Mass Spectrometry Characterization of Plant Phosphoproteins

Author(s): Katerina Novakova, Ondrej Sedo, Zbynek Zdrahal

Journal Name: Current Protein & Peptide Science

Volume 12 , Issue 2 , 2011

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Protein phosphorylation constitutes a major type of post-translational modifications mobilizing a high number of genes. It is involved in many crucial cell processes and largely participates in the features of the proteome. For several biological and technical reasons, the characterization of protein phosphorylation requires a combination of distinct procedures. In this review, a special emphasis is given to analytical strategies connected with the determination of the presence of phosphorylation sites and their localization by mass spectrometry (MS). The feasibility of selected combinations of analytical approaches for diverse objectives of phosphoproteomic research is discussed.

Keywords: Plant phosphorylation, phosphoproteomics, mass spectrometry, sample preparation, post-translational modifications, monitored protein separation, kinase inhibitors, spectrometric analysis., Liquid chromatography, lyophilization, Gel Electrophoresis, Chemical Tagging, Phospho-specific visualization, immunopurification

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Article Details

Year: 2011
Page: [112 - 125]
Pages: 14
DOI: 10.2174/138920311795684913
Price: $65

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