum, CEPA, lyophilised, Polyacrylamide Gel Electrophoresis, Glycoprotein Detection Kit