Intein-Mediated Expression and Purification of an Analog of Glucagon-like Peptide-1 in Escherichia coli

Author(s): Chen Ma, Mingming Gao, Wenchao Liu, Jing Zhu, Hong Tian, Xiangdong Gao, Wenbing Yao

Journal Name: Protein & Peptide Letters

Volume 17 , Issue 10 , 2010

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Abstract:

To facilitate expression and purification of an analog of GLP-1 (mGLP-1), an intein system was employed in this study. A recombinant fusion protein, CBD-DnaB-mGLP-1, was constructed and expressed in the form of inclusion body. After refolding, the intein-mediated self-cleavage was triggered by pH and temperature shift. By using chitin beads column followed by single step purification, about 2.58 mg of mGLP-1 with the purity of up to 98% could be obtained from 1 L medium. Tricine-SDS-PAGE, RP-HPLC, and ESI-MS were undertaken to determine the purity and molecular weight of mGLP-1. The glucose-lowering activity of mGLP-1 was also preliminarily determined.

Keywords: Chitin beads, expression and purification, glucagon-like peptide-1, incretin, intein, self-cleavage

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Article Details

VOLUME: 17
ISSUE: 10
Year: 2010
Page: [1245 - 1250]
Pages: 6
DOI: 10.2174/092986610792231582
Price: $65

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