Functional Characterization of the In Vitro Folded Human Y1 Receptor in Lipid Environment

Author(s): Susann Schimmer, Diana Lindner, Peter Schmidt, Annette G. Beck-Sickinger, Daniel Huster, Rainer Rudolph

Journal Name: Protein & Peptide Letters

Volume 17 , Issue 5 , 2010

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Abstract:

We describe the recombinant production of the human Y1 receptor from inclusion bodies of E. coli cultures. The in vitro refolding was carried out in the presence of lipids from bovine brain extracts. Y1 receptors in brain lipids compete for cellular receptors in competitive binding experiments.

Keywords: GPCR, Y1 receptor, E. coli, in vitro folding, brain lipids

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Article Details

VOLUME: 17
ISSUE: 5
Year: 2010
Page: [605 - 609]
Pages: 5
DOI: 10.2174/092986610791112783
Price: $65

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