Title: Myoglobin as a New Fluorescence Probe to Sense H2S
VOLUME: 18 ISSUE: 3
Author(s):Maria Strianese, Franco De Martino, Claudio Pellecchia, Giuseppe Ruggiero and Sabato D'Auria
Affiliation:Laboratory for Molecular Sensing, IBP-CNR Via Pietro Castellino, 111 801131 Naples, Italy.
Keywords:Fluorescence, biosensors, FRET, heme-proteins, Sol-gelFluorescence, biosensors, FRET, heme-proteins, Sol-gel
Abstract: A new, fast, simple and cost-effective sensing device for monitoring H2S has been developed. Proof-of-principle results showing that a commercial and cheap Myoglobin (Mb) can be successfully used as a biological probe for a fluorescence biosensor for H2S detection are reported. Two different commercial labels Cy3 and Atto620 were selected for this study. A high selectivity for detecting H2S against other thiols was found. The applicability of the proposed sensing system was successfully explored not only in solution but also when applied in the form of a solid state device.
