Adsorption and Bioactivity of Tyrosine Hydroxylase on Gold Surfaces and Nanoparticles

Author(s): Oyvind Halskau, Sondre Volden, Ana C. Calvo, Aurora Martinez, Wilhelm R. Glomm

Journal Name: Protein & Peptide Letters

Volume 17 , Issue 11 , 2010

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Tyrosine hydroxylase is studied in terms of adsorption behaviour on gold surfaces and various passivating layers. Results reveal differences in layer formation, where mercaptoundecanoic acid-coated gold shows the best potential in terms of adsorbed mass. Nanoparticles with this coating are subsequently tested for enzymatic activity, which remains at attenuated levels.

Keywords: Gold nanoparticles, recombinant human tyrosine hydroxylase isoform 1 (hTH1), adsorption, stability, enzymatic activity, Parkinson's disease, nanoparticles, recombinant, enzymatic, Tyrosine Hydroxylase, (L-DOPA), (MUA), QCM-Z500, quartz crystals, Citrate, pKa, pH, electrostatic, kinetic, L-DOPA, TRANSIL, D-fplots, QCM

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Article Details

Year: 2010
Page: [1376 - 1382]
Pages: 7
DOI: 10.2174/0929866511009011376
Price: $65

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